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On-line preconcentration and determination of six sulfonylurea herbicides in cereals by MEKC with large-volume sample stacking and polarity switching.
Electrophoresis. 2013 May; 34(9-10):1304-11.E

Abstract

A new MEKC method with large-volume sample stacking and polarity switching was developed for on-line preconcentration and detection of sulfonylurea herbicide (SUH) residues in cereals, including nicosulfuron (NS), thifensulfuon (methyl) (TFM), tribenuron-methly (TBM), sulfometuron-methyl (SMM), pyrazosulfuron-ethyl (PSE), and chlorimuron-ethyl (CME). In order to achieve a high resolution and enrichment factor, several parameters were optimized, such as the pH of the running buffer, the concentration of the BGE and the SDS, the separate voltage, the sample size, the pH, and the electrolyte concentration of the sample. The optimal running buffer was composed of 30 mM borate and 80 mM SDS at pH 7.0. The borate concentration in the sample was 30 mM and the pH value of the sample was the same as that of the running buffer. The concentrating voltage and the separating voltage were -15 kV and 15 kV, respectively. The sample size was 1.455 kPa × 780 s (33.11 cm). Under the optimum conditions, for NS, TFM, TBM, SMM, PSE, and CME, the enrichment factors were 613, 642, 835, 570, 709, and 599; the LODs were 0.29-0.50 ng/g, 0.22-0.36 ng/g, 0.60-0.89 ng/g, 0.39-0.72 ng/g, 0.28-0.56 ng/g, and 0.31-0.57 ng/g; the LOQs of six SUHs were all 5 ng/g; the average recoveries of the spiked sample were 86.68-92.99%, 80.73-93.65%, 81.49-94.40%, 82.97-95.1%, 82.96-98.84%, and 80.41-92.94%, respectively.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yi LX
College of Food and Bioengineering, Jiangsu University, Zhenjiang, China.
Chen GH
No affiliation info available
Fang R
No affiliation info available
Zhang L
No affiliation info available
Shao YX
No affiliation info available
Chen P
No affiliation info available
Tao XX
No affiliation info available

MeSH

BuffersChromatography, Micellar Electrokinetic CapillaryEdible GrainHerbicidesHydrogen-Ion ConcentrationLimit of DetectionSulfonylurea Compounds

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23436573

Citation

Yi, Ling-Xiao, et al. "On-line Preconcentration and Determination of Six Sulfonylurea Herbicides in Cereals By MEKC With Large-volume Sample Stacking and Polarity Switching." Electrophoresis, vol. 34, no. 9-10, 2013, pp. 1304-11.
Yi LX, Chen GH, Fang R, et al. On-line preconcentration and determination of six sulfonylurea herbicides in cereals by MEKC with large-volume sample stacking and polarity switching. Electrophoresis. 2013;34(9-10):1304-11.
Yi, L. X., Chen, G. H., Fang, R., Zhang, L., Shao, Y. X., Chen, P., & Tao, X. X. (2013). On-line preconcentration and determination of six sulfonylurea herbicides in cereals by MEKC with large-volume sample stacking and polarity switching. Electrophoresis, 34(9-10), 1304-11. https://doi.org/10.1002/elps.201200607
Yi LX, et al. On-line Preconcentration and Determination of Six Sulfonylurea Herbicides in Cereals By MEKC With Large-volume Sample Stacking and Polarity Switching. Electrophoresis. 2013;34(9-10):1304-11. PubMed PMID: 23436573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line preconcentration and determination of six sulfonylurea herbicides in cereals by MEKC with large-volume sample stacking and polarity switching. AU - Yi,Ling-Xiao, AU - Chen,Guan-Hua, AU - Fang,Rou, AU - Zhang,Li, AU - Shao,Yu-Xiu, AU - Chen,Ping, AU - Tao,Xiang-Xiang, Y1 - 2013/04/12/ PY - 2012/11/11/received PY - 2013/01/11/revised PY - 2013/01/11/accepted PY - 2013/2/26/entrez PY - 2013/2/26/pubmed PY - 2013/10/23/medline SP - 1304 EP - 11 JF - Electrophoresis JO - Electrophoresis VL - 34 IS - 9-10 N2 - A new MEKC method with large-volume sample stacking and polarity switching was developed for on-line preconcentration and detection of sulfonylurea herbicide (SUH) residues in cereals, including nicosulfuron (NS), thifensulfuon (methyl) (TFM), tribenuron-methly (TBM), sulfometuron-methyl (SMM), pyrazosulfuron-ethyl (PSE), and chlorimuron-ethyl (CME). In order to achieve a high resolution and enrichment factor, several parameters were optimized, such as the pH of the running buffer, the concentration of the BGE and the SDS, the separate voltage, the sample size, the pH, and the electrolyte concentration of the sample. The optimal running buffer was composed of 30 mM borate and 80 mM SDS at pH 7.0. The borate concentration in the sample was 30 mM and the pH value of the sample was the same as that of the running buffer. The concentrating voltage and the separating voltage were -15 kV and 15 kV, respectively. The sample size was 1.455 kPa × 780 s (33.11 cm). Under the optimum conditions, for NS, TFM, TBM, SMM, PSE, and CME, the enrichment factors were 613, 642, 835, 570, 709, and 599; the LODs were 0.29-0.50 ng/g, 0.22-0.36 ng/g, 0.60-0.89 ng/g, 0.39-0.72 ng/g, 0.28-0.56 ng/g, and 0.31-0.57 ng/g; the LOQs of six SUHs were all 5 ng/g; the average recoveries of the spiked sample were 86.68-92.99%, 80.73-93.65%, 81.49-94.40%, 82.97-95.1%, 82.96-98.84%, and 80.41-92.94%, respectively. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/23436573/On_line_preconcentration_and_determination_of_six_sulfonylurea_herbicides_in_cereals_by_MEKC_with_large_volume_sample_stacking_and_polarity_switching_ DB - PRIME DP - Unbound Medicine ER -
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