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Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury.
Osteoarthritis Cartilage. 2013 May; 21(5):746-55.OC

Abstract

OBJECTIVE

Develop a non-terminal animal model of acute joint injury that demonstrates clinical and morphological evidence of early post-traumatic osteoarthritis (PTOA).

METHODS

An osteochondral (OC) fragment was created arthroscopically in one metacarpophalangeal (MCP) joint of 11 horses and the contralateral joint was sham operated. Eleven additional horses served as unoperated controls. Every 2 weeks, force plate analysis, flexion response, joint circumference, and synovial effusion scores were recorded. At weeks 0 and 16, radiographs (all horses) and arthroscopic videos (OC injured and sham joints) were graded. At week 16, synovium and cartilage biopsies were taken arthroscopically from OC injured and sham joints for histologic evaluation and the OC fragment was removed.

RESULTS

OC fragments were successfully created and horses were free of clinical lameness after fragment removal. Forelimb gait asymmetry was observed at week 2 (P = 0.0012), while joint circumference (P < 0.0001) and effusion scores (P < 0.0001) were increased in injured limbs compared to baseline from weeks 2 to 16. Positive flexion response of injured limbs was noted at multiple time points. Capsular enthesophytes were seen radiographically in injured limbs. Articular cartilage damage was demonstrated arthroscopically as mild wear-lines and histologically as superficial zone chondrocyte death accompanied by mild proliferation. Synovial hyperemia and fibrosis were present at the site of OC injury.

CONCLUSION

Acute OC injury to the MCP joint resulted in clinical, imaging, and histologic changes in cartilage and synovium characteristic of early PTOA. This model will be useful for defining biomarkers of early osteoarthritis and for monitoring response to therapy and surgery.

Authors+Show Affiliations

Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23467035

Citation

Boyce, M K., et al. "Non-terminal Animal Model of Post-traumatic Osteoarthritis Induced By Acute Joint Injury." Osteoarthritis and Cartilage, vol. 21, no. 5, 2013, pp. 746-55.
Boyce MK, Trumble TN, Carlson CS, et al. Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury. Osteoarthr Cartil. 2013;21(5):746-55.
Boyce, M. K., Trumble, T. N., Carlson, C. S., Groschen, D. M., Merritt, K. A., & Brown, M. P. (2013). Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury. Osteoarthritis and Cartilage, 21(5), 746-55. https://doi.org/10.1016/j.joca.2013.02.653
Boyce MK, et al. Non-terminal Animal Model of Post-traumatic Osteoarthritis Induced By Acute Joint Injury. Osteoarthr Cartil. 2013;21(5):746-55. PubMed PMID: 23467035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury. AU - Boyce,M K, AU - Trumble,T N, AU - Carlson,C S, AU - Groschen,D M, AU - Merritt,K A, AU - Brown,M P, Y1 - 2013/03/01/ PY - 2012/05/14/received PY - 2013/02/12/revised PY - 2013/02/21/accepted PY - 2013/3/8/entrez PY - 2013/3/8/pubmed PY - 2013/10/18/medline SP - 746 EP - 55 JF - Osteoarthritis and cartilage JO - Osteoarthr. Cartil. VL - 21 IS - 5 N2 - OBJECTIVE: Develop a non-terminal animal model of acute joint injury that demonstrates clinical and morphological evidence of early post-traumatic osteoarthritis (PTOA). METHODS: An osteochondral (OC) fragment was created arthroscopically in one metacarpophalangeal (MCP) joint of 11 horses and the contralateral joint was sham operated. Eleven additional horses served as unoperated controls. Every 2 weeks, force plate analysis, flexion response, joint circumference, and synovial effusion scores were recorded. At weeks 0 and 16, radiographs (all horses) and arthroscopic videos (OC injured and sham joints) were graded. At week 16, synovium and cartilage biopsies were taken arthroscopically from OC injured and sham joints for histologic evaluation and the OC fragment was removed. RESULTS: OC fragments were successfully created and horses were free of clinical lameness after fragment removal. Forelimb gait asymmetry was observed at week 2 (P = 0.0012), while joint circumference (P < 0.0001) and effusion scores (P < 0.0001) were increased in injured limbs compared to baseline from weeks 2 to 16. Positive flexion response of injured limbs was noted at multiple time points. Capsular enthesophytes were seen radiographically in injured limbs. Articular cartilage damage was demonstrated arthroscopically as mild wear-lines and histologically as superficial zone chondrocyte death accompanied by mild proliferation. Synovial hyperemia and fibrosis were present at the site of OC injury. CONCLUSION: Acute OC injury to the MCP joint resulted in clinical, imaging, and histologic changes in cartilage and synovium characteristic of early PTOA. This model will be useful for defining biomarkers of early osteoarthritis and for monitoring response to therapy and surgery. SN - 1522-9653 UR - https://www.unboundmedicine.com/medline/citation/23467035/Non_terminal_animal_model_of_post_traumatic_osteoarthritis_induced_by_acute_joint_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1063-4584(13)00703-6 DB - PRIME DP - Unbound Medicine ER -