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Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients.
Mem Inst Oswaldo Cruz. 2013 Feb; 108(1):116-8.MI

Abstract

Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus cantonensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-antibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand. praphathip.eam@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23440126

Citation

Eamsobhana, Praphathip, et al. "Molecular Diagnosis of Eosinophilic Meningitis Due to Angiostrongylus Cantonensis (Nematoda: Metastrongyloidea) By Polymerase Chain reaction-DNA Sequencing of Cerebrospinal Fluids of Patients." Memorias Do Instituto Oswaldo Cruz, vol. 108, no. 1, 2013, pp. 116-8.
Eamsobhana P, Wanachiwanawin D, Dechkum N, et al. Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients. Mem Inst Oswaldo Cruz. 2013;108(1):116-8.
Eamsobhana, P., Wanachiwanawin, D., Dechkum, N., Parsartvit, A., & Yong, H. S. (2013). Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients. Memorias Do Instituto Oswaldo Cruz, 108(1), 116-8.
Eamsobhana P, et al. Molecular Diagnosis of Eosinophilic Meningitis Due to Angiostrongylus Cantonensis (Nematoda: Metastrongyloidea) By Polymerase Chain reaction-DNA Sequencing of Cerebrospinal Fluids of Patients. Mem Inst Oswaldo Cruz. 2013;108(1):116-8. PubMed PMID: 23440126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients. AU - Eamsobhana,Praphathip, AU - Wanachiwanawin,Darawan, AU - Dechkum,Naowarat, AU - Parsartvit,Anchana, AU - Yong,Hoi Sen, PY - 2012/03/12/received PY - 2012/09/14/accepted PY - 2013/2/27/entrez PY - 2013/2/27/pubmed PY - 2013/11/1/medline SP - 116 EP - 8 JF - Memorias do Instituto Oswaldo Cruz JO - Mem Inst Oswaldo Cruz VL - 108 IS - 1 N2 - Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus cantonensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-antibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis. SN - 1678-8060 UR - https://www.unboundmedicine.com/medline/citation/23440126/Molecular_diagnosis_of_eosinophilic_meningitis_due_to_Angiostrongylus_cantonensis__Nematoda:_Metastrongyloidea__by_polymerase_chain_reaction_DNA_sequencing_of_cerebrospinal_fluids_of_patients_ L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100020&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -