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Searching for viral antibodies and genome in intraocular fluids of patients with Fuchs uveitis and non-infectious uveitis.
Graefes Arch Clin Exp Ophthalmol. 2013 Jun; 251(6):1607-12.GA

Abstract

BACKGROUND

To characterise the polyspecific intraocular antibody synthesis in aqueous humor of patients with Fuchs uveitis and other types of non-infectious uveitis.

METHODS

Aqueous and serum samples collected from 24 patients with Fuchs uveitis, 21 patients with non-infectious uveitis, and 27 healthy subjects undergoing elective cataract surgery (control group) were analysed. In addition, vitreous samples, collected from seven uveitis patients (five Fuchs and two panuveitis) during retinal surgery, were examined. Specific immunoglobulin G antibodies against cytomegalovirus (CMV), rubella virus, herpes simplex virus (HSV), and varicella zoster virus (VZV) were investigated, and Goldmann-Witmer coefficients (GWCs) were calculated. Real-time PCR was performed to detect viral genome for HSV, VZV, and CMV, while nested PCR was conducted to detect rubella RNA.

RESULTS

None of the control samples tested positive for any of the viral antibodies investigated. Intraocular antibody production was found in eight samples of patients affected by Fuchs uveitis (6/8 positive for rubella virus and 2/8 positive for herpes virus). Among patients with non-infectious uveitis, three tested positive for intraocular antibody production (one RV, one HSV and one for VZV). PCR was positive for RV in two patients with Fuchs uveitis, in three patients with non-infectious uveitis (one for RV and two for HSV), and in three control subjects (one for CMV and one for HSV).

CONCLUSIONS

Our series confirmed the presence of specific viral antibodies, especially against rubella virus, in the subgroup of patients affected by Fuchs uveitis, suggesting that this virus may be responsible for this chronic inflammatory condition. Rubella virus is probably the main causative agent of Fuchs uveitis, but other viruses may also be involved in the pathogenesis of this disease.

Authors+Show Affiliations

Eye Unit, Arcispedale Santa Maria Nuova - IRCCS, Viale Risorgimento 80, 42123, Reggio Emilia, Italy. l.cimino64@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23456172

Citation

Cimino, Luca, et al. "Searching for Viral Antibodies and Genome in Intraocular Fluids of Patients With Fuchs Uveitis and Non-infectious Uveitis." Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, vol. 251, no. 6, 2013, pp. 1607-12.
Cimino L, Aldigeri R, Parmeggiani M, et al. Searching for viral antibodies and genome in intraocular fluids of patients with Fuchs uveitis and non-infectious uveitis. Graefes Arch Clin Exp Ophthalmol. 2013;251(6):1607-12.
Cimino, L., Aldigeri, R., Parmeggiani, M., Belloni, L., Zotti, C. A., Fontana, L., Invernizzi, A., Salvarani, C., & Cappuccini, L. (2013). Searching for viral antibodies and genome in intraocular fluids of patients with Fuchs uveitis and non-infectious uveitis. Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 251(6), 1607-12. https://doi.org/10.1007/s00417-013-2287-6
Cimino L, et al. Searching for Viral Antibodies and Genome in Intraocular Fluids of Patients With Fuchs Uveitis and Non-infectious Uveitis. Graefes Arch Clin Exp Ophthalmol. 2013;251(6):1607-12. PubMed PMID: 23456172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Searching for viral antibodies and genome in intraocular fluids of patients with Fuchs uveitis and non-infectious uveitis. AU - Cimino,Luca, AU - Aldigeri,Raffaella, AU - Parmeggiani,Maria, AU - Belloni,Lucia, AU - Zotti,Carlo Alberto, AU - Fontana,Luigi, AU - Invernizzi,Alessandro, AU - Salvarani,Carlo, AU - Cappuccini,Luca, Y1 - 2013/03/03/ PY - 2012/07/31/received PY - 2013/02/12/accepted PY - 2013/01/11/revised PY - 2013/3/5/entrez PY - 2013/3/5/pubmed PY - 2013/11/7/medline SP - 1607 EP - 12 JF - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JO - Graefes Arch. Clin. Exp. Ophthalmol. VL - 251 IS - 6 N2 - BACKGROUND: To characterise the polyspecific intraocular antibody synthesis in aqueous humor of patients with Fuchs uveitis and other types of non-infectious uveitis. METHODS: Aqueous and serum samples collected from 24 patients with Fuchs uveitis, 21 patients with non-infectious uveitis, and 27 healthy subjects undergoing elective cataract surgery (control group) were analysed. In addition, vitreous samples, collected from seven uveitis patients (five Fuchs and two panuveitis) during retinal surgery, were examined. Specific immunoglobulin G antibodies against cytomegalovirus (CMV), rubella virus, herpes simplex virus (HSV), and varicella zoster virus (VZV) were investigated, and Goldmann-Witmer coefficients (GWCs) were calculated. Real-time PCR was performed to detect viral genome for HSV, VZV, and CMV, while nested PCR was conducted to detect rubella RNA. RESULTS: None of the control samples tested positive for any of the viral antibodies investigated. Intraocular antibody production was found in eight samples of patients affected by Fuchs uveitis (6/8 positive for rubella virus and 2/8 positive for herpes virus). Among patients with non-infectious uveitis, three tested positive for intraocular antibody production (one RV, one HSV and one for VZV). PCR was positive for RV in two patients with Fuchs uveitis, in three patients with non-infectious uveitis (one for RV and two for HSV), and in three control subjects (one for CMV and one for HSV). CONCLUSIONS: Our series confirmed the presence of specific viral antibodies, especially against rubella virus, in the subgroup of patients affected by Fuchs uveitis, suggesting that this virus may be responsible for this chronic inflammatory condition. Rubella virus is probably the main causative agent of Fuchs uveitis, but other viruses may also be involved in the pathogenesis of this disease. SN - 1435-702X UR - https://www.unboundmedicine.com/medline/citation/23456172/Searching_for_viral_antibodies_and_genome_in_intraocular_fluids_of_patients_with_Fuchs_uveitis_and_non_infectious_uveitis_ L2 - https://dx.doi.org/10.1007/s00417-013-2287-6 DB - PRIME DP - Unbound Medicine ER -