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Oropharyngeal cancers: significance of HPV16 detection in neck lymph nodes.
J Clin Virol. 2013 Jun; 57(2):120-4.JC

Abstract

BACKGROUND

An increasing proportion of oropharyngeal squamous cell carcinomas (OPSCCs) is associated with human papillomavirus (HPV) type 16 infection. Several authors have suggested that HR-HPV DNA could be used as a marker of metastases in cervical cancers. Although HPV16 DNA has been detected in neck lymph node (LN) metastases of HPV16-positive OPSCC, its significance remains controversial. Does this presence correlate to metastatic involvement or is it just the consequence of LN filter function?

OBJECTIVES

This study aims to analyse the relationship between HPV16 detection in neck LNs of HPV16-positive OPSCC and their pathological status.

STUDY DESIGN

HP16-viral load (VL) was quantified by real-time-polymerase-chain reaction in primary tumours and neck LNs, in 11 patients with HPV16-positive OPSCC and in three patients with HPV16-negative OPSCC. HPV16 in situ hybridisation and p16 immunohistochemistry were performed in all LNs.

RESULTS

A total of 45 LN levels were assessed. HPV16 DNA was not identified in HPV16-negative OPSCC LNs. All metastatic LNs from HPV16-positive OPSCC had a high VL and the viral DNA was located within tumoural cells. Among 27 pathologically tumour-free LN (PTFLN) levels 16/27 had no detectable VL, whereas the VL was low or medium (<10(5)copies/million cells) in 8/27 and high (>10(5)copies/million cells) in 3/27 PTFLN. In the latter group, no metastatic cell was identified and the viral DNA was located in immune cells.

CONCLUSION

HPV16 detection in LN is explained by its presence within either metastatic cells or immune cells. HPV16 detection in PTFLN is not necessarily correlated to occult LN metastases.

Authors+Show Affiliations

Department of Head and Neck, Institut de Cancérologie Gustave Roussy, Villejuif, France. mirghani_fr@yahoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

23478163

Citation

Mirghani, Haïtham, et al. "Oropharyngeal Cancers: Significance of HPV16 Detection in Neck Lymph Nodes." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 57, no. 2, 2013, pp. 120-4.
Mirghani H, Ferchiou M, Moreau F, et al. Oropharyngeal cancers: significance of HPV16 detection in neck lymph nodes. J Clin Virol. 2013;57(2):120-4.
Mirghani, H., Ferchiou, M., Moreau, F., Vourexakis, Z., Amen, F., Breuskin, I., Lefèvre, M., Casiraghi, O., Drusch, F., Soussan, P., Vielh, P., & St Guily, J. L. (2013). Oropharyngeal cancers: significance of HPV16 detection in neck lymph nodes. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 57(2), 120-4. https://doi.org/10.1016/j.jcv.2013.02.009
Mirghani H, et al. Oropharyngeal Cancers: Significance of HPV16 Detection in Neck Lymph Nodes. J Clin Virol. 2013;57(2):120-4. PubMed PMID: 23478163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oropharyngeal cancers: significance of HPV16 detection in neck lymph nodes. AU - Mirghani,Haïtham, AU - Ferchiou,Malek, AU - Moreau,Frederique, AU - Vourexakis,Zacharias, AU - Amen,Furrat, AU - Breuskin,Ingrid, AU - Lefèvre,Marine, AU - Casiraghi,Odile, AU - Drusch,Françoise, AU - Soussan,Patrick, AU - Vielh,Philippe, AU - St Guily,Jean Lacau, Y1 - 2013/03/07/ PY - 2012/10/26/received PY - 2013/02/05/revised PY - 2013/02/11/accepted PY - 2013/3/13/entrez PY - 2013/3/13/pubmed PY - 2013/11/14/medline SP - 120 EP - 4 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 57 IS - 2 N2 - BACKGROUND: An increasing proportion of oropharyngeal squamous cell carcinomas (OPSCCs) is associated with human papillomavirus (HPV) type 16 infection. Several authors have suggested that HR-HPV DNA could be used as a marker of metastases in cervical cancers. Although HPV16 DNA has been detected in neck lymph node (LN) metastases of HPV16-positive OPSCC, its significance remains controversial. Does this presence correlate to metastatic involvement or is it just the consequence of LN filter function? OBJECTIVES: This study aims to analyse the relationship between HPV16 detection in neck LNs of HPV16-positive OPSCC and their pathological status. STUDY DESIGN: HP16-viral load (VL) was quantified by real-time-polymerase-chain reaction in primary tumours and neck LNs, in 11 patients with HPV16-positive OPSCC and in three patients with HPV16-negative OPSCC. HPV16 in situ hybridisation and p16 immunohistochemistry were performed in all LNs. RESULTS: A total of 45 LN levels were assessed. HPV16 DNA was not identified in HPV16-negative OPSCC LNs. All metastatic LNs from HPV16-positive OPSCC had a high VL and the viral DNA was located within tumoural cells. Among 27 pathologically tumour-free LN (PTFLN) levels 16/27 had no detectable VL, whereas the VL was low or medium (<10(5)copies/million cells) in 8/27 and high (>10(5)copies/million cells) in 3/27 PTFLN. In the latter group, no metastatic cell was identified and the viral DNA was located in immune cells. CONCLUSION: HPV16 detection in LN is explained by its presence within either metastatic cells or immune cells. HPV16 detection in PTFLN is not necessarily correlated to occult LN metastases. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/23478163/Oropharyngeal_cancers:_significance_of_HPV16_detection_in_neck_lymph_nodes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(13)00050-4 DB - PRIME DP - Unbound Medicine ER -