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N-stearoyl-L-tyrosine ameliorates sevoflurane induced neuroapoptosis via MEK/ERK1/2 MAPK signaling pathway in the developing brain.
Neurosci Lett. 2013 Apr 29; 541:167-72.NL

Abstract

N-arachidonoylethanolamine (AEA) plays a crucial neuroprotective role in certain neurodegenerative diseases. Our recent studies suggested that AEA analog N-stearoyl-l-tyrosine (NsTyr) could protect neurons from apoptosis and improve hippocampus-dependent learning and memory deficits. The present study was designed to determine the neuroprotective effect of NsTyr on developmental sevoflurane neurotoxicity using primary hippocampal neuronal cultures and rat pups. We found that NsTyr could decrease cell viability and reduce apoptosis in sevoflurane treated neuronal cultures. In addition, NsTyr attenuated sevoflurane-induced apoptosis by modulating Caspase-3 and Bcl-2 in vivo. Moreover, sevoflurane exposure led to an inhibition of phospho-ERK1/2, which was rescued by NsTyr. Administration of U0126 (an inhibitor of MEK) abolished the neuroprotective effect of NsTyr on sevoflurane neurotoxicity both in vitro and in vivo. Finally, administration of NsTyr improved the learning and memory disorders induced by postnatal sevoflurane exposure without alteration in locomotor activity. These results indicated that AEA analog NsTyr protects developing brain against developmental sevoflurane neurotoxicity possibly through MEK/ERK1/2 MAPK signaling pathway.

Authors+Show Affiliations

Department of Anesthesiology, Zhejiang Provincial People's Hospital, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23470632

Citation

Wang, Wen-Yuan, et al. "N-stearoyl-L-tyrosine Ameliorates Sevoflurane Induced Neuroapoptosis Via MEK/ERK1/2 MAPK Signaling Pathway in the Developing Brain." Neuroscience Letters, vol. 541, 2013, pp. 167-72.
Wang WY, Yang R, Hu SF, et al. N-stearoyl-L-tyrosine ameliorates sevoflurane induced neuroapoptosis via MEK/ERK1/2 MAPK signaling pathway in the developing brain. Neurosci Lett. 2013;541:167-72.
Wang, W. Y., Yang, R., Hu, S. F., Wang, H., Ma, Z. W., & Lu, Y. (2013). N-stearoyl-L-tyrosine ameliorates sevoflurane induced neuroapoptosis via MEK/ERK1/2 MAPK signaling pathway in the developing brain. Neuroscience Letters, 541, 167-72. https://doi.org/10.1016/j.neulet.2013.02.041
Wang WY, et al. N-stearoyl-L-tyrosine Ameliorates Sevoflurane Induced Neuroapoptosis Via MEK/ERK1/2 MAPK Signaling Pathway in the Developing Brain. Neurosci Lett. 2013 Apr 29;541:167-72. PubMed PMID: 23470632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-stearoyl-L-tyrosine ameliorates sevoflurane induced neuroapoptosis via MEK/ERK1/2 MAPK signaling pathway in the developing brain. AU - Wang,Wen-Yuan, AU - Yang,Rui, AU - Hu,Shuang-Fei, AU - Wang,Hua, AU - Ma,Zheng-Wen, AU - Lu,Yang, Y1 - 2013/03/05/ PY - 2012/11/28/received PY - 2013/02/12/revised PY - 2013/02/25/accepted PY - 2013/3/9/entrez PY - 2013/3/9/pubmed PY - 2013/9/4/medline SP - 167 EP - 72 JF - Neuroscience letters JO - Neurosci. Lett. VL - 541 N2 - N-arachidonoylethanolamine (AEA) plays a crucial neuroprotective role in certain neurodegenerative diseases. Our recent studies suggested that AEA analog N-stearoyl-l-tyrosine (NsTyr) could protect neurons from apoptosis and improve hippocampus-dependent learning and memory deficits. The present study was designed to determine the neuroprotective effect of NsTyr on developmental sevoflurane neurotoxicity using primary hippocampal neuronal cultures and rat pups. We found that NsTyr could decrease cell viability and reduce apoptosis in sevoflurane treated neuronal cultures. In addition, NsTyr attenuated sevoflurane-induced apoptosis by modulating Caspase-3 and Bcl-2 in vivo. Moreover, sevoflurane exposure led to an inhibition of phospho-ERK1/2, which was rescued by NsTyr. Administration of U0126 (an inhibitor of MEK) abolished the neuroprotective effect of NsTyr on sevoflurane neurotoxicity both in vitro and in vivo. Finally, administration of NsTyr improved the learning and memory disorders induced by postnatal sevoflurane exposure without alteration in locomotor activity. These results indicated that AEA analog NsTyr protects developing brain against developmental sevoflurane neurotoxicity possibly through MEK/ERK1/2 MAPK signaling pathway. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/23470632/N_stearoyl_L_tyrosine_ameliorates_sevoflurane_induced_neuroapoptosis_via_MEK/ERK1/2_MAPK_signaling_pathway_in_the_developing_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(13)00177-8 DB - PRIME DP - Unbound Medicine ER -