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Evaluation of status of arsenic, cadmium, lead and zinc levels in biological samples of normal and arthritis patients of age groups (46 - 60) and (61 - 75) years.
Clin Lab. 2013; 59(1-2):143-53.CL

Abstract

BACKGROUND

Arthritis is a chronic inflammatory disease resulting in inflammation of diarthrodial joints (particularly joints of hands, wrists, feet, knees, ankles, and shoulders), manifested by swelling and functional impairment. This study was designed to evaluate the levels of the toxic elements arsenic (As), cadmium (Cd), and lead (Pb) and correlate those with the essential trace element zinc (Zn) in biological samples (scalp hair, blood, and urine) of arthritis patients, in two age groups (46 - 60 and 61 - 75) of both genders. For comparison purposes all three biological samples were collected from gender- and age-matched non-arthritic subjects as referents.

METHODS

The As, Cd, and Pb in biological samples were analysed by electrothermal atomic absorption spectrometry, prior to microwave assisted acid digestion. The level of Zn was determined by flame atomic absorption spectrometry. The validity and accuracy of the methodology was checked by using Certified Reference Materials (CRM) from the Community Bureau of Reference (BCR) of the Commission of the European Community and with those values obtained by conventional wet acid digestion method on the same CRMs.

RESULTS

The results of this study showed that the mean values of As, Cd, and Pb were higher in blood, scalp hair, and urine samples of arthritis patients as compared to those values obtained in age-matched referent subjects. The concentration of Zn was lower in the biological samples of rheumatoid arthritis patients of both genders with respect to non-arthritic subjects. The urinary levels of the elements studied were found to be higher in the arthritis patients than in the age-matched healthy referents (p < 0.001).

CONCLUSIONS

An inverse correlation was observed between Zn and toxic elements in biological samples of arthritis patients (r = 0.612 - 0.754). Intake of certain antioxidant micronutrients, particularly a zinc supplement, may protect against the development of rheumatoid arthritis.

Authors+Show Affiliations

Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. hassanimranafridi@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

23505920

Citation

Afridi, Hassan Imran, et al. "Evaluation of Status of Arsenic, Cadmium, Lead and Zinc Levels in Biological Samples of Normal and Arthritis Patients of Age Groups (46 - 60) and (61 - 75) Years." Clinical Laboratory, vol. 59, no. 1-2, 2013, pp. 143-53.
Afridi HI, Kazi TG, Kazi N, et al. Evaluation of status of arsenic, cadmium, lead and zinc levels in biological samples of normal and arthritis patients of age groups (46 - 60) and (61 - 75) years. Clin Lab. 2013;59(1-2):143-53.
Afridi, H. I., Kazi, T. G., Kazi, N., Talpur, F. N., Shah, F., Naeemullah, ., Arain, S. S., & Brahman, K. D. (2013). Evaluation of status of arsenic, cadmium, lead and zinc levels in biological samples of normal and arthritis patients of age groups (46 - 60) and (61 - 75) years. Clinical Laboratory, 59(1-2), 143-53.
Afridi HI, et al. Evaluation of Status of Arsenic, Cadmium, Lead and Zinc Levels in Biological Samples of Normal and Arthritis Patients of Age Groups (46 - 60) and (61 - 75) Years. Clin Lab. 2013;59(1-2):143-53. PubMed PMID: 23505920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of status of arsenic, cadmium, lead and zinc levels in biological samples of normal and arthritis patients of age groups (46 - 60) and (61 - 75) years. AU - Afridi,Hassan Imran, AU - Kazi,Tasneem Gul, AU - Kazi,Naveed, AU - Talpur,Farah Naz, AU - Shah,Faheem, AU - Naeemullah,, AU - Arain,Sadaf Sadia, AU - Brahman,Kapil Dev, PY - 2013/3/20/entrez PY - 2013/3/20/pubmed PY - 2013/4/2/medline SP - 143 EP - 53 JF - Clinical laboratory JO - Clin. Lab. VL - 59 IS - 1-2 N2 - BACKGROUND: Arthritis is a chronic inflammatory disease resulting in inflammation of diarthrodial joints (particularly joints of hands, wrists, feet, knees, ankles, and shoulders), manifested by swelling and functional impairment. This study was designed to evaluate the levels of the toxic elements arsenic (As), cadmium (Cd), and lead (Pb) and correlate those with the essential trace element zinc (Zn) in biological samples (scalp hair, blood, and urine) of arthritis patients, in two age groups (46 - 60 and 61 - 75) of both genders. For comparison purposes all three biological samples were collected from gender- and age-matched non-arthritic subjects as referents. METHODS: The As, Cd, and Pb in biological samples were analysed by electrothermal atomic absorption spectrometry, prior to microwave assisted acid digestion. The level of Zn was determined by flame atomic absorption spectrometry. The validity and accuracy of the methodology was checked by using Certified Reference Materials (CRM) from the Community Bureau of Reference (BCR) of the Commission of the European Community and with those values obtained by conventional wet acid digestion method on the same CRMs. RESULTS: The results of this study showed that the mean values of As, Cd, and Pb were higher in blood, scalp hair, and urine samples of arthritis patients as compared to those values obtained in age-matched referent subjects. The concentration of Zn was lower in the biological samples of rheumatoid arthritis patients of both genders with respect to non-arthritic subjects. The urinary levels of the elements studied were found to be higher in the arthritis patients than in the age-matched healthy referents (p < 0.001). CONCLUSIONS: An inverse correlation was observed between Zn and toxic elements in biological samples of arthritis patients (r = 0.612 - 0.754). Intake of certain antioxidant micronutrients, particularly a zinc supplement, may protect against the development of rheumatoid arthritis. SN - 1433-6510 UR - https://www.unboundmedicine.com/medline/citation/23505920/Evaluation_of_status_of_arsenic_cadmium_lead_and_zinc_levels_in_biological_samples_of_normal_and_arthritis_patients_of_age_groups__46___60__and__61___75__years_ L2 - http://www.diseaseinfosearch.org/result/592 DB - PRIME DP - Unbound Medicine ER -