Tags

Type your tag names separated by a space and hit enter

Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography.
Food Chem. 2012 Oct 15; 134(4):2268-75.FC

Abstract

Green tea seems to have a positive impact on health due to the catechins-found as flavanols. Thus, the present study was aimed to develop a low cost reversed phase high performance liquid chromatographic (HPLC) method for simultaneous determination of flavanol contents, namely catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin 3-gallate (ECG) and epigallocatechin 3-gallate (EGCG) and caffeine in 29 commercial green tea samples available in a Saudi Arabian local market. A C-18 reversed-phase column, acetonitrile-trifluoroacetic acid as a mobile phase, coupled with UV detector at 205 nm, was successfully used for precise analysis of the tested analytes in boiled water of digested tea leaves. The average values of N (No. of theoretical plates), HETP (height equivalent of theoretical plates) and R(s) (separation factor) (at 10 μg ml(-1) of the catechins EC, EGC, EGCG and ECG) were 2.6×10(3)±1.2×10(3), 1.7×10(-3)±4.7×10(-4) cm and 1.7±5.53×10(-2), respectively. The developed HPLC method demonstrated excellent performance, with low limits of detection (LOD) and quantification (LOQ) of the tested catechins of 0.004-0.05 μg ml(-1) and 0.01-0.17 μg ml(-1), respectively, and recovery percentages of 96-101%. The influence of infusion time (5-30 min) and temperature on the content of the flavanols was investigated by HPLC. After a 5 min infusion of the tea leaves, the average concentrations of caffeine, catechin, EC, EGC, ECG and EGCG were found to be in the ranges 0.086-2.23, 0.113-2.94, 0.58-10.22, 0.19-24.9, 0.22-13.9 and 1.01-43.3 mg g(-1), respectively. The contents of caffeine and catechins followed the sequence: EGCG>EGC>ECG>EC>C>caffeine. The method was applied satisfactorily for the analysis of (+)-catechin, even at trace and ultra trace concentrations of catechins. The method was rapid, accurate, reproducible and ideal for routine analysis.

Authors+Show Affiliations

Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia. mohammad_el_shahawi@yahoo.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23442685

Citation

El-Shahawi, M S., et al. "Analysis of some Selected Catechins and Caffeine in Green Tea By High Performance Liquid Chromatography." Food Chemistry, vol. 134, no. 4, 2012, pp. 2268-75.
El-Shahawi MS, Hamza A, Bahaffi SO, et al. Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. Food Chem. 2012;134(4):2268-75.
El-Shahawi, M. S., Hamza, A., Bahaffi, S. O., Al-Sibaai, A. A., & Abduljabbar, T. N. (2012). Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. Food Chemistry, 134(4), 2268-75. https://doi.org/10.1016/j.foodchem.2012.03.039
El-Shahawi MS, et al. Analysis of some Selected Catechins and Caffeine in Green Tea By High Performance Liquid Chromatography. Food Chem. 2012 Oct 15;134(4):2268-75. PubMed PMID: 23442685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. AU - El-Shahawi,M S, AU - Hamza,A, AU - Bahaffi,S O, AU - Al-Sibaai,A A, AU - Abduljabbar,T N, Y1 - 2012/03/28/ PY - 2011/02/06/received PY - 2012/02/16/revised PY - 2012/03/07/accepted PY - 2013/2/28/entrez PY - 2013/2/28/pubmed PY - 2013/8/24/medline SP - 2268 EP - 75 JF - Food chemistry JO - Food Chem VL - 134 IS - 4 N2 - Green tea seems to have a positive impact on health due to the catechins-found as flavanols. Thus, the present study was aimed to develop a low cost reversed phase high performance liquid chromatographic (HPLC) method for simultaneous determination of flavanol contents, namely catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin 3-gallate (ECG) and epigallocatechin 3-gallate (EGCG) and caffeine in 29 commercial green tea samples available in a Saudi Arabian local market. A C-18 reversed-phase column, acetonitrile-trifluoroacetic acid as a mobile phase, coupled with UV detector at 205 nm, was successfully used for precise analysis of the tested analytes in boiled water of digested tea leaves. The average values of N (No. of theoretical plates), HETP (height equivalent of theoretical plates) and R(s) (separation factor) (at 10 μg ml(-1) of the catechins EC, EGC, EGCG and ECG) were 2.6×10(3)±1.2×10(3), 1.7×10(-3)±4.7×10(-4) cm and 1.7±5.53×10(-2), respectively. The developed HPLC method demonstrated excellent performance, with low limits of detection (LOD) and quantification (LOQ) of the tested catechins of 0.004-0.05 μg ml(-1) and 0.01-0.17 μg ml(-1), respectively, and recovery percentages of 96-101%. The influence of infusion time (5-30 min) and temperature on the content of the flavanols was investigated by HPLC. After a 5 min infusion of the tea leaves, the average concentrations of caffeine, catechin, EC, EGC, ECG and EGCG were found to be in the ranges 0.086-2.23, 0.113-2.94, 0.58-10.22, 0.19-24.9, 0.22-13.9 and 1.01-43.3 mg g(-1), respectively. The contents of caffeine and catechins followed the sequence: EGCG>EGC>ECG>EC>C>caffeine. The method was applied satisfactorily for the analysis of (+)-catechin, even at trace and ultra trace concentrations of catechins. The method was rapid, accurate, reproducible and ideal for routine analysis. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23442685/Analysis_of_some_selected_catechins_and_caffeine_in_green_tea_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)00499-2 DB - PRIME DP - Unbound Medicine ER -