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Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy.
Neurotherapeutics. 2013 Apr; 10(2):354-68.N

Abstract

Ventral spinal root avulsion causes complete denervation of muscles in the limb and also progressive death of segmental motoneurons (MN) leading to permanent paralysis. The chances for functional recovery after ventral root avulsion are very poor owing to the loss of avulsed neurons and the long distance that surviving neurons have to re-grow axons from the spinal cord to the corresponding targets. Following unilateral avulsion of L4, L5 and L6 spinal roots in adult rats, we performed an intraspinal transplant of mesenchymal stem cells (MSC) and surgical re-implantation of the avulsed roots. Four weeks after avulsion the survival of MN in the MSC-treated animals was significantly higher than in vehicle-injected rats (45% vs. 28%). Re-implantation of the avulsed roots in the injured spinal cord allowed the regeneration of motor axons. By combining root re-implantation and MSC transplant the number of surviving MN at 28 days post-injury was higher (60%) than in re-implantation alone animals (46%). Electromyographic tests showed evidence of functional re-innervation of anterior tibialis and gastrocnemius muscles by the regenerated motor axons only in rats with the combined treatment. These results indicate that MSC are helpful in enhancing neuronal survival and increased the regenerative growth of injured axons. Surgical re-implantation and MSC grafting combined had a synergic neuroprotective effect on MN and on axonal regeneration and muscle re-innervation after spinal root avulsion.

Authors+Show Affiliations

Group of Neuroplasticity and Regeneration, Institute of Neurosciences, Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Bellaterra, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23440700

Citation

Torres-Espín, Abel, et al. "Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion By Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy." Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics, vol. 10, no. 2, 2013, pp. 354-68.
Torres-Espín A, Corona-Quintanilla DL, Forés J, et al. Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy. Neurotherapeutics. 2013;10(2):354-68.
Torres-Espín, A., Corona-Quintanilla, D. L., Forés, J., Allodi, I., González, F., Udina, E., & Navarro, X. (2013). Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy. Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics, 10(2), 354-68. https://doi.org/10.1007/s13311-013-0178-5
Torres-Espín A, et al. Neuroprotection and Axonal Regeneration After Lumbar Ventral Root Avulsion By Re-implantation and Mesenchymal Stem Cells Transplant Combined Therapy. Neurotherapeutics. 2013;10(2):354-68. PubMed PMID: 23440700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy. AU - Torres-Espín,Abel, AU - Corona-Quintanilla,Dora Luz, AU - Forés,Joaquim, AU - Allodi,Ilary, AU - González,Francisco, AU - Udina,Esther, AU - Navarro,Xavier, PY - 2013/2/27/entrez PY - 2013/2/27/pubmed PY - 2013/10/25/medline SP - 354 EP - 68 JF - Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics JO - Neurotherapeutics VL - 10 IS - 2 N2 - Ventral spinal root avulsion causes complete denervation of muscles in the limb and also progressive death of segmental motoneurons (MN) leading to permanent paralysis. The chances for functional recovery after ventral root avulsion are very poor owing to the loss of avulsed neurons and the long distance that surviving neurons have to re-grow axons from the spinal cord to the corresponding targets. Following unilateral avulsion of L4, L5 and L6 spinal roots in adult rats, we performed an intraspinal transplant of mesenchymal stem cells (MSC) and surgical re-implantation of the avulsed roots. Four weeks after avulsion the survival of MN in the MSC-treated animals was significantly higher than in vehicle-injected rats (45% vs. 28%). Re-implantation of the avulsed roots in the injured spinal cord allowed the regeneration of motor axons. By combining root re-implantation and MSC transplant the number of surviving MN at 28 days post-injury was higher (60%) than in re-implantation alone animals (46%). Electromyographic tests showed evidence of functional re-innervation of anterior tibialis and gastrocnemius muscles by the regenerated motor axons only in rats with the combined treatment. These results indicate that MSC are helpful in enhancing neuronal survival and increased the regenerative growth of injured axons. Surgical re-implantation and MSC grafting combined had a synergic neuroprotective effect on MN and on axonal regeneration and muscle re-innervation after spinal root avulsion. SN - 1878-7479 UR - https://www.unboundmedicine.com/medline/citation/23440700/Neuroprotection_and_axonal_regeneration_after_lumbar_ventral_root_avulsion_by_re_implantation_and_mesenchymal_stem_cells_transplant_combined_therapy_ L2 - https://dx.doi.org/10.1007/s13311-013-0178-5 DB - PRIME DP - Unbound Medicine ER -