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Antioxidant activity and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace.
J Sci Food Agric. 2013 Aug 15; 93(10):2502-6.JS

Abstract

BACKGROUND

Phenolic compounds are the predominant ingredients in apple pomace. However, polyphenols from industrial apple pomace, which usually consists of several cultivars, have not been studied in detail. The present work focused on the antioxidant assay and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace.

RESULTS

Six fractions of apple polyphenols, API to APVI, were acquired through extraction and purification using absorbent macroporous resin. Fraction APIII, eluted by 40% aqueous ethanol, had the highest content of total phenolics (1.48 ± 0.03 g gallic acid equivalents g(-1) dry apple pomace), which consisted of chlorogenic acid, caffeic acid, syrigin, procyanidin B2, (-)-epicatechin, cinnamic acid, coumaric acid and quercetin. Antioxidant assays showed that APIII had the strongest antioxidant activity of DPPH radical scavenging rate (90.96% ± 10.23%), ABTS radical inhibition rate (89.78% ± 6.54%) and the strongest reducing power (8.30 ± 0.71 µmol Trolox equivalents kg(-1) dry apple pomace). It also indicated that procyanidin B2, chlorogenic acid, (-)-epicatechin and quercetin had stronger antioxidant capacity than other phenols.

CONCLUSION

Our data suggested that extracts from industrial apple pomace were rich in phenols and exhibited potent antioxidant activity. Extraction of polyphenols from industrial apple pomace would bring a great benefit and improve development of apple juice and cider industries.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bai X
College of Food Science, Engineering, Northwest A&F University, Yangling, China.
Zhang H
No affiliation info available
Ren S
No affiliation info available

MeSH

AntioxidantsBenzothiazolesBiflavonoidsBiphenyl CompoundsCatechinChlorogenic AcidChromatography, High Pressure LiquidFood IndustryFruitMalusPicratesPlant ExtractsPolyphenolsProanthocyanidinsQuercetinSulfonic AcidsWaste Products

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23460126

Citation

Bai, Xuelian, et al. "Antioxidant Activity and HPLC Analysis of Polyphenol-enriched Extracts From Industrial Apple Pomace." Journal of the Science of Food and Agriculture, vol. 93, no. 10, 2013, pp. 2502-6.
Bai X, Zhang H, Ren S. Antioxidant activity and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace. J Sci Food Agric. 2013;93(10):2502-6.
Bai, X., Zhang, H., & Ren, S. (2013). Antioxidant activity and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace. Journal of the Science of Food and Agriculture, 93(10), 2502-6. https://doi.org/10.1002/jsfa.6066
Bai X, Zhang H, Ren S. Antioxidant Activity and HPLC Analysis of Polyphenol-enriched Extracts From Industrial Apple Pomace. J Sci Food Agric. 2013 Aug 15;93(10):2502-6. PubMed PMID: 23460126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace. AU - Bai,Xuelian, AU - Zhang,Huawei, AU - Ren,Shuang, Y1 - 2013/03/04/ PY - 2012/09/15/received PY - 2012/12/17/revised PY - 2013/01/25/accepted PY - 2013/3/6/entrez PY - 2013/3/6/pubmed PY - 2014/1/15/medline SP - 2502 EP - 6 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 93 IS - 10 N2 - BACKGROUND: Phenolic compounds are the predominant ingredients in apple pomace. However, polyphenols from industrial apple pomace, which usually consists of several cultivars, have not been studied in detail. The present work focused on the antioxidant assay and HPLC analysis of polyphenol-enriched extracts from industrial apple pomace. RESULTS: Six fractions of apple polyphenols, API to APVI, were acquired through extraction and purification using absorbent macroporous resin. Fraction APIII, eluted by 40% aqueous ethanol, had the highest content of total phenolics (1.48 ± 0.03 g gallic acid equivalents g(-1) dry apple pomace), which consisted of chlorogenic acid, caffeic acid, syrigin, procyanidin B2, (-)-epicatechin, cinnamic acid, coumaric acid and quercetin. Antioxidant assays showed that APIII had the strongest antioxidant activity of DPPH radical scavenging rate (90.96% ± 10.23%), ABTS radical inhibition rate (89.78% ± 6.54%) and the strongest reducing power (8.30 ± 0.71 µmol Trolox equivalents kg(-1) dry apple pomace). It also indicated that procyanidin B2, chlorogenic acid, (-)-epicatechin and quercetin had stronger antioxidant capacity than other phenols. CONCLUSION: Our data suggested that extracts from industrial apple pomace were rich in phenols and exhibited potent antioxidant activity. Extraction of polyphenols from industrial apple pomace would bring a great benefit and improve development of apple juice and cider industries. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/23460126/Antioxidant_activity_and_HPLC_analysis_of_polyphenol_enriched_extracts_from_industrial_apple_pomace_ L2 - https://doi.org/10.1002/jsfa.6066 DB - PRIME DP - Unbound Medicine ER -