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In house development of (99m)Tc-Rhenium sulfide colloidal nanoparticles for sentinel lymph node detection.
Pak J Pharm Sci. 2013 Mar; 26(2):367-73.PJ

Abstract

In this study, rhenium sulfide colloidal nanoparticles were developed as radiopharmaceutical for sentinel lymph node detection. We directly used rhenium sulfide as a starting material for the preparation of colloidal nanoparticles. UV-visible spectrophotometry was used for characterization of in house developed colloidal particles. The size distribution of radioactive particles was studied by using membrane filtration method. The percentage of radiolabeled colloidal nanoparticles was determined by paper chromatography (PC). The study also includes in vitro stability, protein binding in human blood and bioevaluation in a rabbit model. The results indicate that 77.27 ± 3.26 % particles of size less than 20nm (suitable for lymphoscintigraphy) were radiolabeled. (99m)Tc labeled rhenium sulfide labeling efficacy with the radiometal is 98.5 ± 0.5%, which remains considerably stable beyond 5h at room temperature. Furthermore, it was observed that 70.2 ± 1.3% radiolabeled colloid complex showed binding with the blood protein. Bioevaluation results show the remarkable achievement of our radiopharmaceutical. The in house prepared (99m)Tc labeled rhenium sulfide colloidal nanoparticles reached the sentinel node within 15 min of post injection. These results indicate that (99m)Tc labeled rhenium sulfide colloid nanoparticles kit produced by a novel procedure seems of significant potential as a feasible candidate for further development to be used in clinical practice.

Authors+Show Affiliations

Dar UK
Institute of Nuclear Medicine & Oncology, Lahore, Pakistan.
Khan I
No affiliation info available
Javed M
No affiliation info available
Ali M
No affiliation info available
Hyder SW
No affiliation info available
Murad S
No affiliation info available
Anwar J
No affiliation info available

MeSH

AnimalsDrug StabilityHumansInjections, IntradermalIsotope LabelingLymph NodesLymphoscintigraphyNanoparticlesParticle SizeProtein BindingRabbitsRadiopharmaceuticalsRheniumSpectrophotometry, UltravioletTechnetium Tc 99m Sulfur ColloidTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23455209

Citation

Dar, Ume-Kalsoom, et al. "In House Development of (99m)Tc-Rhenium Sulfide Colloidal Nanoparticles for Sentinel Lymph Node Detection." Pakistan Journal of Pharmaceutical Sciences, vol. 26, no. 2, 2013, pp. 367-73.
Dar UK, Khan I, Javed M, et al. In house development of (99m)Tc-Rhenium sulfide colloidal nanoparticles for sentinel lymph node detection. Pak J Pharm Sci. 2013;26(2):367-73.
Dar, U. K., Khan, I., Javed, M., Ali, M., Hyder, S. W., Murad, S., & Anwar, J. (2013). In house development of (99m)Tc-Rhenium sulfide colloidal nanoparticles for sentinel lymph node detection. Pakistan Journal of Pharmaceutical Sciences, 26(2), 367-73.
Dar UK, et al. In House Development of (99m)Tc-Rhenium Sulfide Colloidal Nanoparticles for Sentinel Lymph Node Detection. Pak J Pharm Sci. 2013;26(2):367-73. PubMed PMID: 23455209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In house development of (99m)Tc-Rhenium sulfide colloidal nanoparticles for sentinel lymph node detection. AU - Dar,Ume-Kalsoom, AU - Khan,Irfanullah, AU - Javed,Muhammad, AU - Ali,Muhammad, AU - Hyder,Syed Waqar, AU - Murad,Sohail, AU - Anwar,Jamil, PY - 2013/3/5/entrez PY - 2013/3/5/pubmed PY - 2013/6/19/medline SP - 367 EP - 73 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 26 IS - 2 N2 - In this study, rhenium sulfide colloidal nanoparticles were developed as radiopharmaceutical for sentinel lymph node detection. We directly used rhenium sulfide as a starting material for the preparation of colloidal nanoparticles. UV-visible spectrophotometry was used for characterization of in house developed colloidal particles. The size distribution of radioactive particles was studied by using membrane filtration method. The percentage of radiolabeled colloidal nanoparticles was determined by paper chromatography (PC). The study also includes in vitro stability, protein binding in human blood and bioevaluation in a rabbit model. The results indicate that 77.27 ± 3.26 % particles of size less than 20nm (suitable for lymphoscintigraphy) were radiolabeled. (99m)Tc labeled rhenium sulfide labeling efficacy with the radiometal is 98.5 ± 0.5%, which remains considerably stable beyond 5h at room temperature. Furthermore, it was observed that 70.2 ± 1.3% radiolabeled colloid complex showed binding with the blood protein. Bioevaluation results show the remarkable achievement of our radiopharmaceutical. The in house prepared (99m)Tc labeled rhenium sulfide colloidal nanoparticles reached the sentinel node within 15 min of post injection. These results indicate that (99m)Tc labeled rhenium sulfide colloid nanoparticles kit produced by a novel procedure seems of significant potential as a feasible candidate for further development to be used in clinical practice. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/23455209/In_house_development_of__99m_Tc_Rhenium_sulfide_colloidal_nanoparticles_for_sentinel_lymph_node_detection_ DB - PRIME DP - Unbound Medicine ER -