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Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy.
Biosens Bioelectron. 2013 Jul 15; 45:174-80.BB

Abstract

The presence of enterohemorrhagic Escherichia coli bacteria in food can cause serious foodborne disease outbreaks. Early detection and identification of these pathogens is extremely important for public health and safety. Here we present a highly sensitive label-free immunosensor for the detection of pathogenic E. coli O157:H7. Anti-E. coli antibodies were covalently immobilised onto gold electrodes via a self-assembled monolayer (SAM) of mercaptohexadecanoic acid and the pathogenic bacteria were detected by electrochemical impedance spectroscopy (EIS). Surface Plasmon Resonance (SPR) was used to monitor the antibody immobilisation protocol and antibody patterned surfaces were used to demonstrate the specificity of the antibody coated surfaces against the pathogenic bacteria. The immunosensor showed a very low limit of detection (2CFU/mL) and a large linear range (3 × 10-3 × 10(4)CFU/mL). Finally, the selectivity of the sensor was demonstrated and no significant adsorption of Salmonella typhimurium was observed.

Authors+Show Affiliations

Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 10-12, 08028 Barcelona, Spain. msantos@ibecbarcelona.euNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23500360

Citation

Barreiros dos Santos, M, et al. "Highly Sensitive Detection of Pathogen Escherichia Coli O157:H7 By Electrochemical Impedance Spectroscopy." Biosensors & Bioelectronics, vol. 45, 2013, pp. 174-80.
Barreiros dos Santos M, Agusil JP, Prieto-Simón B, et al. Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy. Biosens Bioelectron. 2013;45:174-80.
Barreiros dos Santos, M., Agusil, J. P., Prieto-Simón, B., Sporer, C., Teixeira, V., & Samitier, J. (2013). Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy. Biosensors & Bioelectronics, 45, 174-80. https://doi.org/10.1016/j.bios.2013.01.009
Barreiros dos Santos M, et al. Highly Sensitive Detection of Pathogen Escherichia Coli O157:H7 By Electrochemical Impedance Spectroscopy. Biosens Bioelectron. 2013 Jul 15;45:174-80. PubMed PMID: 23500360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy. AU - Barreiros dos Santos,M, AU - Agusil,J P, AU - Prieto-Simón,B, AU - Sporer,C, AU - Teixeira,V, AU - Samitier,J, Y1 - 2013/01/29/ PY - 2012/10/15/received PY - 2012/12/28/revised PY - 2013/01/07/accepted PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2013/9/24/medline SP - 174 EP - 80 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 45 N2 - The presence of enterohemorrhagic Escherichia coli bacteria in food can cause serious foodborne disease outbreaks. Early detection and identification of these pathogens is extremely important for public health and safety. Here we present a highly sensitive label-free immunosensor for the detection of pathogenic E. coli O157:H7. Anti-E. coli antibodies were covalently immobilised onto gold electrodes via a self-assembled monolayer (SAM) of mercaptohexadecanoic acid and the pathogenic bacteria were detected by electrochemical impedance spectroscopy (EIS). Surface Plasmon Resonance (SPR) was used to monitor the antibody immobilisation protocol and antibody patterned surfaces were used to demonstrate the specificity of the antibody coated surfaces against the pathogenic bacteria. The immunosensor showed a very low limit of detection (2CFU/mL) and a large linear range (3 × 10-3 × 10(4)CFU/mL). Finally, the selectivity of the sensor was demonstrated and no significant adsorption of Salmonella typhimurium was observed. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23500360/Highly_sensitive_detection_of_pathogen_Escherichia_coli_O157:H7_by_electrochemical_impedance_spectroscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00011-0 DB - PRIME DP - Unbound Medicine ER -