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Self-incompatibility in Petunia inflata: the relationship between a self-incompatibility locus F-box protein and its non-self S-RNases.
Plant Cell. 2013 Feb; 25(2):470-85.PC

Abstract

The highly polymorphic S (for self-incompatibility) locus regulates self-incompatibility in Petunia inflata; the S-RNase regulates pistil specificity, and multiple S-locus F-box (SLF) genes regulate pollen specificity. The collaborative non-self recognition model predicts that, for any S-haplotype, an unknown number of SLFs collectively recognize all non-self S-RNases to mediate their ubiquitination and degradation. Using a gain-of-function assay, we examined the relationships between S2-SLF1 (for S2-allelic product of Type-1 SLF) and four S-RNases. The results suggest that S2-SLF1 interacts with S7- and S13-RNases, and the previously identified S1- and S3-RNases, but not with S5- or S11-RNase. An artificial microRNA expressed by the S2-SLF1 promoter, but not by the vegetative cell-specific promoter, Late Anther Tomato 52, suppressed expression of S2-SLF1 in S2 pollen, suggesting that SLF1 is specific to the generative cell. The S2 pollen with S2-SLF1 suppressed was compatible with S3-, S5-, S7-, S11-, and S13-carrying pistils, confirming that other SLF proteins are responsible for detoxifying S5- and S11-RNases and suggesting that S2-SLF1 is not the only SLF in S2 pollen that interacts with S3-, S7-, and S13-RNases. Petunia may have evolved at least two types of SLF proteins to detoxify any non-self S-RNase to minimize the deleterious effects of mutation in any SLF.

Authors+Show Affiliations

Intercollege Graduate Degree Program in Plant Biology, Pensylvania State University, University Park, PA 16802, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23444333

Citation

Sun, Penglin, and Teh-hui Kao. "Self-incompatibility in Petunia Inflata: the Relationship Between a Self-incompatibility Locus F-box Protein and Its Non-self S-RNases." The Plant Cell, vol. 25, no. 2, 2013, pp. 470-85.
Sun P, Kao TH. Self-incompatibility in Petunia inflata: the relationship between a self-incompatibility locus F-box protein and its non-self S-RNases. Plant Cell. 2013;25(2):470-85.
Sun, P., & Kao, T. H. (2013). Self-incompatibility in Petunia inflata: the relationship between a self-incompatibility locus F-box protein and its non-self S-RNases. The Plant Cell, 25(2), 470-85. https://doi.org/10.1105/tpc.112.106294
Sun P, Kao TH. Self-incompatibility in Petunia Inflata: the Relationship Between a Self-incompatibility Locus F-box Protein and Its Non-self S-RNases. Plant Cell. 2013;25(2):470-85. PubMed PMID: 23444333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Self-incompatibility in Petunia inflata: the relationship between a self-incompatibility locus F-box protein and its non-self S-RNases. AU - Sun,Penglin, AU - Kao,Teh-hui, Y1 - 2013/02/26/ PY - 2013/2/28/entrez PY - 2013/2/28/pubmed PY - 2014/1/16/medline SP - 470 EP - 85 JF - The Plant cell JO - Plant Cell VL - 25 IS - 2 N2 - The highly polymorphic S (for self-incompatibility) locus regulates self-incompatibility in Petunia inflata; the S-RNase regulates pistil specificity, and multiple S-locus F-box (SLF) genes regulate pollen specificity. The collaborative non-self recognition model predicts that, for any S-haplotype, an unknown number of SLFs collectively recognize all non-self S-RNases to mediate their ubiquitination and degradation. Using a gain-of-function assay, we examined the relationships between S2-SLF1 (for S2-allelic product of Type-1 SLF) and four S-RNases. The results suggest that S2-SLF1 interacts with S7- and S13-RNases, and the previously identified S1- and S3-RNases, but not with S5- or S11-RNase. An artificial microRNA expressed by the S2-SLF1 promoter, but not by the vegetative cell-specific promoter, Late Anther Tomato 52, suppressed expression of S2-SLF1 in S2 pollen, suggesting that SLF1 is specific to the generative cell. The S2 pollen with S2-SLF1 suppressed was compatible with S3-, S5-, S7-, S11-, and S13-carrying pistils, confirming that other SLF proteins are responsible for detoxifying S5- and S11-RNases and suggesting that S2-SLF1 is not the only SLF in S2 pollen that interacts with S3-, S7-, and S13-RNases. Petunia may have evolved at least two types of SLF proteins to detoxify any non-self S-RNase to minimize the deleterious effects of mutation in any SLF. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23444333/Self_incompatibility_in_Petunia_inflata:_the_relationship_between_a_self_incompatibility_locus_F_box_protein_and_its_non_self_S_RNases_ L2 - https://academic.oup.com/plcell/article-lookup/doi/10.1105/tpc.112.106294 DB - PRIME DP - Unbound Medicine ER -