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Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes.
Arch Virol 2013; 158(7):1477-85AV

Abstract

Orf virus (ORFV) is an enveloped virus with a double-stranded DNA genome, causing a contagious pustular dermatitis, mainly in goats and sheep. In this study, two strains of ORFV were isolated from sheep and goat samples in Xinjiang and Shaanxi, China. The B2L and virus interferon resistance (VIR) genes of these two isolates were sequenced and analyzed after PCR amplification. Phylogenetic analysis showed that the two isolates clustered with other ORFV strains but were separated into different subgroups. The Xinjiang strain shared the highest homology with the Gansu strain, whereas the Shaanxi strain shared higher homology with the Taiwan and Hubei strains. This is the first report of the molecular characterization of ORFV in Northwest China, and it provides new information on the genotyping of the causative agents responsible for contagious ecthyma dermatitis outbreaks in China.

Authors+Show Affiliations

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Xujiaping 1, Yanchangbu, Lanzhou, Gansu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23443934

Citation

Li, Huixia, et al. "Phylogenetic Analysis of Two Chinese Orf Virus Isolates Based On Sequences of B2L and VIR Genes." Archives of Virology, vol. 158, no. 7, 2013, pp. 1477-85.
Li H, Zhu X, Zheng Y, et al. Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes. Arch Virol. 2013;158(7):1477-85.
Li, H., Zhu, X., Zheng, Y., Wang, S., Liu, Z., Dou, Y., ... Luo, X. (2013). Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes. Archives of Virology, 158(7), pp. 1477-85. doi:10.1007/s00705-013-1641-7.
Li H, et al. Phylogenetic Analysis of Two Chinese Orf Virus Isolates Based On Sequences of B2L and VIR Genes. Arch Virol. 2013;158(7):1477-85. PubMed PMID: 23443934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes. AU - Li,Huixia, AU - Zhu,Xueliang, AU - Zheng,Yadong, AU - Wang,Shuai, AU - Liu,Zhenyong, AU - Dou,Yongxi, AU - Li,Hui, AU - Cai,Xuepeng, AU - Luo,Xuenong, Y1 - 2013/02/27/ PY - 2012/09/02/received PY - 2013/01/10/accepted PY - 2013/2/28/entrez PY - 2013/2/28/pubmed PY - 2013/9/5/medline SP - 1477 EP - 85 JF - Archives of virology JO - Arch. Virol. VL - 158 IS - 7 N2 - Orf virus (ORFV) is an enveloped virus with a double-stranded DNA genome, causing a contagious pustular dermatitis, mainly in goats and sheep. In this study, two strains of ORFV were isolated from sheep and goat samples in Xinjiang and Shaanxi, China. The B2L and virus interferon resistance (VIR) genes of these two isolates were sequenced and analyzed after PCR amplification. Phylogenetic analysis showed that the two isolates clustered with other ORFV strains but were separated into different subgroups. The Xinjiang strain shared the highest homology with the Gansu strain, whereas the Shaanxi strain shared higher homology with the Taiwan and Hubei strains. This is the first report of the molecular characterization of ORFV in Northwest China, and it provides new information on the genotyping of the causative agents responsible for contagious ecthyma dermatitis outbreaks in China. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/23443934/Phylogenetic_analysis_of_two_Chinese_orf_virus_isolates_based_on_sequences_of_B2L_and_VIR_genes_ L2 - https://dx.doi.org/10.1007/s00705-013-1641-7 DB - PRIME DP - Unbound Medicine ER -