Tags

Type your tag names separated by a space and hit enter

Protective effect of FTY720 against sevoflurane-induced developmental neurotoxicity in rats.
Cell Biochem Biophys. 2013 Nov; 67(2):591-8.CB

Abstract

Sevoflurane, a common used inhaled anaesthetic, induces neuronal apoptosis in preclinical studies and correlates with functional neurological impairment. We investigated whether FTY720, a known sphingosine-1 phosphate (S1P) receptor agonist, could exert neuroprotective effect against sevoflurane-induced neurotoxicity. Neuroprotective effect of FTY720 was evaluated in vitro in hippocampal neuronal cells from neonatal rats and in vivo in rat pups. In vitro cell apoptosis was determined by flow cytometry after exposure to 3% sevoflurane for different period of time, or after 6-h exposure to sevoflurane with the presence of FTY720, SEW2871 (selective S1P1 receptor agonist) or combination of FTY720 and VPC23019 (S1P antagonist). Western blot analysis was performed with hippocampal tissue from rat pups exposed to 3% sevoflurane for 6 h with or without pre-treatment with FTY720 injection. Neurological function tests were also performed with rat pups exposed to 3% sevoflurane for 6 h with or without pre-treatment with FTY720 injection. FTY720, at nanomolar concentration, significantly prevents sevoflurane-induced neuronal apoptosis. SEW2871 showed similar neuroprotective effect to FTY720, whereas VPC23019 abrogated the neuroprotective effect of FTY720 when given together. Western blots results demonstrated that FTY710 significantly preserved the level of phosphorylated ERK1/2, Bcl-2 and Bax. Although anaesthetic treatment did not affect general health and emotional status, sevoflurane-induced cognitive impairment in rat models. Administration of FTY720 at 1 mg/kg significantly attenuated sevoflurane-induced neurocognitive impairment. Although further studies are needed to evaluate the feasibility of clinical usage of FTY720 as neuroprotective agent, the study provides preclinical experimental evidence for the efficacy of FTY720 against sevoflurane-induced developmental neurotoxicity.

Authors+Show Affiliations

Department of Anesthesiology, Provincial Hospital Affiliated to Shandong University, Shandong University, No. 324, JingWu Road, Jinan, 250021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23471662

Citation

Zhou, Hui, et al. "Protective Effect of FTY720 Against Sevoflurane-induced Developmental Neurotoxicity in Rats." Cell Biochemistry and Biophysics, vol. 67, no. 2, 2013, pp. 591-8.
Zhou H, Li S, Niu X, et al. Protective effect of FTY720 against sevoflurane-induced developmental neurotoxicity in rats. Cell Biochem Biophys. 2013;67(2):591-8.
Zhou, H., Li, S., Niu, X., Wang, P., Wang, J., & Zhang, M. (2013). Protective effect of FTY720 against sevoflurane-induced developmental neurotoxicity in rats. Cell Biochemistry and Biophysics, 67(2), 591-8. https://doi.org/10.1007/s12013-013-9546-3
Zhou H, et al. Protective Effect of FTY720 Against Sevoflurane-induced Developmental Neurotoxicity in Rats. Cell Biochem Biophys. 2013;67(2):591-8. PubMed PMID: 23471662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of FTY720 against sevoflurane-induced developmental neurotoxicity in rats. AU - Zhou,Hui, AU - Li,Song, AU - Niu,Xinhuan, AU - Wang,Ping, AU - Wang,Junnan, AU - Zhang,Mengyuan, PY - 2013/3/9/entrez PY - 2013/3/9/pubmed PY - 2014/6/8/medline SP - 591 EP - 8 JF - Cell biochemistry and biophysics JO - Cell Biochem. Biophys. VL - 67 IS - 2 N2 - Sevoflurane, a common used inhaled anaesthetic, induces neuronal apoptosis in preclinical studies and correlates with functional neurological impairment. We investigated whether FTY720, a known sphingosine-1 phosphate (S1P) receptor agonist, could exert neuroprotective effect against sevoflurane-induced neurotoxicity. Neuroprotective effect of FTY720 was evaluated in vitro in hippocampal neuronal cells from neonatal rats and in vivo in rat pups. In vitro cell apoptosis was determined by flow cytometry after exposure to 3% sevoflurane for different period of time, or after 6-h exposure to sevoflurane with the presence of FTY720, SEW2871 (selective S1P1 receptor agonist) or combination of FTY720 and VPC23019 (S1P antagonist). Western blot analysis was performed with hippocampal tissue from rat pups exposed to 3% sevoflurane for 6 h with or without pre-treatment with FTY720 injection. Neurological function tests were also performed with rat pups exposed to 3% sevoflurane for 6 h with or without pre-treatment with FTY720 injection. FTY720, at nanomolar concentration, significantly prevents sevoflurane-induced neuronal apoptosis. SEW2871 showed similar neuroprotective effect to FTY720, whereas VPC23019 abrogated the neuroprotective effect of FTY720 when given together. Western blots results demonstrated that FTY710 significantly preserved the level of phosphorylated ERK1/2, Bcl-2 and Bax. Although anaesthetic treatment did not affect general health and emotional status, sevoflurane-induced cognitive impairment in rat models. Administration of FTY720 at 1 mg/kg significantly attenuated sevoflurane-induced neurocognitive impairment. Although further studies are needed to evaluate the feasibility of clinical usage of FTY720 as neuroprotective agent, the study provides preclinical experimental evidence for the efficacy of FTY720 against sevoflurane-induced developmental neurotoxicity. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/23471662/Protective_effect_of_FTY720_against_sevoflurane_induced_developmental_neurotoxicity_in_rats_ L2 - https://dx.doi.org/10.1007/s12013-013-9546-3 DB - PRIME DP - Unbound Medicine ER -