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Composition of hemagglutinin and neuraminidase affects the antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses.
Jpn J Infect Dis. 2013; 66(1):65-8.JJ

Abstract

To improve the hemagglutinin (HA) antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses, we generated 7:1, 6:2, and 5:3 genetic reassortant viruses between wild-type (H1N1)pdm09 (A/California/7/2009) (Cal7) and a high-yielding master virus, A/Puerto Rico/8/34 (PR8). These viruses contained the HA; HA and neuraminidase (NA); and HA, NA, and M genes, respectively, derived from Cal7, on a PR8 backbone. The influence of the amino acid residue at position 223 in Cal7 HA on virus growth and HA antigen yield differed between these reassortant viruses. NIIDRG-7, a 7:1 virus possessing arginine at position 223, exhibited a 10-fold higher 50% egg infectious dose (EID(50)) (10.0 log(10)EID(50)/ml) than the 5:3 and 6:2 viruses. It also had 1.5- to 3-fold higher protein (13.8 μg/ml of allantoic fluids) and HA antigen (4.1 μg/ml of allantoic fluids) yields than the 5:3 and 6:2 viruses, which possessed identical Cal7 HA proteins. However, the HA antigen yield of the other 7:1 virus, which possessed glutamine at position 223 was 60% of that of NIIDRG-7. In addition, a novel 6:2 virus possessing Cal7 HA and the NA of A/Wisconsin/10/98 (a triple reassortant swine-like H1N1 virus), produced 107% of the HA yield of NIIDRG-7. In this study, we showed that the balance between HA and NA in the influenza A(H1N1)pdm09 virus affects its protein and antigen yield.

Authors+Show Affiliations

Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 208-0011, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23429089

Citation

Shirakura, Masayuki, et al. "Composition of Hemagglutinin and Neuraminidase Affects the Antigen Yield of Influenza A(H1N1)pdm09 Candidate Vaccine Viruses." Japanese Journal of Infectious Diseases, vol. 66, no. 1, 2013, pp. 65-8.
Shirakura M, Kawaguchi A, Tashiro M, et al. Composition of hemagglutinin and neuraminidase affects the antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses. Jpn J Infect Dis. 2013;66(1):65-8.
Shirakura, M., Kawaguchi, A., Tashiro, M., & Nobusawa, E. (2013). Composition of hemagglutinin and neuraminidase affects the antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses. Japanese Journal of Infectious Diseases, 66(1), 65-8.
Shirakura M, et al. Composition of Hemagglutinin and Neuraminidase Affects the Antigen Yield of Influenza A(H1N1)pdm09 Candidate Vaccine Viruses. Jpn J Infect Dis. 2013;66(1):65-8. PubMed PMID: 23429089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Composition of hemagglutinin and neuraminidase affects the antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses. AU - Shirakura,Masayuki, AU - Kawaguchi,Akira, AU - Tashiro,Masato, AU - Nobusawa,Eri, PY - 2013/2/23/entrez PY - 2013/2/23/pubmed PY - 2015/1/6/medline SP - 65 EP - 8 JF - Japanese journal of infectious diseases JO - Jpn J Infect Dis VL - 66 IS - 1 N2 - To improve the hemagglutinin (HA) antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses, we generated 7:1, 6:2, and 5:3 genetic reassortant viruses between wild-type (H1N1)pdm09 (A/California/7/2009) (Cal7) and a high-yielding master virus, A/Puerto Rico/8/34 (PR8). These viruses contained the HA; HA and neuraminidase (NA); and HA, NA, and M genes, respectively, derived from Cal7, on a PR8 backbone. The influence of the amino acid residue at position 223 in Cal7 HA on virus growth and HA antigen yield differed between these reassortant viruses. NIIDRG-7, a 7:1 virus possessing arginine at position 223, exhibited a 10-fold higher 50% egg infectious dose (EID(50)) (10.0 log(10)EID(50)/ml) than the 5:3 and 6:2 viruses. It also had 1.5- to 3-fold higher protein (13.8 μg/ml of allantoic fluids) and HA antigen (4.1 μg/ml of allantoic fluids) yields than the 5:3 and 6:2 viruses, which possessed identical Cal7 HA proteins. However, the HA antigen yield of the other 7:1 virus, which possessed glutamine at position 223 was 60% of that of NIIDRG-7. In addition, a novel 6:2 virus possessing Cal7 HA and the NA of A/Wisconsin/10/98 (a triple reassortant swine-like H1N1 virus), produced 107% of the HA yield of NIIDRG-7. In this study, we showed that the balance between HA and NA in the influenza A(H1N1)pdm09 virus affects its protein and antigen yield. SN - 1884-2836 UR - https://www.unboundmedicine.com/medline/citation/23429089/Composition_of_hemagglutinin_and_neuraminidase_affects_the_antigen_yield_of_influenza_A_H1N1_pdm09_candidate_vaccine_viruses_ L2 - http://japanlinkcenter.org/DN/JST.JSTAGE/yoken/66.65?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -