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Role of TRPM2 cation channels in dorsal root ganglion of rats after experimental spinal cord injury.
Muscle Nerve. 2013 Dec; 48(6):945-50.MN

Abstract

INTRODUCTION

We sought to determine the contribution of oxidative stress-dependent activation of TRPM2 and L-type voltage-gated Ca(2+) channels (VGCC) in dorsal root ganglion (DRG) neurons of rats after spinal cord injury (SCI).

METHODS

The rats were divided into 4 groups: control; sham control; SCI; and SCI+nimodipine groups. The neurons of the SCI groups were also incubated with non-specific TRPM2 channel blockers, 2-aminoethoxydiphenylborate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA), before H2 O2 stimulation.

RESULTS

The [Ca(2+) ]i concentrations were higher in the SCI group than in the control groups, although their concentrations were decreased by nimodipine and 2-APB. The H2 O2 -induced TRPM2 current densities in patch-clamp experiments were decreased by ACA and 2-APB incubation. In the nimodipine group, the TRPM2 channels of neurons were not activated by H2 O2 or cumene hydroperoxide.

CONCLUSIONS

Increased Ca(2+) influx and currents in DRG neurons after spinal injury indicated TRPM2 and voltage-gated Ca(2+) channel activation.

Authors+Show Affiliations

Department of Biophysics, Medical Faculty, Suleyman Demirel University, Isparta, Turkey; Neuroscience Research Center, Suleyman Demirel University, TR-32260, Isparta, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23512594

Citation

Naziroğlu, Mustafa, et al. "Role of TRPM2 Cation Channels in Dorsal Root Ganglion of Rats After Experimental Spinal Cord Injury." Muscle & Nerve, vol. 48, no. 6, 2013, pp. 945-50.
Naziroğlu M, Uğuz AC, Ismailoğlu Ö, et al. Role of TRPM2 cation channels in dorsal root ganglion of rats after experimental spinal cord injury. Muscle Nerve. 2013;48(6):945-50.
Naziroğlu, M., Uğuz, A. C., Ismailoğlu, Ö., Çiğ, B., Özgül, C., & Borcak, M. (2013). Role of TRPM2 cation channels in dorsal root ganglion of rats after experimental spinal cord injury. Muscle & Nerve, 48(6), 945-50. https://doi.org/10.1002/mus.23844
Naziroğlu M, et al. Role of TRPM2 Cation Channels in Dorsal Root Ganglion of Rats After Experimental Spinal Cord Injury. Muscle Nerve. 2013;48(6):945-50. PubMed PMID: 23512594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of TRPM2 cation channels in dorsal root ganglion of rats after experimental spinal cord injury. AU - Naziroğlu,Mustafa, AU - Uğuz,Abdülhadi Cihangir, AU - Ismailoğlu,Özgür, AU - Çiğ,Bilal, AU - Özgül,Cemil, AU - Borcak,Muhammed, Y1 - 2013/09/11/ PY - 2013/03/11/accepted PY - 2013/3/21/entrez PY - 2013/3/21/pubmed PY - 2014/1/15/medline KW - Ca2+ signaling KW - TRPM2 cation channels KW - nimodipine KW - oxidative stress KW - peripheral pain KW - spinal injury SP - 945 EP - 50 JF - Muscle & nerve JO - Muscle Nerve VL - 48 IS - 6 N2 - INTRODUCTION: We sought to determine the contribution of oxidative stress-dependent activation of TRPM2 and L-type voltage-gated Ca(2+) channels (VGCC) in dorsal root ganglion (DRG) neurons of rats after spinal cord injury (SCI). METHODS: The rats were divided into 4 groups: control; sham control; SCI; and SCI+nimodipine groups. The neurons of the SCI groups were also incubated with non-specific TRPM2 channel blockers, 2-aminoethoxydiphenylborate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA), before H2 O2 stimulation. RESULTS: The [Ca(2+) ]i concentrations were higher in the SCI group than in the control groups, although their concentrations were decreased by nimodipine and 2-APB. The H2 O2 -induced TRPM2 current densities in patch-clamp experiments were decreased by ACA and 2-APB incubation. In the nimodipine group, the TRPM2 channels of neurons were not activated by H2 O2 or cumene hydroperoxide. CONCLUSIONS: Increased Ca(2+) influx and currents in DRG neurons after spinal injury indicated TRPM2 and voltage-gated Ca(2+) channel activation. SN - 1097-4598 UR - https://www.unboundmedicine.com/medline/citation/23512594/Role_of_TRPM2_cation_channels_in_dorsal_root_ganglion_of_rats_after_experimental_spinal_cord_injury_ L2 - https://doi.org/10.1002/mus.23844 DB - PRIME DP - Unbound Medicine ER -