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In vitro activity-guided identification of antioxidants in aged garlic extract.
J Agric Food Chem. 2013 Mar 27; 61(12):3059-67.JA

Abstract

Activity-guided fractionation was applied on an aged garlic extract (AGE), reported to show strong antioxidant activity, in order to locate the key in vitro antioxidant ingredients by means of the hydrogen peroxide scavenging (HPS) assay as well as the ORAC assay. Besides the previously reported four tetrahydro-β-carbolines, (1R,3S)- and (1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and (1R,3S)- and (1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-1,3-dicarboxylic acid, LC-MS/MS, LC-TOF-MS, and 1D/2D-NMR experiments led to the identification of coniferyl alcohol and its dilignols (-)-(2R,3S)-dihydrodehydrodiconiferyl alcohol, (+)-(2S,3R)-dehydrodiconiferyl alcohol, erythro-guaiacylglycerol-β-O-4'-coniferyl ether, and threo-guaiacylglycerol-β-O-4'-coniferyl ether as the major antioxidants in AGE. The purified individual compounds showed high antioxidant activity, with EC50 values of 9.7-11.8 μM (HPS assay) and 2.60-3.65 μmol TE/μmol (ORAC assay), respectively.

Authors+Show Affiliations

Technische Universität München , Lise-Meitner Strasse 34, D-85354 Freising, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23448127

Citation

Matsutomo, Toshiaki, et al. "In Vitro Activity-guided Identification of Antioxidants in Aged Garlic Extract." Journal of Agricultural and Food Chemistry, vol. 61, no. 12, 2013, pp. 3059-67.
Matsutomo T, Stark TD, Hofmann T. In vitro activity-guided identification of antioxidants in aged garlic extract. J Agric Food Chem. 2013;61(12):3059-67.
Matsutomo, T., Stark, T. D., & Hofmann, T. (2013). In vitro activity-guided identification of antioxidants in aged garlic extract. Journal of Agricultural and Food Chemistry, 61(12), 3059-67. https://doi.org/10.1021/jf305549g
Matsutomo T, Stark TD, Hofmann T. In Vitro Activity-guided Identification of Antioxidants in Aged Garlic Extract. J Agric Food Chem. 2013 Mar 27;61(12):3059-67. PubMed PMID: 23448127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity-guided identification of antioxidants in aged garlic extract. AU - Matsutomo,Toshiaki, AU - Stark,Timo D, AU - Hofmann,Thomas, Y1 - 2013/03/12/ PY - 2013/3/2/entrez PY - 2013/3/2/pubmed PY - 2013/12/16/medline SP - 3059 EP - 67 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 61 IS - 12 N2 - Activity-guided fractionation was applied on an aged garlic extract (AGE), reported to show strong antioxidant activity, in order to locate the key in vitro antioxidant ingredients by means of the hydrogen peroxide scavenging (HPS) assay as well as the ORAC assay. Besides the previously reported four tetrahydro-β-carbolines, (1R,3S)- and (1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and (1R,3S)- and (1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-1,3-dicarboxylic acid, LC-MS/MS, LC-TOF-MS, and 1D/2D-NMR experiments led to the identification of coniferyl alcohol and its dilignols (-)-(2R,3S)-dihydrodehydrodiconiferyl alcohol, (+)-(2S,3R)-dehydrodiconiferyl alcohol, erythro-guaiacylglycerol-β-O-4'-coniferyl ether, and threo-guaiacylglycerol-β-O-4'-coniferyl ether as the major antioxidants in AGE. The purified individual compounds showed high antioxidant activity, with EC50 values of 9.7-11.8 μM (HPS assay) and 2.60-3.65 μmol TE/μmol (ORAC assay), respectively. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/23448127/In_vitro_activity_guided_identification_of_antioxidants_in_aged_garlic_extract_ L2 - https://doi.org/10.1021/jf305549g DB - PRIME DP - Unbound Medicine ER -