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[Nano-Se-chondroitin sulfate inhibits T-2 toxin-induced apoptosis of cultured chondrocytes from patients with Kashin-Beck disease].
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Feb; 33(2):225-9.NF

Abstract

OBJECTIVE

To observe the effect of nano-Se-chondroitin sulfate on the growth and apoptosis of chondrocytes from patients with Kashin-Beck disease (KBD) exposed to T-2 toxin in vitro.

METHODS

Samples of the articular cartilage were obtained from 6 patients with grade II/III KBD diagnosed in line with the National Clinical Diagnostic Criteria of KBD (WS/T 207-2010) for chondrocyte separation and culture in vitro. The separated chondrocytes were treated with synthesized nano-Se-chondroitin sulfate particles and T-2 toxin, alone or in combination, and the cell growth and apoptosis were observed using MTT assay, HE staining and flow cytometry.

RESULTS

The synthesized nano-Se-chondroitin sulfate, with a selenium entrapment ratio of 10.1%, spontaneously formed nanoparticles in distilled water with sizes ranging from 30 to 200 nm. Fourier-transform infrared spectroscopy suggested a possible covalent bond that bound Nano-Se and chondroitin sulfate. Within the concentration range of 50-200 ng/ml, nano-Se-chondroitin sulfate significantly inhibited T-2 toxin-induced apoptosis of the cultured chondrocytes and reduced the early apoptosis rate to (8.64∓1.57)% (P<0.05).

CONCLUSION

Nano-Se-chondroitin sulfate can inhibit T-2 toxin-induced apoptosis of cultured chondrocytes from KBD patients in vitro, and serves as a promising candidate therapeutic agent for KBD.

Authors+Show Affiliations

Xi'an Jiaotong University College of Medicine, Xi'an, China. bbbishop@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23443777

Citation

Han, Jing, et al. "[Nano-Se-chondroitin Sulfate Inhibits T-2 Toxin-induced Apoptosis of Cultured Chondrocytes From Patients With Kashin-Beck Disease]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 33, no. 2, 2013, pp. 225-9.
Han J, Guo X, Wu C, et al. [Nano-Se-chondroitin sulfate inhibits T-2 toxin-induced apoptosis of cultured chondrocytes from patients with Kashin-Beck disease]. Nan Fang Yi Ke Da Xue Xue Bao. 2013;33(2):225-9.
Han, J., Guo, X., Wu, C., Li, C., He, S., Duan, C., & Ning, Y. (2013). [Nano-Se-chondroitin sulfate inhibits T-2 toxin-induced apoptosis of cultured chondrocytes from patients with Kashin-Beck disease]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 33(2), 225-9.
Han J, et al. [Nano-Se-chondroitin Sulfate Inhibits T-2 Toxin-induced Apoptosis of Cultured Chondrocytes From Patients With Kashin-Beck Disease]. Nan Fang Yi Ke Da Xue Xue Bao. 2013;33(2):225-9. PubMed PMID: 23443777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Nano-Se-chondroitin sulfate inhibits T-2 toxin-induced apoptosis of cultured chondrocytes from patients with Kashin-Beck disease]. AU - Han,Jing, AU - Guo,Xiong, AU - Wu,Cuiyan, AU - Li,Chunyan, AU - He,Shulan, AU - Duan,Chen, AU - Ning,Yujie, PY - 2013/2/28/entrez PY - 2013/2/28/pubmed PY - 2014/1/1/medline SP - 225 EP - 9 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 33 IS - 2 N2 - OBJECTIVE: To observe the effect of nano-Se-chondroitin sulfate on the growth and apoptosis of chondrocytes from patients with Kashin-Beck disease (KBD) exposed to T-2 toxin in vitro. METHODS: Samples of the articular cartilage were obtained from 6 patients with grade II/III KBD diagnosed in line with the National Clinical Diagnostic Criteria of KBD (WS/T 207-2010) for chondrocyte separation and culture in vitro. The separated chondrocytes were treated with synthesized nano-Se-chondroitin sulfate particles and T-2 toxin, alone or in combination, and the cell growth and apoptosis were observed using MTT assay, HE staining and flow cytometry. RESULTS: The synthesized nano-Se-chondroitin sulfate, with a selenium entrapment ratio of 10.1%, spontaneously formed nanoparticles in distilled water with sizes ranging from 30 to 200 nm. Fourier-transform infrared spectroscopy suggested a possible covalent bond that bound Nano-Se and chondroitin sulfate. Within the concentration range of 50-200 ng/ml, nano-Se-chondroitin sulfate significantly inhibited T-2 toxin-induced apoptosis of the cultured chondrocytes and reduced the early apoptosis rate to (8.64∓1.57)% (P<0.05). CONCLUSION: Nano-Se-chondroitin sulfate can inhibit T-2 toxin-induced apoptosis of cultured chondrocytes from KBD patients in vitro, and serves as a promising candidate therapeutic agent for KBD. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/23443777/[Nano_Se_chondroitin_sulfate_inhibits_T_2_toxin_induced_apoptosis_of_cultured_chondrocytes_from_patients_with_Kashin_Beck_disease]_ DB - PRIME DP - Unbound Medicine ER -