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2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury.
Int Braz J Urol. 2013 Jan-Feb; 39(1):128-36.IB

Abstract

PURPOSE

Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate injured renal epithelial cells accompanied by a 2D map of these proteins.

MATERIALS AND METHODS

Proteins were isolated from the matrix of kidney stones containing CaOx as the major constituent using EGTA as a demineralizing agent. The effect of more than 3kDa proteins from matrix of human renal (calcium oxalate) CaOx stones was investigated on oxalate induced cell injury of MDCK renal tubular epithelial cells. A 2D map of >3kDa proteins was also generated followed by protein identification using MALDI-TOF MS.

RESULTS

The >3kDa proteins enhanced the injury caused by oxalate on MDCK cells. Also, the 2D map of proteins having MW more than 3kDa suggested the abundance of proteins in the matrix of renal stone.

CONCLUSION

Studies indicate that the mixture of >3kDa proteins in the matrix of human renal stones acts as promoter of calcium oxalate crystal nucleation and growth as it augments the renal epithelial cell injury induced by oxalate. The effect of promoters masks the inhibitors in the protein mixture thereby leading to enhanced renal cell injury. 2D map throws light on the nature of proteins present in the kidney stones.

Authors+Show Affiliations

Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat- 173234, Solan HP, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23489505

Citation

Aggarwal, K P., et al. "2D Map of Proteins From Human Renal Stone Matrix and Evaluation of Their Effect On Oxalate Induced Renal Tubular Epithelial Cell Injury." International Braz J Urol : Official Journal of the Brazilian Society of Urology, vol. 39, no. 1, 2013, pp. 128-36.
Aggarwal KP, Tandon S, Singh SK, et al. 2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury. Int Braz J Urol. 2013;39(1):128-36.
Aggarwal, K. P., Tandon, S., Singh, S. K., & Tandon, C. (2013). 2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury. International Braz J Urol : Official Journal of the Brazilian Society of Urology, 39(1), 128-36. https://doi.org/10.1590/S1677-5538.IBJU.2013.01.16
Aggarwal KP, et al. 2D Map of Proteins From Human Renal Stone Matrix and Evaluation of Their Effect On Oxalate Induced Renal Tubular Epithelial Cell Injury. Int Braz J Urol. 2013 Jan-Feb;39(1):128-36. PubMed PMID: 23489505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury. AU - Aggarwal,K P, AU - Tandon,S, AU - Singh,S K, AU - Tandon,C, PY - 2012/08/14/received PY - 2012/11/30/accepted PY - 2013/3/16/entrez PY - 2013/3/16/pubmed PY - 2013/12/16/medline SP - 128 EP - 36 JF - International braz j urol : official journal of the Brazilian Society of Urology JO - Int Braz J Urol VL - 39 IS - 1 N2 - PURPOSE: Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate injured renal epithelial cells accompanied by a 2D map of these proteins. MATERIALS AND METHODS: Proteins were isolated from the matrix of kidney stones containing CaOx as the major constituent using EGTA as a demineralizing agent. The effect of more than 3kDa proteins from matrix of human renal (calcium oxalate) CaOx stones was investigated on oxalate induced cell injury of MDCK renal tubular epithelial cells. A 2D map of >3kDa proteins was also generated followed by protein identification using MALDI-TOF MS. RESULTS: The >3kDa proteins enhanced the injury caused by oxalate on MDCK cells. Also, the 2D map of proteins having MW more than 3kDa suggested the abundance of proteins in the matrix of renal stone. CONCLUSION: Studies indicate that the mixture of >3kDa proteins in the matrix of human renal stones acts as promoter of calcium oxalate crystal nucleation and growth as it augments the renal epithelial cell injury induced by oxalate. The effect of promoters masks the inhibitors in the protein mixture thereby leading to enhanced renal cell injury. 2D map throws light on the nature of proteins present in the kidney stones. SN - 1677-6119 UR - https://www.unboundmedicine.com/medline/citation/23489505/2D_map_of_proteins_from_human_renal_stone_matrix_and_evaluation_of_their_effect_on_oxalate_induced_renal_tubular_epithelial_cell_injury_ L2 - http://brazjurol.com.br/january_february_2013//Aggarwal_128_136.htm DB - PRIME DP - Unbound Medicine ER -