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Efficacy of single dose of a bivalent vaccine containing inactivated Newcastle disease virus and reassortant highly pathogenic avian influenza H5N1 virus against lethal HPAI and NDV infection in chickens.
PLoS One. 2013; 8(3):e58186.Plos

Abstract

Highly pathogenic avian influenza (HPAI) and Newcastle disease (ND) are 2 devastating diseases of poultry, which cause great economic losses to the poultry industry. In the present study, we developed a bivalent vaccine containing antigens of inactivated ND and reassortant HPAI H5N1 viruses as a candidate poultry vaccine, and we evaluated its immunogenicity and protective efficacy in specific pathogen-free chickens. The 6:2 reassortant H5N1 vaccine strain containing the surface genes of the A/Chicken/Korea/ES/2003(H5N1) virus was successfully generated by reverse genetics. A polybasic cleavage site of the hemagglutinin segment was replaced by a monobasic cleavage site. We characterized the reverse genetics-derived reassortant HPAI H5N1 clade 2.5 vaccine strain by evaluating its growth kinetics in eggs, minimum effective dose in chickens, and cross-clade immunogenicity against HPAI clade 1 and 2. The bivalent vaccine was prepared by emulsifying inactivated ND (La Sota strain) and reassortant HPAI viruses with Montanide ISA 70 adjuvant. A single immunization with this vaccine induced high levels of hemagglutination-inhibiting antibody titers and protected chickens against a lethal challenge with the wild-type HPAI and ND viruses. Our results demonstrate that the bivalent, inactivated vaccine developed in this study is a promising approach for the control of both HPAI H5N1 and ND viral infections.

Authors+Show Affiliations

Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23469269

Citation

Lee, Dong-Hun, et al. "Efficacy of Single Dose of a Bivalent Vaccine Containing Inactivated Newcastle Disease Virus and Reassortant Highly Pathogenic Avian Influenza H5N1 Virus Against Lethal HPAI and NDV Infection in Chickens." PloS One, vol. 8, no. 3, 2013, pp. e58186.
Lee DH, Park JK, Kwon JH, et al. Efficacy of single dose of a bivalent vaccine containing inactivated Newcastle disease virus and reassortant highly pathogenic avian influenza H5N1 virus against lethal HPAI and NDV infection in chickens. PLoS One. 2013;8(3):e58186.
Lee, D. H., Park, J. K., Kwon, J. H., Yuk, S. S., Erdene-Ochir, T. O., Jang, Y. H., Seong, B. L., Lee, J. B., Park, S. Y., Choi, I. S., & Song, C. S. (2013). Efficacy of single dose of a bivalent vaccine containing inactivated Newcastle disease virus and reassortant highly pathogenic avian influenza H5N1 virus against lethal HPAI and NDV infection in chickens. PloS One, 8(3), e58186. https://doi.org/10.1371/journal.pone.0058186
Lee DH, et al. Efficacy of Single Dose of a Bivalent Vaccine Containing Inactivated Newcastle Disease Virus and Reassortant Highly Pathogenic Avian Influenza H5N1 Virus Against Lethal HPAI and NDV Infection in Chickens. PLoS One. 2013;8(3):e58186. PubMed PMID: 23469269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of single dose of a bivalent vaccine containing inactivated Newcastle disease virus and reassortant highly pathogenic avian influenza H5N1 virus against lethal HPAI and NDV infection in chickens. AU - Lee,Dong-Hun, AU - Park,Jae-Keun, AU - Kwon,Jung-Hoon, AU - Yuk,Seong-Su, AU - Erdene-Ochir,Tseren-Ochir, AU - Jang,Yo-Han, AU - Seong,Baik-Lin, AU - Lee,Joong-Bok, AU - Park,Seung-Yong, AU - Choi,In-Soo, AU - Song,Chang-Seon, Y1 - 2013/03/01/ PY - 2012/12/06/received PY - 2013/01/31/accepted PY - 2013/3/8/entrez PY - 2013/3/8/pubmed PY - 2013/8/28/medline SP - e58186 EP - e58186 JF - PloS one JO - PLoS One VL - 8 IS - 3 N2 - Highly pathogenic avian influenza (HPAI) and Newcastle disease (ND) are 2 devastating diseases of poultry, which cause great economic losses to the poultry industry. In the present study, we developed a bivalent vaccine containing antigens of inactivated ND and reassortant HPAI H5N1 viruses as a candidate poultry vaccine, and we evaluated its immunogenicity and protective efficacy in specific pathogen-free chickens. The 6:2 reassortant H5N1 vaccine strain containing the surface genes of the A/Chicken/Korea/ES/2003(H5N1) virus was successfully generated by reverse genetics. A polybasic cleavage site of the hemagglutinin segment was replaced by a monobasic cleavage site. We characterized the reverse genetics-derived reassortant HPAI H5N1 clade 2.5 vaccine strain by evaluating its growth kinetics in eggs, minimum effective dose in chickens, and cross-clade immunogenicity against HPAI clade 1 and 2. The bivalent vaccine was prepared by emulsifying inactivated ND (La Sota strain) and reassortant HPAI viruses with Montanide ISA 70 adjuvant. A single immunization with this vaccine induced high levels of hemagglutination-inhibiting antibody titers and protected chickens against a lethal challenge with the wild-type HPAI and ND viruses. Our results demonstrate that the bivalent, inactivated vaccine developed in this study is a promising approach for the control of both HPAI H5N1 and ND viral infections. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23469269/Efficacy_of_single_dose_of_a_bivalent_vaccine_containing_inactivated_Newcastle_disease_virus_and_reassortant_highly_pathogenic_avian_influenza_H5N1_virus_against_lethal_HPAI_and_NDV_infection_in_chickens_ L2 - https://dx.plos.org/10.1371/journal.pone.0058186 DB - PRIME DP - Unbound Medicine ER -