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Endobronchial perfluorocarbon administration decreases lung injury in an experimental model of ischemia and reperfusion.
J Surg Res. 2013 Aug; 183(2):835-40.JS

Abstract

OBJECTIVE

To verify the effects of liquid endobronchial perfluorocarbon (PFC) administered before reperfusion in an animal model of lung ischemia-reperfusion injury.

METHODS

Eighteen Wistar rats were subjected to an experimental model of selective left pulmonary artery clamping for 45 min followed by reperfusion for 2 h. The animals were divided into three groups: the ischemia-reperfusion (IR) group, the sham group, and the PFC group. We recorded the hemodynamic parameters, blood gas analysis, and histology. A Western blot assay was used to measure the inducible nitric oxide synthase, caspase 3, and nuclear factor қB (subunit p65) activities. Lipid peroxidation was assessed by the thiobarbituric acid reactive substances assay and the activity of the antioxidant enzyme superoxide dismutase.

RESULTS

No significant differences were observed in lipid peroxidation among the groups. The superoxide dismutase activity was increased (P < 0.05) in the PFC-treated group. The expressions of nuclear factor қB, inducible nitric oxide synthase, and caspase 3 were significantly lower in the PFC group than in the IR group (P < 0.05). The histologic analysis showed a reduction in lung injuries in the PFC group compared with the sham and IR groups.

CONCLUSION

The use of endobronchial PFC reduces the inflammatory response, preserves the alveolar structure, and protects the lungs against the hazardous effects of ischemia-reperfusion injuries.

Authors+Show Affiliations

Postgraduate Program in Pulmonary Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23434305

Citation

Forgiarini, Luiz Alberto, et al. "Endobronchial Perfluorocarbon Administration Decreases Lung Injury in an Experimental Model of Ischemia and Reperfusion." The Journal of Surgical Research, vol. 183, no. 2, 2013, pp. 835-40.
Forgiarini LA, Forgiarini LF, da Rosa DP, et al. Endobronchial perfluorocarbon administration decreases lung injury in an experimental model of ischemia and reperfusion. J Surg Res. 2013;183(2):835-40.
Forgiarini, L. A., Forgiarini, L. F., da Rosa, D. P., Mariano, R., Ulbrich, J. M., & Andrade, C. F. (2013). Endobronchial perfluorocarbon administration decreases lung injury in an experimental model of ischemia and reperfusion. The Journal of Surgical Research, 183(2), 835-40. https://doi.org/10.1016/j.jss.2013.01.035
Forgiarini LA, et al. Endobronchial Perfluorocarbon Administration Decreases Lung Injury in an Experimental Model of Ischemia and Reperfusion. J Surg Res. 2013;183(2):835-40. PubMed PMID: 23434305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endobronchial perfluorocarbon administration decreases lung injury in an experimental model of ischemia and reperfusion. AU - Forgiarini,Luiz Alberto,Jr AU - Forgiarini,Luiz Felipe, AU - da Rosa,Darlan Pase, AU - Mariano,Rodrigo, AU - Ulbrich,Jane Maria, AU - Andrade,Cristiano Feijó, Y1 - 2013/02/09/ PY - 2012/10/16/received PY - 2013/01/12/revised PY - 2013/01/17/accepted PY - 2013/2/26/entrez PY - 2013/2/26/pubmed PY - 2013/10/18/medline KW - Apoptosis KW - Ischemia KW - Lung injury KW - Perfluorocarbon KW - Reperfusion SP - 835 EP - 40 JF - The Journal of surgical research JO - J Surg Res VL - 183 IS - 2 N2 - OBJECTIVE: To verify the effects of liquid endobronchial perfluorocarbon (PFC) administered before reperfusion in an animal model of lung ischemia-reperfusion injury. METHODS: Eighteen Wistar rats were subjected to an experimental model of selective left pulmonary artery clamping for 45 min followed by reperfusion for 2 h. The animals were divided into three groups: the ischemia-reperfusion (IR) group, the sham group, and the PFC group. We recorded the hemodynamic parameters, blood gas analysis, and histology. A Western blot assay was used to measure the inducible nitric oxide synthase, caspase 3, and nuclear factor қB (subunit p65) activities. Lipid peroxidation was assessed by the thiobarbituric acid reactive substances assay and the activity of the antioxidant enzyme superoxide dismutase. RESULTS: No significant differences were observed in lipid peroxidation among the groups. The superoxide dismutase activity was increased (P < 0.05) in the PFC-treated group. The expressions of nuclear factor қB, inducible nitric oxide synthase, and caspase 3 were significantly lower in the PFC group than in the IR group (P < 0.05). The histologic analysis showed a reduction in lung injuries in the PFC group compared with the sham and IR groups. CONCLUSION: The use of endobronchial PFC reduces the inflammatory response, preserves the alveolar structure, and protects the lungs against the hazardous effects of ischemia-reperfusion injuries. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/23434305/Endobronchial_perfluorocarbon_administration_decreases_lung_injury_in_an_experimental_model_of_ischemia_and_reperfusion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(13)00061-9 DB - PRIME DP - Unbound Medicine ER -