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In vitro activity of cadazolid against Clostridium difficile strains isolated from primary and recurrent infections in Stockholm, Sweden.
Anaerobe. 2013 Apr; 20:32-5.A

Abstract

One hundred thirty-three Clostridium difficile strains were collected from 71 patients and analyzed for the presence of C. difficile toxin B by the cell cytotoxicity neutralization assay, genes for toxin A, toxin B, binary toxin and TcdC deletion by PCR. All strains were also PCR-ribotyped and analyzed for sporulation frequency. The MICs of the isolates were determined against cadazolid and seven other antimicrobial agents by the agar dilution method. All isolates were positive for toxin B by the cell cytotoxicity neutralization assay. One hundred fourteen isolates were positive for toxin A and B and 16 isolates were positive for toxin A, toxin B and binary toxin by PCR. Three isolates were negative for toxin A but positive for toxin B. Thirty-three different ribotypes were identified. No strain of ribotype 027 was found. No differences in sporulation were noticed between the primary and recurrent isolates. All 133 isolates were sensitive to cadazolid (0.064-0.5 mg/l), fidaxomicin (0.008-0.125 mg/l), metronidazole (0.125-2 mg/l), vancomycin (0.125-1 mg/l) and tigecycline (0.032-0.25 mg/l). Three isolates were resistant to linezolid (8 mg/l), 15 isolates were resistant to moxifloxacin (8-32 mg/l) and 103 isolates were resistant to clindamycin (8-256 mg/l). No association between toxins A, B and binary toxin, ribotypes or the sporulation and the sensitivity to cadazolid could be found. Cadazolid has a potent in vitro activity against C. difficile.

Authors+Show Affiliations

Department of Laboratory Medicine, Karolinska University Hospital, Karolinska Institutet, SE-141 86 Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23454525

Citation

Rashid, Mamun-Ur, et al. "In Vitro Activity of Cadazolid Against Clostridium Difficile Strains Isolated From Primary and Recurrent Infections in Stockholm, Sweden." Anaerobe, vol. 20, 2013, pp. 32-5.
Rashid MU, Lozano HM, Weintraub A, et al. In vitro activity of cadazolid against Clostridium difficile strains isolated from primary and recurrent infections in Stockholm, Sweden. Anaerobe. 2013;20:32-5.
Rashid, M. U., Lozano, H. M., Weintraub, A., & Nord, C. E. (2013). In vitro activity of cadazolid against Clostridium difficile strains isolated from primary and recurrent infections in Stockholm, Sweden. Anaerobe, 20, 32-5. https://doi.org/10.1016/j.anaerobe.2013.02.003
Rashid MU, et al. In Vitro Activity of Cadazolid Against Clostridium Difficile Strains Isolated From Primary and Recurrent Infections in Stockholm, Sweden. Anaerobe. 2013;20:32-5. PubMed PMID: 23454525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity of cadazolid against Clostridium difficile strains isolated from primary and recurrent infections in Stockholm, Sweden. AU - Rashid,Mamun-Ur, AU - Lozano,Helena Martinez, AU - Weintraub,Andrej, AU - Nord,Carl Erik, Y1 - 2013/02/26/ PY - 2012/12/20/received PY - 2013/02/12/revised PY - 2013/02/19/accepted PY - 2013/3/5/entrez PY - 2013/3/5/pubmed PY - 2013/9/17/medline SP - 32 EP - 5 JF - Anaerobe JO - Anaerobe VL - 20 N2 - One hundred thirty-three Clostridium difficile strains were collected from 71 patients and analyzed for the presence of C. difficile toxin B by the cell cytotoxicity neutralization assay, genes for toxin A, toxin B, binary toxin and TcdC deletion by PCR. All strains were also PCR-ribotyped and analyzed for sporulation frequency. The MICs of the isolates were determined against cadazolid and seven other antimicrobial agents by the agar dilution method. All isolates were positive for toxin B by the cell cytotoxicity neutralization assay. One hundred fourteen isolates were positive for toxin A and B and 16 isolates were positive for toxin A, toxin B and binary toxin by PCR. Three isolates were negative for toxin A but positive for toxin B. Thirty-three different ribotypes were identified. No strain of ribotype 027 was found. No differences in sporulation were noticed between the primary and recurrent isolates. All 133 isolates were sensitive to cadazolid (0.064-0.5 mg/l), fidaxomicin (0.008-0.125 mg/l), metronidazole (0.125-2 mg/l), vancomycin (0.125-1 mg/l) and tigecycline (0.032-0.25 mg/l). Three isolates were resistant to linezolid (8 mg/l), 15 isolates were resistant to moxifloxacin (8-32 mg/l) and 103 isolates were resistant to clindamycin (8-256 mg/l). No association between toxins A, B and binary toxin, ribotypes or the sporulation and the sensitivity to cadazolid could be found. Cadazolid has a potent in vitro activity against C. difficile. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/23454525/In_vitro_activity_of_cadazolid_against_Clostridium_difficile_strains_isolated_from_primary_and_recurrent_infections_in_Stockholm_Sweden_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(13)00023-1 DB - PRIME DP - Unbound Medicine ER -