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Coenzyme Q10 ameliorates the reduction in GLUT4 transporter expression induced by simvastatin in 3T3-L1 adipocytes.
Metab Syndr Relat Disord. 2013 Aug; 11(4):251-5.MS

Abstract

BACKGROUND

Statins significantly reduce cardiovascular events in a broad population of patients with hyperlipidemia. However, a small, but significant risk of new-onset diabetes has been reported in patients treated with statins. The mechanism by which statins cause diabetes has not been elucidated and therefore preventive strategies have yet to be defined.

METHOD

Our goal was to study the differing effects of a lipophilic (simvastatin) statin, hydrophilic (pravastatin) statin, and ezetimibe on glucose transporter-4 (GLUT4) protein expression in 3T3-L1 adipocytes. We hypothesized that the reductions in GLUT4 protein secondary to statin treatment would be prevented when cells were co-incubated with coenzyme Q10 (CoQ10). GLUT4 protein expression was determined using the In-Cell Western technique. Confluent adipocytes were differentiated using a hormonal cocktail for 3 days; followed by treatment with simvastatin, pravastatin, ezetimibe and CoQ10. Cell morphology was observed after treatment using phase-contrast microscopy.

RESULTS

Treatment with simvastatin (P<0.001) and simvastatin plus ezetimibe (P<0.001) significantly decreased GLUT4 protein expression in the adipocytes compared to control conditions. GLUT4 protein levels were similar to control after treatment with ezetimibe alone (P=0.52) or pravastatin (P=0.32). There was no significant difference (P=0.098) in GLUT4 protein levels after co-treatment with CoQ10 between any of the treatments and control conditions.

CONCLUSION

Our studies have shown that lipophilic statins (simvastatin) reduce the GLUT4 protein levels in adipocytes, whereas hydrophilic statins (pravastatin) or ezetimibe do not. Co-treatment with CoQ10 appears to prevent the reduction in GLUT4 protein levels caused by simvastatin.

Authors+Show Affiliations

Department of Pharmacy Practice, College of Pharmacy, Oregon State University/ Oregon Health & Science University, Portland, OR 97239, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23496027

Citation

Ganesan, Shobana, and Matthew K. Ito. "Coenzyme Q10 Ameliorates the Reduction in GLUT4 Transporter Expression Induced By Simvastatin in 3T3-L1 Adipocytes." Metabolic Syndrome and Related Disorders, vol. 11, no. 4, 2013, pp. 251-5.
Ganesan S, Ito MK. Coenzyme Q10 ameliorates the reduction in GLUT4 transporter expression induced by simvastatin in 3T3-L1 adipocytes. Metab Syndr Relat Disord. 2013;11(4):251-5.
Ganesan, S., & Ito, M. K. (2013). Coenzyme Q10 ameliorates the reduction in GLUT4 transporter expression induced by simvastatin in 3T3-L1 adipocytes. Metabolic Syndrome and Related Disorders, 11(4), 251-5. https://doi.org/10.1089/met.2012.0177
Ganesan S, Ito MK. Coenzyme Q10 Ameliorates the Reduction in GLUT4 Transporter Expression Induced By Simvastatin in 3T3-L1 Adipocytes. Metab Syndr Relat Disord. 2013;11(4):251-5. PubMed PMID: 23496027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coenzyme Q10 ameliorates the reduction in GLUT4 transporter expression induced by simvastatin in 3T3-L1 adipocytes. AU - Ganesan,Shobana, AU - Ito,Matthew K, Y1 - 2013/03/15/ PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2014/2/26/medline SP - 251 EP - 5 JF - Metabolic syndrome and related disorders JO - Metab Syndr Relat Disord VL - 11 IS - 4 N2 - BACKGROUND: Statins significantly reduce cardiovascular events in a broad population of patients with hyperlipidemia. However, a small, but significant risk of new-onset diabetes has been reported in patients treated with statins. The mechanism by which statins cause diabetes has not been elucidated and therefore preventive strategies have yet to be defined. METHOD: Our goal was to study the differing effects of a lipophilic (simvastatin) statin, hydrophilic (pravastatin) statin, and ezetimibe on glucose transporter-4 (GLUT4) protein expression in 3T3-L1 adipocytes. We hypothesized that the reductions in GLUT4 protein secondary to statin treatment would be prevented when cells were co-incubated with coenzyme Q10 (CoQ10). GLUT4 protein expression was determined using the In-Cell Western technique. Confluent adipocytes were differentiated using a hormonal cocktail for 3 days; followed by treatment with simvastatin, pravastatin, ezetimibe and CoQ10. Cell morphology was observed after treatment using phase-contrast microscopy. RESULTS: Treatment with simvastatin (P<0.001) and simvastatin plus ezetimibe (P<0.001) significantly decreased GLUT4 protein expression in the adipocytes compared to control conditions. GLUT4 protein levels were similar to control after treatment with ezetimibe alone (P=0.52) or pravastatin (P=0.32). There was no significant difference (P=0.098) in GLUT4 protein levels after co-treatment with CoQ10 between any of the treatments and control conditions. CONCLUSION: Our studies have shown that lipophilic statins (simvastatin) reduce the GLUT4 protein levels in adipocytes, whereas hydrophilic statins (pravastatin) or ezetimibe do not. Co-treatment with CoQ10 appears to prevent the reduction in GLUT4 protein levels caused by simvastatin. SN - 1557-8518 UR - https://www.unboundmedicine.com/medline/citation/23496027/Coenzyme_Q10_ameliorates_the_reduction_in_GLUT4_transporter_expression_induced_by_simvastatin_in_3T3_L1_adipocytes_ L2 - https://www.liebertpub.com/doi/10.1089/met.2012.0177?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -