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Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation.
Mol Cells. 2013 Mar; 35(3):210-8.MC

Abstract

Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to cost-effective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis.

Authors+Show Affiliations

Advanced Bio-resource Research Center, Kyungpook National University, Daegu 702-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23512334

Citation

Kim, Il-Sup, et al. "Saccharomyces Cerevisiae KNU5377 Stress Response During High-temperature Ethanol Fermentation." Molecules and Cells, vol. 35, no. 3, 2013, pp. 210-8.
Kim IS, Kim YS, Kim H, et al. Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation. Mol Cells. 2013;35(3):210-8.
Kim, I. S., Kim, Y. S., Kim, H., Jin, I., & Yoon, H. S. (2013). Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation. Molecules and Cells, 35(3), 210-8. https://doi.org/10.1007/s10059-013-2258-0
Kim IS, et al. Saccharomyces Cerevisiae KNU5377 Stress Response During High-temperature Ethanol Fermentation. Mol Cells. 2013;35(3):210-8. PubMed PMID: 23512334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation. AU - Kim,Il-Sup, AU - Kim,Young-Saeng, AU - Kim,Hyun, AU - Jin,Ingnyol, AU - Yoon,Ho-Sung, Y1 - 2013/02/18/ PY - 2012/10/08/received PY - 2012/11/08/accepted PY - 2012/11/07/revised PY - 2013/3/21/entrez PY - 2013/3/21/pubmed PY - 2013/9/21/medline SP - 210 EP - 8 JF - Molecules and cells JO - Mol Cells VL - 35 IS - 3 N2 - Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to cost-effective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis. SN - 0219-1032 UR - https://www.unboundmedicine.com/medline/citation/23512334/Saccharomyces_cerevisiae_KNU5377_stress_response_during_high_temperature_ethanol_fermentation_ L2 - http://www.molcells.org/journal/view.html?year=2013&volume=35&number=3&spage=210 DB - PRIME DP - Unbound Medicine ER -