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Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments.
PLoS One. 2013; 8(3):e58316.Plos

Abstract

In many North American rivers, populations of multiple species of non-native cyprinid fishes are present, including black carp (Mylpharyngodon piceus), grass carp (Ctenopharyngodon idella), bighead carp (Hypophthalmichthys nobilis), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio), and goldfish (Carassius auratus). All six of these species are found in the Mississippi River basin and tracking their invasion has proven difficult, particularly where abundance is low. Knowledge of the location of the invasion front is valuable to natural resource managers because future ecological and economic damages can be most effectively prevented when populations are low. To test the accuracy of environmental DNA (eDNA) as an early indicator of species occurrence and relative abundance, we applied eDNA technology to the six non-native cyprinid species putatively present in a 2.6 river mile stretch of the Chicago (IL, USA) canal system that was subsequently treated with piscicide. The proportion of water samples yielding positive detections increased with relative abundance of the six species, as indicated by the number of carcasses recovered after poisoning. New markers for black carp, grass carp, and a common carp/goldfish are reported and details of the marker testing to ensure specificity are provided.

Authors+Show Affiliations

Department of Biology, Institute for Great Lakes Research, Central Michigan University, Mount Pleasant, Michigan, USA. mahon2a@cmich.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

Language

eng

PubMed ID

23472178

Citation

Mahon, Andrew R., et al. "Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments." PloS One, vol. 8, no. 3, 2013, pp. e58316.
Mahon AR, Jerde CL, Galaska M, et al. Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments. PLoS ONE. 2013;8(3):e58316.
Mahon, A. R., Jerde, C. L., Galaska, M., Bergner, J. L., Chadderton, W. L., Lodge, D. M., Hunter, M. E., & Nico, L. G. (2013). Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments. PloS One, 8(3), e58316. https://doi.org/10.1371/journal.pone.0058316
Mahon AR, et al. Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments. PLoS ONE. 2013;8(3):e58316. PubMed PMID: 23472178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments. AU - Mahon,Andrew R, AU - Jerde,Christopher L, AU - Galaska,Matthew, AU - Bergner,Jennifer L, AU - Chadderton,W Lindsay, AU - Lodge,David M, AU - Hunter,Margaret E, AU - Nico,Leo G, Y1 - 2013/03/05/ PY - 2012/10/29/received PY - 2013/02/01/accepted PY - 2013/3/9/entrez PY - 2013/3/9/pubmed PY - 2013/12/16/medline SP - e58316 EP - e58316 JF - PloS one JO - PLoS ONE VL - 8 IS - 3 N2 - In many North American rivers, populations of multiple species of non-native cyprinid fishes are present, including black carp (Mylpharyngodon piceus), grass carp (Ctenopharyngodon idella), bighead carp (Hypophthalmichthys nobilis), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio), and goldfish (Carassius auratus). All six of these species are found in the Mississippi River basin and tracking their invasion has proven difficult, particularly where abundance is low. Knowledge of the location of the invasion front is valuable to natural resource managers because future ecological and economic damages can be most effectively prevented when populations are low. To test the accuracy of environmental DNA (eDNA) as an early indicator of species occurrence and relative abundance, we applied eDNA technology to the six non-native cyprinid species putatively present in a 2.6 river mile stretch of the Chicago (IL, USA) canal system that was subsequently treated with piscicide. The proportion of water samples yielding positive detections increased with relative abundance of the six species, as indicated by the number of carcasses recovered after poisoning. New markers for black carp, grass carp, and a common carp/goldfish are reported and details of the marker testing to ensure specificity are provided. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23472178/Validation_of_eDNA_surveillance_sensitivity_for_detection_of_Asian_carps_in_controlled_and_field_experiments_ L2 - http://dx.plos.org/10.1371/journal.pone.0058316 DB - PRIME DP - Unbound Medicine ER -