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Capsaicin-induced activation of ERK1/2 and its involvement in GAP-43 expression and CGRP depletion in organotypically cultured DRG neurons.
Cell Mol Neurobiol. 2013 Apr; 33(3):433-41.CM

Abstract

Low concentrations of capsaicin (CAP) stimulate and high concentrations of CAP can be toxic to the primary sensory neurons of the dorsal root ganglion (DRG). CAP induces the phosphorylation of extracellular signal-regulated protein kinases 1/2 (ERK1/2) in DRG neurons. The effect of the activation of ERK1/2 by different concentrations of CAP on growth-associated protein 43 (GAP-43) expression and calcitonin gene-related peptide (CGRP) depletion in DRG neurons remains unknown. In the present study, organotypic embryonic 15-day-old rat DRG explants were used to determine the effect of different concentrations of CAP on GAP-43 expression and CGRP depletion. The results showed that, compared to unstimulated control cultures, GAP-43 and pERK1/2 protein levels increased at a low concentration (2 μmol/L) of CAP and decreased at a higher concentration (10 μmol/L). The number of CGRP-immunoreactive (IR) migrating neurons also decreased in CAP-treated cultures. The increase in GAP-43 levels and CGRP depletion could be blocked by the administration of ERK1/2 inhibitor PD98059. The results of the present study imply that CAP at different concentrations has different effects on GAP-43 expression and CGRP depletion. These effects were involved in the activation of ERK1/2 in organotypically cultured DRG neurons stimulated with CAP. These data may provide new insights for further development potential therapeutic applications of CAP with moderate dose on neurogenic inflammation.

Authors+Show Affiliations

Faculty of Clinical Medicine, Shandong University School of Medicine, Jinan, 250012, China. yunfengli630@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23430271

Citation

Li, Yunfeng, et al. "Capsaicin-induced Activation of ERK1/2 and Its Involvement in GAP-43 Expression and CGRP Depletion in Organotypically Cultured DRG Neurons." Cellular and Molecular Neurobiology, vol. 33, no. 3, 2013, pp. 433-41.
Li Y, Liu G, Li H, et al. Capsaicin-induced activation of ERK1/2 and its involvement in GAP-43 expression and CGRP depletion in organotypically cultured DRG neurons. Cell Mol Neurobiol. 2013;33(3):433-41.
Li, Y., Liu, G., Li, H., Xu, Y., Zhang, H., & Liu, Z. (2013). Capsaicin-induced activation of ERK1/2 and its involvement in GAP-43 expression and CGRP depletion in organotypically cultured DRG neurons. Cellular and Molecular Neurobiology, 33(3), 433-41. https://doi.org/10.1007/s10571-013-9909-8
Li Y, et al. Capsaicin-induced Activation of ERK1/2 and Its Involvement in GAP-43 Expression and CGRP Depletion in Organotypically Cultured DRG Neurons. Cell Mol Neurobiol. 2013;33(3):433-41. PubMed PMID: 23430271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capsaicin-induced activation of ERK1/2 and its involvement in GAP-43 expression and CGRP depletion in organotypically cultured DRG neurons. AU - Li,Yunfeng, AU - Liu,Guixiang, AU - Li,Hao, AU - Xu,Youzheng, AU - Zhang,Hong, AU - Liu,Zhen, Y1 - 2013/02/21/ PY - 2012/10/29/received PY - 2013/01/09/accepted PY - 2013/2/23/entrez PY - 2013/2/23/pubmed PY - 2013/9/5/medline SP - 433 EP - 41 JF - Cellular and molecular neurobiology JO - Cell Mol Neurobiol VL - 33 IS - 3 N2 - Low concentrations of capsaicin (CAP) stimulate and high concentrations of CAP can be toxic to the primary sensory neurons of the dorsal root ganglion (DRG). CAP induces the phosphorylation of extracellular signal-regulated protein kinases 1/2 (ERK1/2) in DRG neurons. The effect of the activation of ERK1/2 by different concentrations of CAP on growth-associated protein 43 (GAP-43) expression and calcitonin gene-related peptide (CGRP) depletion in DRG neurons remains unknown. In the present study, organotypic embryonic 15-day-old rat DRG explants were used to determine the effect of different concentrations of CAP on GAP-43 expression and CGRP depletion. The results showed that, compared to unstimulated control cultures, GAP-43 and pERK1/2 protein levels increased at a low concentration (2 μmol/L) of CAP and decreased at a higher concentration (10 μmol/L). The number of CGRP-immunoreactive (IR) migrating neurons also decreased in CAP-treated cultures. The increase in GAP-43 levels and CGRP depletion could be blocked by the administration of ERK1/2 inhibitor PD98059. The results of the present study imply that CAP at different concentrations has different effects on GAP-43 expression and CGRP depletion. These effects were involved in the activation of ERK1/2 in organotypically cultured DRG neurons stimulated with CAP. These data may provide new insights for further development potential therapeutic applications of CAP with moderate dose on neurogenic inflammation. SN - 1573-6830 UR - https://www.unboundmedicine.com/medline/citation/23430271/Capsaicin_induced_activation_of_ERK1/2_and_its_involvement_in_GAP_43_expression_and_CGRP_depletion_in_organotypically_cultured_DRG_neurons_ L2 - https://doi.org/10.1007/s10571-013-9909-8 DB - PRIME DP - Unbound Medicine ER -