TY - JOUR T1 - Signal amplification aptamer biosensor for thrombin based on a glassy carbon electrode modified with graphene, quantum dots and gold nanoparticles. AU - Xie,Lingling, AU - You,Liqin, AU - Cao,Xiaoyu, Y1 - 2013/02/21/ PY - 2012/12/13/received PY - 2013/01/25/revised PY - 2013/02/02/accepted PY - 2013/3/19/entrez PY - 2013/3/19/pubmed PY - 2013/12/16/medline SP - 110 EP - 5 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 109 N2 - A novel electrogenerated chemiluminescence (ECL) assay for sensitive determination of thrombin is designed employing CdSe/ZnS quantum dots served as an ECL label. This ECL sensor is fabricated on graphene modified glassy carbon electrode which is then covered with a low surface coverage of gold nanoparticles (AuNPs). An aptamer is used to selectively recognize the target. The thiol-terminated aptamer is first immobilized on AuNPs/graphene modified electrode, and then thrombin is imported to form the aptamer-thrombin complexes. After blocking the nonspecifically bound oligonucleotides with MCH solution, another CdSe/ZnS quantum dots modified aptamer is hybridized with the free thiol-terminated aptamer to form a DNA complexe. A decreased ECL signal is observed upon recognition of the target thrombin. The integrated ECL intensity versus the concentration of thrombin is linear in the range from 0.01 to 50 nM. The detection limit is 10 fM. The present aptasensor also exhibits excellent selectivity, stability and reusability. This sensing system can provide a promising label-free model for aptamer-based compounds sensitive detection. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/23501724/Signal_amplification_aptamer_biosensor_for_thrombin_based_on_a_glassy_carbon_electrode_modified_with_graphene_quantum_dots_and_gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(13)00166-2 DB - PRIME DP - Unbound Medicine ER -