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Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima.
J Ethnopharmacol. 2013 May 20; 147(2):395-405.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Centipeda minima (L.) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature.

AIM OF THE STUDY

The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM).

MATERIALS AND METHODS

The following activities were investigated: antioxidant activities [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), DPPH (1,1-diphenyl-2-picrylhydrazyl)], and anti-inflammatory [lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 macrophages and paw-edema induced by λ-carrageenan (Carr)]. We also investigated the anti-inflammatory mechanism of ACM via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the edema paw. Serum NO, tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were also measured in vivo. In HPLC analysis, the fingerprint chromatogram of ACM was established.

RESULTS

ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-α, and IL-1β suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions.

CONCLUSIONS

Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.

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Authors+Show Affiliations

Huang SS
School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
Chiu CS
No affiliation info available
Lin TH
No affiliation info available
Lee MM
No affiliation info available
Lee CY
No affiliation info available
Chang SJ
No affiliation info available
Hou WC
No affiliation info available
Huang GJ
No affiliation info available
Deng JS
No affiliation info available

MeSH

AnimalsAnti-Inflammatory AgentsAntioxidantsAsteraceaeCarrageenanCatalaseCell LineCell SurvivalCyclooxygenase 2EdemaGlutathione PeroxidaseInterleukin-1betaMaleMiceMice, Inbred ICRNitric OxideNitric Oxide Synthase Type IIPhytotherapyPlant ExtractsSuperoxide DismutaseTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23506988

Citation

Huang, Shyh-Shyun, et al. "Antioxidant and Anti-inflammatory Activities of Aqueous Extract of Centipeda Minima." Journal of Ethnopharmacology, vol. 147, no. 2, 2013, pp. 395-405.
Huang SS, Chiu CS, Lin TH, et al. Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. J Ethnopharmacol. 2013;147(2):395-405.
Huang, S. S., Chiu, C. S., Lin, T. H., Lee, M. M., Lee, C. Y., Chang, S. J., Hou, W. C., Huang, G. J., & Deng, J. S. (2013). Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. Journal of Ethnopharmacology, 147(2), 395-405. https://doi.org/10.1016/j.jep.2013.03.025
Huang SS, et al. Antioxidant and Anti-inflammatory Activities of Aqueous Extract of Centipeda Minima. J Ethnopharmacol. 2013 May 20;147(2):395-405. PubMed PMID: 23506988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. AU - Huang,Shyh-Shyun, AU - Chiu,Chuan-Sung, AU - Lin,Tsung-Hui, AU - Lee,Min-Min, AU - Lee,Chao-Ying, AU - Chang,Shu-Jen, AU - Hou,Wen-Chi, AU - Huang,Guan-Jhong, AU - Deng,Jeng-Shyan, Y1 - 2013/03/15/ PY - 2012/12/13/received PY - 2013/03/06/revised PY - 2013/03/08/accepted PY - 2013/3/20/entrez PY - 2013/3/20/pubmed PY - 2013/10/24/medline SP - 395 EP - 405 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 147 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Centipeda minima (L.) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature. AIM OF THE STUDY: The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM). MATERIALS AND METHODS: The following activities were investigated: antioxidant activities [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), DPPH (1,1-diphenyl-2-picrylhydrazyl)], and anti-inflammatory [lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 macrophages and paw-edema induced by λ-carrageenan (Carr)]. We also investigated the anti-inflammatory mechanism of ACM via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the edema paw. Serum NO, tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were also measured in vivo. In HPLC analysis, the fingerprint chromatogram of ACM was established. RESULTS: ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-α, and IL-1β suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. CONCLUSIONS: Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23506988/Antioxidant_and_anti_inflammatory_activities_of_aqueous_extract_of_Centipeda_minima_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00168-2 DB - PRIME DP - Unbound Medicine ER -