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In vitro mineralization of hydroxyapatite on electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous scaffolds for tissue engineering application.
Colloids Surf B Biointerfaces 2013; 107:167-73CS

Abstract

In this study, a fibrous scaffold was prepared by electrospinning triblock PCL-PEG-PCL (PCEC) copolymer. Afterwards, in vitro biomimetic mineralization was carried out through incubation of the PCEC fibrous mats in a simulated body fluid (SBF) for different time. The apatite-deposited PCEC composite scaffolds were characterized by using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM) observation and weighing. Due to the importance of biocompatibility, rat ROS 17/2.8 osteoblasts were cultured on mineralized PCEC scaffolds, and the cell proliferation was investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assays. The obtained results confirmed that the deposited apatite had the chemical composition and crystalline phase similar to those of hydroxyapatite (HA). After 21 days incubation, the mass increase of PCEC scaffold reached up to 22%. Moreover, in vitro cell culture also confirmed that osteoblasts could attach on the mineralized composite scaffolds, and the HA-deposited PCEC mats had less cytotoxicity. So, the mineralized PCEC composite scaffolds had a great potential for tissue engineering application.

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  • Publisher Full Text

    • Authors+Show Affiliations

    Fu S
    Department of Oncology, The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, PR China. shaozhifu513@163.com
    Yang L
    No affiliation info available
    Fan J
    No affiliation info available
    Wen Q
    No affiliation info available
    Lin S
    No affiliation info available
    Wang B
    No affiliation info available
    Chen L
    No affiliation info available
    Meng X
    No affiliation info available
    Chen Y
    No affiliation info available
    Wu J
    No affiliation info available

    MeSH

    AnimalsCalciumCell AdhesionCell DeathCell ProliferationDurapatiteMineralsOsteoblastsPhosphorusPolyestersPolyethylene GlycolsRatsSpectrometry, X-Ray EmissionSpectroscopy, Fourier Transform InfraredTensile StrengthTissue EngineeringTissue ScaffoldsX-Ray Diffraction

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23500727

    Citation

    Fu, ShaoZhi, et al. "In Vitro Mineralization of Hydroxyapatite On Electrospun Poly(ε-caprolactone)-poly(ethylene Glycol)-poly(ε-caprolactone) Fibrous Scaffolds for Tissue Engineering Application." Colloids and Surfaces. B, Biointerfaces, vol. 107, 2013, pp. 167-73.
    Fu S, Yang L, Fan J, et al. In vitro mineralization of hydroxyapatite on electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous scaffolds for tissue engineering application. Colloids Surf B Biointerfaces. 2013;107:167-73.
    Fu, S., Yang, L., Fan, J., Wen, Q., Lin, S., Wang, B., ... Wu, J. (2013). In vitro mineralization of hydroxyapatite on electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous scaffolds for tissue engineering application. Colloids and Surfaces. B, Biointerfaces, 107, pp. 167-73. doi:10.1016/j.colsurfb.2013.01.068.
    Fu S, et al. In Vitro Mineralization of Hydroxyapatite On Electrospun Poly(ε-caprolactone)-poly(ethylene Glycol)-poly(ε-caprolactone) Fibrous Scaffolds for Tissue Engineering Application. Colloids Surf B Biointerfaces. 2013 Jul 1;107:167-73. PubMed PMID: 23500727.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro mineralization of hydroxyapatite on electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous scaffolds for tissue engineering application. AU - Fu,ShaoZhi, AU - Yang,LingLin, AU - Fan,Juan, AU - Wen,QingLian, AU - Lin,Sheng, AU - Wang,BiQiong, AU - Chen,LanLan, AU - Meng,XiaoHang, AU - Chen,Yue, AU - Wu,JingBo, Y1 - 2013/02/09/ PY - 2012/12/28/received PY - 2013/01/30/revised PY - 2013/01/31/accepted PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2013/10/18/medline SP - 167 EP - 73 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 107 N2 - In this study, a fibrous scaffold was prepared by electrospinning triblock PCL-PEG-PCL (PCEC) copolymer. Afterwards, in vitro biomimetic mineralization was carried out through incubation of the PCEC fibrous mats in a simulated body fluid (SBF) for different time. The apatite-deposited PCEC composite scaffolds were characterized by using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM) observation and weighing. Due to the importance of biocompatibility, rat ROS 17/2.8 osteoblasts were cultured on mineralized PCEC scaffolds, and the cell proliferation was investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assays. The obtained results confirmed that the deposited apatite had the chemical composition and crystalline phase similar to those of hydroxyapatite (HA). After 21 days incubation, the mass increase of PCEC scaffold reached up to 22%. Moreover, in vitro cell culture also confirmed that osteoblasts could attach on the mineralized composite scaffolds, and the HA-deposited PCEC mats had less cytotoxicity. So, the mineralized PCEC composite scaffolds had a great potential for tissue engineering application. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/23500727/In_vitro_mineralization_of_hydroxyapatite_on_electrospun_poly_ε_caprolactone__poly_ethylene_glycol__poly_ε_caprolactone__fibrous_scaffolds_for_tissue_engineering_application_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(13)00090-8 DB - PRIME DP - Unbound Medicine ER -