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The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri.
Dev Comp Immunol. 2013 Jun; 40(2):142-7.DC

Abstract

The enhanced immunity against a second encounter with the particular pathogen has suggested the presence of "immune priming" in scallop. In the present study, the survival rate and expression patterns of five C-lectin isoforms from scallop Chlamys farreri were explored after "vaccination" of heat-killed Vibrio anguillarum or successively challenge with V. anguillarum and Micrococcus luteus. When scallops were challenged with live bacteria, the survival rate increased significantly only in the group firstly "vaccinated" with inactivated V. anguillarum and then challenged with live V. anguillarum compared with naive scallops (from 41% to 63.6%, P<0.05), showing enhanced protective effects of inactivated bacteria with "specificity". When scallops received the challenge with V. anguillarum, the mRNA expression level of five C-lectins in scallops which were immuned previously with heat-killed V. anguillarum peaked significantly higher (26.7-, 121.7-, 60.1-, 27.4-, 16.3-fold to 0h, respectively, P<0.01) than that in non-immuned scallops (7.6-fold, P<0.05; 6.4-, 3.9-fold, P>0.05; 5.7-fold, P<0.05; 11.7-fold, P<0.01, to 0h, respectively). A significantly higher peak and 3-9h earlier response of all C-lectins mRNA expression were observed after challenge with live V. anguillarum (26.7-, 121.7-, 60.1-, 26.4- and 16.3-fold to 0h, respectively, P<0.01), compared with those only received first injection with heat-killed V. anguillarum (1.6-fold, P>0.05; 8.3-fold, P<0.05; 5.2-fold, P>0.05; 14.5-fold, P<0.01; 4.3-fold, P>0.05, to 0h, respectively). The response of mRNA expression to the secondary encounter with the same bacteria was stronger than that of successively encounter with the different class of bacteria. It was obvious that the mRNA expression of C-lectins in scallops was significantly enhanced by the successive challenge of same species of bacteria with a certain degree of specificity. All the results suggested that C-lectins might be involved in some form of immune priming, and it might provide new insights into mechanism of invertebrate immune priming.

Authors+Show Affiliations

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23434464

Citation

Wang, Jingjing, et al. "The Response of mRNA Expression Upon Secondary Challenge With Vibrio Anguillarum Suggests the Involvement of C-lectins in the Immune Priming of Scallop Chlamys Farreri." Developmental and Comparative Immunology, vol. 40, no. 2, 2013, pp. 142-7.
Wang J, Wang L, Yang C, et al. The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri. Dev Comp Immunol. 2013;40(2):142-7.
Wang, J., Wang, L., Yang, C., Jiang, Q., Zhang, H., Yue, F., Huang, M., Sun, Z., & Song, L. (2013). The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri. Developmental and Comparative Immunology, 40(2), 142-7. https://doi.org/10.1016/j.dci.2013.02.003
Wang J, et al. The Response of mRNA Expression Upon Secondary Challenge With Vibrio Anguillarum Suggests the Involvement of C-lectins in the Immune Priming of Scallop Chlamys Farreri. Dev Comp Immunol. 2013;40(2):142-7. PubMed PMID: 23434464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri. AU - Wang,Jingjing, AU - Wang,Lingling, AU - Yang,Chuanyan, AU - Jiang,Qiufen, AU - Zhang,Huan, AU - Yue,Feng, AU - Huang,Mengmeng, AU - Sun,Zhibin, AU - Song,Linsheng, Y1 - 2013/02/19/ PY - 2012/11/12/received PY - 2013/02/01/revised PY - 2013/02/07/accepted PY - 2013/2/26/entrez PY - 2013/2/26/pubmed PY - 2013/10/30/medline SP - 142 EP - 7 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 40 IS - 2 N2 - The enhanced immunity against a second encounter with the particular pathogen has suggested the presence of "immune priming" in scallop. In the present study, the survival rate and expression patterns of five C-lectin isoforms from scallop Chlamys farreri were explored after "vaccination" of heat-killed Vibrio anguillarum or successively challenge with V. anguillarum and Micrococcus luteus. When scallops were challenged with live bacteria, the survival rate increased significantly only in the group firstly "vaccinated" with inactivated V. anguillarum and then challenged with live V. anguillarum compared with naive scallops (from 41% to 63.6%, P<0.05), showing enhanced protective effects of inactivated bacteria with "specificity". When scallops received the challenge with V. anguillarum, the mRNA expression level of five C-lectins in scallops which were immuned previously with heat-killed V. anguillarum peaked significantly higher (26.7-, 121.7-, 60.1-, 27.4-, 16.3-fold to 0h, respectively, P<0.01) than that in non-immuned scallops (7.6-fold, P<0.05; 6.4-, 3.9-fold, P>0.05; 5.7-fold, P<0.05; 11.7-fold, P<0.01, to 0h, respectively). A significantly higher peak and 3-9h earlier response of all C-lectins mRNA expression were observed after challenge with live V. anguillarum (26.7-, 121.7-, 60.1-, 26.4- and 16.3-fold to 0h, respectively, P<0.01), compared with those only received first injection with heat-killed V. anguillarum (1.6-fold, P>0.05; 8.3-fold, P<0.05; 5.2-fold, P>0.05; 14.5-fold, P<0.01; 4.3-fold, P>0.05, to 0h, respectively). The response of mRNA expression to the secondary encounter with the same bacteria was stronger than that of successively encounter with the different class of bacteria. It was obvious that the mRNA expression of C-lectins in scallops was significantly enhanced by the successive challenge of same species of bacteria with a certain degree of specificity. All the results suggested that C-lectins might be involved in some form of immune priming, and it might provide new insights into mechanism of invertebrate immune priming. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/23434464/The_response_of_mRNA_expression_upon_secondary_challenge_with_Vibrio_anguillarum_suggests_the_involvement_of_C_lectins_in_the_immune_priming_of_scallop_Chlamys_farreri_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(13)00033-5 DB - PRIME DP - Unbound Medicine ER -