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The use of near infrared spectroscopy and multivariate techniques to differentiate Escherichia coli and Salmonella Enteritidis inoculated into pulp juice.
J Microbiol Methods. 2013 May; 93(2):90-4.JM

Abstract

This study shows the application and usefulness of near infrared (NIR) transflectance spectra measurements in the identification and classification of Escherichia coli and Salmonella Enteritidis from commercial fruit pulp (pineapple). Principal component analysis (PCA), soft independent modeling of class analogy (SIMCA) analysis and partial least-squares discriminant analysis (PLS-DA) were used in the analysis. It was not possible to obtain total separation between the samples using PCA and SIMCA. PLS-DA presented good performance achieving prediction ability of 87.5% for E. coli and 88.3% for S. Enteritidis, respectively. For the best models, the sensitivity and specificity was 0.87 and 0.83 for PLS-DA with second derivative spectra. These results suggest that NIR spectroscopy and PLS-DA can be used to discriminate and detect bacteria in fruit pulp for modeling linear class boundaries.

Authors+Show Affiliations

Universidade Federal do Rio Grande do Norte, Instituto de Química, Programa de Pós-Graduação em Química, Grupo de Pesquisa em Quimiometria Aplicada, CEP 59072-970 - Natal, RN, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23470963

Citation

de Sousa Marques, Aline, et al. "The Use of Near Infrared Spectroscopy and Multivariate Techniques to Differentiate Escherichia Coli and Salmonella Enteritidis Inoculated Into Pulp Juice." Journal of Microbiological Methods, vol. 93, no. 2, 2013, pp. 90-4.
de Sousa Marques A, Nicácio JT, Cidral TA, et al. The use of near infrared spectroscopy and multivariate techniques to differentiate Escherichia coli and Salmonella Enteritidis inoculated into pulp juice. J Microbiol Methods. 2013;93(2):90-4.
de Sousa Marques, A., Nicácio, J. T., Cidral, T. A., de Melo, M. C., & de Lima, K. M. (2013). The use of near infrared spectroscopy and multivariate techniques to differentiate Escherichia coli and Salmonella Enteritidis inoculated into pulp juice. Journal of Microbiological Methods, 93(2), 90-4. https://doi.org/10.1016/j.mimet.2013.02.003
de Sousa Marques A, et al. The Use of Near Infrared Spectroscopy and Multivariate Techniques to Differentiate Escherichia Coli and Salmonella Enteritidis Inoculated Into Pulp Juice. J Microbiol Methods. 2013;93(2):90-4. PubMed PMID: 23470963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of near infrared spectroscopy and multivariate techniques to differentiate Escherichia coli and Salmonella Enteritidis inoculated into pulp juice. AU - de Sousa Marques,Aline, AU - Nicácio,Jábine Talitta Nunes, AU - Cidral,Tiago André, AU - de Melo,Maria Celeste Nunes, AU - de Lima,Kássio Michell Gomes, Y1 - 2013/03/05/ PY - 2012/12/10/received PY - 2013/02/11/revised PY - 2013/02/11/accepted PY - 2013/3/9/entrez PY - 2013/3/9/pubmed PY - 2013/10/19/medline SP - 90 EP - 4 JF - Journal of microbiological methods JO - J Microbiol Methods VL - 93 IS - 2 N2 - This study shows the application and usefulness of near infrared (NIR) transflectance spectra measurements in the identification and classification of Escherichia coli and Salmonella Enteritidis from commercial fruit pulp (pineapple). Principal component analysis (PCA), soft independent modeling of class analogy (SIMCA) analysis and partial least-squares discriminant analysis (PLS-DA) were used in the analysis. It was not possible to obtain total separation between the samples using PCA and SIMCA. PLS-DA presented good performance achieving prediction ability of 87.5% for E. coli and 88.3% for S. Enteritidis, respectively. For the best models, the sensitivity and specificity was 0.87 and 0.83 for PLS-DA with second derivative spectra. These results suggest that NIR spectroscopy and PLS-DA can be used to discriminate and detect bacteria in fruit pulp for modeling linear class boundaries. SN - 1872-8359 UR - https://www.unboundmedicine.com/medline/citation/23470963/The_use_of_near_infrared_spectroscopy_and_multivariate_techniques_to_differentiate_Escherichia_coli_and_Salmonella_Enteritidis_inoculated_into_pulp_juice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7012(13)00046-8 DB - PRIME DP - Unbound Medicine ER -