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Genetic combination of angiotensin-converting enzyme with methylene tetrahydrofolate reductase polymorphisms and the risk of type 2 diabetes mellitus in Bahrain.

Abstract

INTRODUCTION

Bahrain has a high prevalence of type 2 diabetes mellitus (T2DM). Previously, Angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism was found to be associated with T2DM in Bahrainis. The relationship between the disease progression in Bahraini T2DM population and the genetic polymorphism of methylene-tetrahydrofolate-reductase (MTHFR) C677T is still under investigation.

AIM

The current study investigated the distribution of MTHFR C677T gene polymorphism among Bahraini T2DM patients and examined the interaction between ACE I/D and MTHFR C677T polymorphisms on the risk of developing T2DM and its long-term complications.

MATERIALS AND METHODS

Polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) were used to test for the presence of ACE I/D and MTHFR C677T polymorphisms in 171 patients with T2DM compared to 188 healthy (non-diabetic) age-matched control subjects from Bahrain.

RESULTS

The incidence of the DD genotype and D allele of the ACE gene was high among Bahraini T2DM patients. MTHFR allele and genotype frequencies did not differ between patients and controls. No significant relationship was identified between the combinations of ACE I/D and MTHFR C677T polymorphisms with T2DM.

CONCLUSIONS

The results clearly showed an association of the ACE I/D polymorphism with the progression of T2DM, but when it interacts with MTHFR polymorphism no influence was detected on the increased risk of T2DM.

Authors+Show Affiliations

Molecular Genetics Laboratory, Kuwait Medical Genetic Center, State of Kuwait alharbi_em@yahoo.com.College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of Bahrain.College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of Bahrain.Department of Pathology, Bahrain Defense Force Royal Medical Services Hospital, Kingdom of Bahrain.General Department of Criminal Evidences, Ministry of Interior, Farwaneyia, State of Kuwait.School of Forensic and Investigative Sciences, University of Central Lancashire, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23447104

Citation

Al-Harbi, Einas M., et al. "Genetic Combination of Angiotensin-converting Enzyme With Methylene Tetrahydrofolate Reductase Polymorphisms and the Risk of Type 2 Diabetes Mellitus in Bahrain." Journal of the Renin-angiotensin-aldosterone System : JRAAS, vol. 16, no. 1, 2015, pp. 172-7.
Al-Harbi EM, Farid EM, Gumaa KA, et al. Genetic combination of angiotensin-converting enzyme with methylene tetrahydrofolate reductase polymorphisms and the risk of type 2 diabetes mellitus in Bahrain. J Renin Angiotensin Aldosterone Syst. 2015;16(1):172-7.
Al-Harbi, E. M., Farid, E. M., Gumaa, K. A., Darwish, A. H., Alenizi, M., & Singh, J. (2015). Genetic combination of angiotensin-converting enzyme with methylene tetrahydrofolate reductase polymorphisms and the risk of type 2 diabetes mellitus in Bahrain. Journal of the Renin-angiotensin-aldosterone System : JRAAS, 16(1), pp. 172-7. doi:10.1177/1470320313478286.
Al-Harbi EM, et al. Genetic Combination of Angiotensin-converting Enzyme With Methylene Tetrahydrofolate Reductase Polymorphisms and the Risk of Type 2 Diabetes Mellitus in Bahrain. J Renin Angiotensin Aldosterone Syst. 2015;16(1):172-7. PubMed PMID: 23447104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic combination of angiotensin-converting enzyme with methylene tetrahydrofolate reductase polymorphisms and the risk of type 2 diabetes mellitus in Bahrain. AU - Al-Harbi,Einas M, AU - Farid,Eman M, AU - Gumaa,Khalid A, AU - Darwish,Abdulla H, AU - Alenizi,Mohammad, AU - Singh,Jaipaul, Y1 - 2013/02/27/ PY - 2014/09/14/received PY - 2014/09/28/accepted PY - 2013/3/1/entrez PY - 2013/3/1/pubmed PY - 2016/2/2/medline KW - Bahrain KW - angiotensin-converting enzyme KW - methylene-tetrahydrofolate-reductase KW - polymorphism KW - type 2 diabetes mellitus SP - 172 EP - 7 JF - Journal of the renin-angiotensin-aldosterone system : JRAAS JO - J Renin Angiotensin Aldosterone Syst VL - 16 IS - 1 N2 - INTRODUCTION: Bahrain has a high prevalence of type 2 diabetes mellitus (T2DM). Previously, Angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism was found to be associated with T2DM in Bahrainis. The relationship between the disease progression in Bahraini T2DM population and the genetic polymorphism of methylene-tetrahydrofolate-reductase (MTHFR) C677T is still under investigation. AIM: The current study investigated the distribution of MTHFR C677T gene polymorphism among Bahraini T2DM patients and examined the interaction between ACE I/D and MTHFR C677T polymorphisms on the risk of developing T2DM and its long-term complications. MATERIALS AND METHODS: Polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) were used to test for the presence of ACE I/D and MTHFR C677T polymorphisms in 171 patients with T2DM compared to 188 healthy (non-diabetic) age-matched control subjects from Bahrain. RESULTS: The incidence of the DD genotype and D allele of the ACE gene was high among Bahraini T2DM patients. MTHFR allele and genotype frequencies did not differ between patients and controls. No significant relationship was identified between the combinations of ACE I/D and MTHFR C677T polymorphisms with T2DM. CONCLUSIONS: The results clearly showed an association of the ACE I/D polymorphism with the progression of T2DM, but when it interacts with MTHFR polymorphism no influence was detected on the increased risk of T2DM. SN - 1752-8976 UR - https://www.unboundmedicine.com/medline/citation/23447104/Genetic_combination_of_angiotensin_converting_enzyme_with_methylene_tetrahydrofolate_reductase_polymorphisms_and_the_risk_of_type_2_diabetes_mellitus_in_Bahrain_ L2 - http://journals.sagepub.com/doi/full/10.1177/1470320313478286?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -