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Antiosteoporotic activity of echinacoside in ovariectomized rats.
Phytomedicine. 2013 Apr 15; 20(6):549-57.P

Abstract

PURPOSE

Echinacoside (ECH), isolated from Cistanche tubulosa (Schrenk) R. Wight stems, has been reported to enhance bone regeneration in MC3T3-E1 cells in vitro. The objectives of this study were to investigate the antiosteoporotic effect of ECH on bone metabolism in the ovariectomized (OVX) rat model of osteoporosis in vivo.

METHODS

Fifty-six aged 6 months female Sprague-Dawley rats were randomly assigned into sham-operated group (SHAM) and six OVX subgroups (n=8 each). The OVX rats were then subdivided into six groups treated with vehicle (OVX), Xian-ling-gu-bao (XLGB, 0.5 g/kg body weight/day, orally), 17β-estradiol (E2, 50 μg/kg body weight/day, orally) or ECH (30, 90, and 270 mg/kg body weight, daily, orally) for 12 weeks respectively. We evaluated the pharmacological effects of E2, XLGB and ECH against osteoporosis by evaluating the body weight, uterus wet weight, serum and urine biochemical parameters, bone mineral density (BMD), bone biomechanical properties, bone microarchitecture, bone histomorphology and uterus immunohistochemistry.

RESULTS

In OVX rats, the increases of body weight, serum hydroxyproline (HOP) levels, and the decreases of uterus wet weight and BMD were significantly reversed by ECH treatment. Moreover, three dosages of ECH completely corrected the increased urine concentration of calcium (Ca), inorganic phosphorus (P), and HOP observed in OVX rats. Furthermore, Micro-CT analysis results of distal femur showed that all ECH-treated groups notably enhanced bone quality compared to OVX group (p<0.05). Consistent with this finding, total femur BMD and biomechanical strength of tibia were significantly improved (p<0.05) after 12 weeks ECH administration. Histological results also showed the protective activity of ECH through promotion of bone formation and suppression of bone resorption. In addition, the ECH administration also significantly enhanced the expression of ER in the uteri according to immunohistochemical evaluation (p<0.05). Those findings, based on the serum and urine biochemical, BMD, Micro-CT, biomechanical test, histopathological and immunohistochemical parameters, showed that ECH has a notable antiosteoporotic effect, similar to estrogen, especially effective for prevention osteoporosis induced by estrogen deficiency.

CONCLUSION

These results suggest that ECH, as a new class of phytoestrogen, has a remarkable antiosteoporotic activity, and may be a promising candidate for treatment of postmenopausal osteoporosis induced by estrogen deficiency in a natural way through herbal resources.

Authors+Show Affiliations

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23428402

Citation

Li, Fei, et al. "Antiosteoporotic Activity of Echinacoside in Ovariectomized Rats." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 20, no. 6, 2013, pp. 549-57.
Li F, Yang X, Yang Y, et al. Antiosteoporotic activity of echinacoside in ovariectomized rats. Phytomedicine. 2013;20(6):549-57.
Li, F., Yang, X., Yang, Y., Guo, C., Zhang, C., Yang, Z., & Li, P. (2013). Antiosteoporotic activity of echinacoside in ovariectomized rats. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 20(6), 549-57. https://doi.org/10.1016/j.phymed.2013.01.001
Li F, et al. Antiosteoporotic Activity of Echinacoside in Ovariectomized Rats. Phytomedicine. 2013 Apr 15;20(6):549-57. PubMed PMID: 23428402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiosteoporotic activity of echinacoside in ovariectomized rats. AU - Li,Fei, AU - Yang,Xiaolin, AU - Yang,Yanan, AU - Guo,Changrun, AU - Zhang,Chunfeng, AU - Yang,Zhonglin, AU - Li,Ping, Y1 - 2013/02/18/ PY - 2012/10/10/received PY - 2012/12/09/revised PY - 2013/01/23/accepted PY - 2013/2/23/entrez PY - 2013/2/23/pubmed PY - 2013/9/24/medline SP - 549 EP - 57 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 20 IS - 6 N2 - PURPOSE: Echinacoside (ECH), isolated from Cistanche tubulosa (Schrenk) R. Wight stems, has been reported to enhance bone regeneration in MC3T3-E1 cells in vitro. The objectives of this study were to investigate the antiosteoporotic effect of ECH on bone metabolism in the ovariectomized (OVX) rat model of osteoporosis in vivo. METHODS: Fifty-six aged 6 months female Sprague-Dawley rats were randomly assigned into sham-operated group (SHAM) and six OVX subgroups (n=8 each). The OVX rats were then subdivided into six groups treated with vehicle (OVX), Xian-ling-gu-bao (XLGB, 0.5 g/kg body weight/day, orally), 17β-estradiol (E2, 50 μg/kg body weight/day, orally) or ECH (30, 90, and 270 mg/kg body weight, daily, orally) for 12 weeks respectively. We evaluated the pharmacological effects of E2, XLGB and ECH against osteoporosis by evaluating the body weight, uterus wet weight, serum and urine biochemical parameters, bone mineral density (BMD), bone biomechanical properties, bone microarchitecture, bone histomorphology and uterus immunohistochemistry. RESULTS: In OVX rats, the increases of body weight, serum hydroxyproline (HOP) levels, and the decreases of uterus wet weight and BMD were significantly reversed by ECH treatment. Moreover, three dosages of ECH completely corrected the increased urine concentration of calcium (Ca), inorganic phosphorus (P), and HOP observed in OVX rats. Furthermore, Micro-CT analysis results of distal femur showed that all ECH-treated groups notably enhanced bone quality compared to OVX group (p<0.05). Consistent with this finding, total femur BMD and biomechanical strength of tibia were significantly improved (p<0.05) after 12 weeks ECH administration. Histological results also showed the protective activity of ECH through promotion of bone formation and suppression of bone resorption. In addition, the ECH administration also significantly enhanced the expression of ER in the uteri according to immunohistochemical evaluation (p<0.05). Those findings, based on the serum and urine biochemical, BMD, Micro-CT, biomechanical test, histopathological and immunohistochemical parameters, showed that ECH has a notable antiosteoporotic effect, similar to estrogen, especially effective for prevention osteoporosis induced by estrogen deficiency. CONCLUSION: These results suggest that ECH, as a new class of phytoestrogen, has a remarkable antiosteoporotic activity, and may be a promising candidate for treatment of postmenopausal osteoporosis induced by estrogen deficiency in a natural way through herbal resources. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/23428402/Antiosteoporotic_activity_of_echinacoside_in_ovariectomized_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(13)00032-9 DB - PRIME DP - Unbound Medicine ER -