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Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis.
FEBS Lett. 2013 Apr 02; 587(7):936-42.FL

Abstract

Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.

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Authors+Show Affiliations

Tjellström H
Department of Plant Biology, Great Lakes Bioenergy Research Center Michigan State University, East Lansing, MI 48824, USA. henrik@msu.edu
Strawsine M
No affiliation info available
Silva J
No affiliation info available
Cahoon EB
No affiliation info available
Ohlrogge JB
No affiliation info available

MeSH

ArabidopsisArabidopsis ProteinsCarbon-Sulfur LigasesCupheaFatty AcidsMutationPlants, Genetically ModifiedPlastidsSeedsSpectrometry, Mass, Electrospray IonizationThiolester HydrolasesTriglycerides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23454211

Citation

Tjellström, Henrik, et al. "Disruption of Plastid Acyl:acyl Carrier Protein Synthetases Increases Medium Chain Fatty Acid Accumulation in Seeds of Transgenic Arabidopsis." FEBS Letters, vol. 587, no. 7, 2013, pp. 936-42.
Tjellström H, Strawsine M, Silva J, et al. Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis. FEBS Lett. 2013;587(7):936-42.
Tjellström, H., Strawsine, M., Silva, J., Cahoon, E. B., & Ohlrogge, J. B. (2013). Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis. FEBS Letters, 587(7), 936-42. https://doi.org/10.1016/j.febslet.2013.02.021
Tjellström H, et al. Disruption of Plastid Acyl:acyl Carrier Protein Synthetases Increases Medium Chain Fatty Acid Accumulation in Seeds of Transgenic Arabidopsis. FEBS Lett. 2013 Apr 2;587(7):936-42. PubMed PMID: 23454211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis. AU - Tjellström,Henrik, AU - Strawsine,Merissa, AU - Silva,Jillian, AU - Cahoon,Edgar B, AU - Ohlrogge,John B, Y1 - 2013/02/20/ PY - 2012/11/29/received PY - 2013/01/17/revised PY - 2013/02/08/accepted PY - 2013/3/5/entrez PY - 2013/3/5/pubmed PY - 2013/5/28/medline SP - 936 EP - 42 JF - FEBS letters JO - FEBS Lett VL - 587 IS - 7 N2 - Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/23454211/Disruption_of_plastid_acyl:acyl_carrier_protein_synthetases_increases_medium_chain_fatty_acid_accumulation_in_seeds_of_transgenic_Arabidopsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(13)00152-X DB - PRIME DP - Unbound Medicine ER -
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