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Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae).
Toxicon. 2013 May; 66:64-74.T

Abstract

A new member of short chain α-neurotoxic protein family from venom of the Mexican coral snake, Micrurus laticollaris, was characterized. This protein, named MlatA1, possesses 61 amino acids with 8 conserved cysteine residues, sharing 30-91% sequence identity with other fully sequenced Micrurus toxins. MlatA1 (LD50i.v. = 0.064 mg/kg) antagonizes with both fetal and adult nicotinic acetylcholine receptor (nAChR) as well as α-7 neuronal nAChR in a dose-dependent way. Specific rabbit anti-Mlat serum (titer higher than 18,000) does not show any protective ability against this toxin, nevertheless it was able to recognize protein bands in six out of twelve Micrurus venoms showing the existence of two distinct antigenic groups for α-neurotoxins in North American coral snakes species. The MlatA1 gene was cloned and used to produce recombinant toxin (rMlatA1) that was recognized by rabbit anti-native toxin but was depleted of toxic activity.

Authors+Show Affiliations

Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP. 62210 Cuernavaca, Morelos, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23438486

Citation

Carbajal-Saucedo, Alejandro, et al. "Isolation, Characterization, Cloning and Expression of an Alpha-neurotoxin From the Venom of the Mexican Coral Snake Micrurus Laticollaris (Squamata: Elapidae)." Toxicon : Official Journal of the International Society On Toxinology, vol. 66, 2013, pp. 64-74.
Carbajal-Saucedo A, López-Vera E, Bénard-Valle M, et al. Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae). Toxicon. 2013;66:64-74.
Carbajal-Saucedo, A., López-Vera, E., Bénard-Valle, M., Smith, E. N., Zamudio, F., de Roodt, A. R., & Olvera-Rodríguez, A. (2013). Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae). Toxicon : Official Journal of the International Society On Toxinology, 66, 64-74. https://doi.org/10.1016/j.toxicon.2013.02.006
Carbajal-Saucedo A, et al. Isolation, Characterization, Cloning and Expression of an Alpha-neurotoxin From the Venom of the Mexican Coral Snake Micrurus Laticollaris (Squamata: Elapidae). Toxicon. 2013;66:64-74. PubMed PMID: 23438486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae). AU - Carbajal-Saucedo,Alejandro, AU - López-Vera,Estuardo, AU - Bénard-Valle,Melisa, AU - Smith,Eric N, AU - Zamudio,Fernando, AU - de Roodt,Adolfo R, AU - Olvera-Rodríguez,Alejandro, Y1 - 2013/02/22/ PY - 2012/09/27/received PY - 2013/01/25/revised PY - 2013/02/12/accepted PY - 2013/2/27/entrez PY - 2013/2/27/pubmed PY - 2013/9/4/medline SP - 64 EP - 74 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 66 N2 - A new member of short chain α-neurotoxic protein family from venom of the Mexican coral snake, Micrurus laticollaris, was characterized. This protein, named MlatA1, possesses 61 amino acids with 8 conserved cysteine residues, sharing 30-91% sequence identity with other fully sequenced Micrurus toxins. MlatA1 (LD50i.v. = 0.064 mg/kg) antagonizes with both fetal and adult nicotinic acetylcholine receptor (nAChR) as well as α-7 neuronal nAChR in a dose-dependent way. Specific rabbit anti-Mlat serum (titer higher than 18,000) does not show any protective ability against this toxin, nevertheless it was able to recognize protein bands in six out of twelve Micrurus venoms showing the existence of two distinct antigenic groups for α-neurotoxins in North American coral snakes species. The MlatA1 gene was cloned and used to produce recombinant toxin (rMlatA1) that was recognized by rabbit anti-native toxin but was depleted of toxic activity. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/23438486/Isolation_characterization_cloning_and_expression_of_an_alpha_neurotoxin_from_the_venom_of_the_Mexican_coral_snake_Micrurus_laticollaris__Squamata:_Elapidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(13)00067-6 DB - PRIME DP - Unbound Medicine ER -