Tags

Type your tag names separated by a space and hit enter

Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor.
BMC Genomics. 2013 Mar 18; 14:187.BG

Abstract

BACKGROUND

MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect - host plant interactions.

RESULTS

In this study, we systematically analyzed miRNAs from the Hessian fly. Deep-sequencing a Hessian fly larval transcriptome led to the identification of 89 miRNA species that are either identical or very similar to known miRNAs from other insects, and 184 novel miRNAs that have not been reported from other species. A genome-wide search through a draft Hessian fly genome sequence identified a total of 611 putative miRNA-encoding genes based on sequence similarity and the existence of a stem-loop structure for miRNA precursors. Analysis of the 611 putative genes revealed a striking feature: the dramatic expansion of several miRNA gene families. The largest family contained 91 genes that encoded 20 different miRNAs. Microarray analyses revealed the expression of miRNA genes was strictly regulated during Hessian fly larval development and abundance of many miRNA genes were affected by host genotypes.

CONCLUSION

The identification of a large number of miRNAs for the first time from a gall midge provides a foundation for further studies of miRNA functions in gall midge biology and behavior. The dramatic expansion of identical or similar miRNAs provides a unique system to study functional relations among miRNA iso-genes as well as changes in sequence specificity due to small changes in miRNAs and in their mRNA targets. These results may also facilitate the identification of miRNA genes for potential pest control through transgenic approaches.

Authors+Show Affiliations

Department of Entomology, Kansas State University, Manhattan, Kansas 66056, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23496979

Citation

Khajuria, Chitvan, et al. "Deep Sequencing and Genome-wide Analysis Reveals the Expansion of MicroRNA Genes in the Gall Midge Mayetiola Destructor." BMC Genomics, vol. 14, 2013, p. 187.
Khajuria C, Williams CE, El Bouhssini M, et al. Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor. BMC Genomics. 2013;14:187.
Khajuria, C., Williams, C. E., El Bouhssini, M., Whitworth, R. J., Richards, S., Stuart, J. J., & Chen, M. S. (2013). Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor. BMC Genomics, 14, 187. https://doi.org/10.1186/1471-2164-14-187
Khajuria C, et al. Deep Sequencing and Genome-wide Analysis Reveals the Expansion of MicroRNA Genes in the Gall Midge Mayetiola Destructor. BMC Genomics. 2013 Mar 18;14:187. PubMed PMID: 23496979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor. AU - Khajuria,Chitvan, AU - Williams,Christie E, AU - El Bouhssini,Mustapha, AU - Whitworth,R Jeff, AU - Richards,Stephen, AU - Stuart,Jeffrey J, AU - Chen,Ming-Shun, Y1 - 2013/03/18/ PY - 2012/09/27/received PY - 2013/03/11/accepted PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2013/9/7/medline SP - 187 EP - 187 JF - BMC genomics JO - BMC Genomics VL - 14 N2 - BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect - host plant interactions. RESULTS: In this study, we systematically analyzed miRNAs from the Hessian fly. Deep-sequencing a Hessian fly larval transcriptome led to the identification of 89 miRNA species that are either identical or very similar to known miRNAs from other insects, and 184 novel miRNAs that have not been reported from other species. A genome-wide search through a draft Hessian fly genome sequence identified a total of 611 putative miRNA-encoding genes based on sequence similarity and the existence of a stem-loop structure for miRNA precursors. Analysis of the 611 putative genes revealed a striking feature: the dramatic expansion of several miRNA gene families. The largest family contained 91 genes that encoded 20 different miRNAs. Microarray analyses revealed the expression of miRNA genes was strictly regulated during Hessian fly larval development and abundance of many miRNA genes were affected by host genotypes. CONCLUSION: The identification of a large number of miRNAs for the first time from a gall midge provides a foundation for further studies of miRNA functions in gall midge biology and behavior. The dramatic expansion of identical or similar miRNAs provides a unique system to study functional relations among miRNA iso-genes as well as changes in sequence specificity due to small changes in miRNAs and in their mRNA targets. These results may also facilitate the identification of miRNA genes for potential pest control through transgenic approaches. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/23496979/Deep_sequencing_and_genome_wide_analysis_reveals_the_expansion_of_MicroRNA_genes_in_the_gall_midge_Mayetiola_destructor_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-187 DB - PRIME DP - Unbound Medicine ER -