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Citron kinase mediates transition from constriction to abscission through its coiled-coil domain.
J Cell Sci. 2013 Apr 15; 126(Pt 8):1773-84.JC

Abstract

Cytokinesis is initiated by constriction of the cleavage furrow, and completed with separation of the two daughter cells by abscission. Control of transition from constriction to abscission is therefore crucial for cytokinesis. However, the underlying mechanism is largely unknown. Here, we analyze the role of Citron kinase (Citron-K) that localizes at the cleavage furrow and the midbody, and dissect its action mechanisms during this transition. Citron-K forms a stable ring-like structure at the midbody and its depletion affects the maintenance of the intercellular bridge, resulting in fusion of two daughter cells after the cleavage furrow ingression. RNA interference (RNAi) targeting Citron-K reduced accumulation of RhoA, Anillin, and septins at the intercellular bridge in mid telophase, and impaired concentration and maintenance of KIF14 and PRC1 at the midbody in late telophase. RNAi rescue experiments revealed that these functions of Citron-K are mediated by its coiled-coil (CC) domain, and not by its kinase domain. The C-terminal part of CC contains a Rho-binding domain and a cluster-forming region and is important for concentrating Citron-K from the cleavage furrow to the midbody. The N-terminal part of CC directly binds to KIF14, and this interaction is required for timely transfer of Citron-K to the midbody after furrow ingression. We propose that the CC-domain-mediated translocation and actions of Citron-K ensure proper stabilization of the midbody structure during the transition from constriction to abscission.

Authors+Show Affiliations

Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606-8501, Japan. sadanori.watanabe@bio.nagoya-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23444367

Citation

Watanabe, Sadanori, et al. "Citron Kinase Mediates Transition From Constriction to Abscission Through Its Coiled-coil Domain." Journal of Cell Science, vol. 126, no. Pt 8, 2013, pp. 1773-84.
Watanabe S, De Zan T, Ishizaki T, et al. Citron kinase mediates transition from constriction to abscission through its coiled-coil domain. J Cell Sci. 2013;126(Pt 8):1773-84.
Watanabe, S., De Zan, T., Ishizaki, T., & Narumiya, S. (2013). Citron kinase mediates transition from constriction to abscission through its coiled-coil domain. Journal of Cell Science, 126(Pt 8), 1773-84. https://doi.org/10.1242/jcs.116608
Watanabe S, et al. Citron Kinase Mediates Transition From Constriction to Abscission Through Its Coiled-coil Domain. J Cell Sci. 2013 Apr 15;126(Pt 8):1773-84. PubMed PMID: 23444367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Citron kinase mediates transition from constriction to abscission through its coiled-coil domain. AU - Watanabe,Sadanori, AU - De Zan,Tihana, AU - Ishizaki,Toshimasa, AU - Narumiya,Shuh, Y1 - 2013/02/26/ PY - 2013/2/28/entrez PY - 2013/2/28/pubmed PY - 2013/10/30/medline KW - Abscission KW - Cytokinesis KW - Midbody SP - 1773 EP - 84 JF - Journal of cell science JO - J. Cell. Sci. VL - 126 IS - Pt 8 N2 - Cytokinesis is initiated by constriction of the cleavage furrow, and completed with separation of the two daughter cells by abscission. Control of transition from constriction to abscission is therefore crucial for cytokinesis. However, the underlying mechanism is largely unknown. Here, we analyze the role of Citron kinase (Citron-K) that localizes at the cleavage furrow and the midbody, and dissect its action mechanisms during this transition. Citron-K forms a stable ring-like structure at the midbody and its depletion affects the maintenance of the intercellular bridge, resulting in fusion of two daughter cells after the cleavage furrow ingression. RNA interference (RNAi) targeting Citron-K reduced accumulation of RhoA, Anillin, and septins at the intercellular bridge in mid telophase, and impaired concentration and maintenance of KIF14 and PRC1 at the midbody in late telophase. RNAi rescue experiments revealed that these functions of Citron-K are mediated by its coiled-coil (CC) domain, and not by its kinase domain. The C-terminal part of CC contains a Rho-binding domain and a cluster-forming region and is important for concentrating Citron-K from the cleavage furrow to the midbody. The N-terminal part of CC directly binds to KIF14, and this interaction is required for timely transfer of Citron-K to the midbody after furrow ingression. We propose that the CC-domain-mediated translocation and actions of Citron-K ensure proper stabilization of the midbody structure during the transition from constriction to abscission. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/23444367/Citron_kinase_mediates_transition_from_constriction_to_abscission_through_its_coiled_coil_domain_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=23444367 DB - PRIME DP - Unbound Medicine ER -