Tags

Type your tag names separated by a space and hit enter

H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases.
FEBS J. 2013 Jul; 280(13):3058-68.FJ

Abstract

A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. H₂-driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H₂ as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H₂-driven cofactor-recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O₂-tolerant NAD⁺-reducing hydrogenase from Ralstonia eutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications.

Authors+Show Affiliations

Department of Chemistry, University of Oxford, Oxford, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

23497170

Citation

Lauterbach, Lars, et al. "H₂-driven Cofactor Regeneration With NAD(P)⁺-reducing Hydrogenases." The FEBS Journal, vol. 280, no. 13, 2013, pp. 3058-68.
Lauterbach L, Lenz O, Vincent KA. H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases. FEBS J. 2013;280(13):3058-68.
Lauterbach, L., Lenz, O., & Vincent, K. A. (2013). H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases. The FEBS Journal, 280(13), 3058-68. https://doi.org/10.1111/febs.12245
Lauterbach L, Lenz O, Vincent KA. H₂-driven Cofactor Regeneration With NAD(P)⁺-reducing Hydrogenases. FEBS J. 2013;280(13):3058-68. PubMed PMID: 23497170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases. AU - Lauterbach,Lars, AU - Lenz,Oliver, AU - Vincent,Kylie A, Y1 - 2013/04/17/ PY - 2013/01/14/received PY - 2013/03/05/revised PY - 2013/03/08/accepted PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2013/8/31/medline SP - 3058 EP - 68 JF - The FEBS journal JO - FEBS J. VL - 280 IS - 13 N2 - A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. H₂-driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H₂ as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H₂-driven cofactor-recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O₂-tolerant NAD⁺-reducing hydrogenase from Ralstonia eutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications. SN - 1742-4658 UR - https://www.unboundmedicine.com/medline/citation/23497170/H₂_driven_cofactor_regeneration_with_NAD_P_⁺_reducing_hydrogenases_ L2 - https://doi.org/10.1111/febs.12245 DB - PRIME DP - Unbound Medicine ER -