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Differential fate of metabolism of a disperse dye by microorganisms Galactomyces geotrichum and Brevibacillus laterosporus and their consortium GG-BL.
J Environ Sci (China). 2012; 24(7):1295-304.JE

Abstract

The present work aims to evaluate Brown 3 REL degrading potential of developed microbial consortium GG-BL using two microbial cultures, Galactomyces geotrichum MTCC 1360 (GG) and Brevibacillus laterosporus MTCC 2298 (BL). Microbial consortium GG-BL showed 100% decolorization of a dye Brown 3 REL, while individually G. geotrichum MTCC 1360 and B. laterosporus MTCC 2298 showed 26% and 86% decolorization under aerobic condition (shaking) respectively. Measurements of biochemical oxygen demand (BOD) (76%) and chemical oxygen demand (COD) (68%) were done after decolorization by consortium GG-BL. No induction in activities of oxidoreductive enzymes found in G. geotrichum while B. laterosporus showed induction of veratryl alcohol oxidase, Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase and riboflavin reductase indicating their role in dye metabolism. Consortium GG-BL showed induction in the activities of laccase, veratryl alcohol oxidase, tyrosinase, NADH-DCIP reductase and riboflavin reductase. Two different sets of induced enzymes from G. geotrichum and B. laterosporus work together in consortium GG-BL resulting in faster degradation of dye. The degradation of Brown 3 REL was analyzed using high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FT-IR) and gas chromatography mass spectroscopy (GC-MS). Phytotoxicity study revealed that metabolites formed after degradation was significantly less toxic in nature.

Authors+Show Affiliations

Waghmode TR
Department of Biochemistry, Shivaji University, Kolhapur-416004, India. tatobawaghmode@gmail.com
Kurade MB
No affiliation info available
Kagalkar AN
No affiliation info available
Govindwar SP
No affiliation info available

MeSH

AerobiosisBiodegradation, EnvironmentalBrevibacillusChromatography, High Pressure LiquidColoring AgentsGas Chromatography-Mass SpectrometryMicrobial ConsortiaNitrogen CompoundsOrganic ChemicalsOxidation-ReductionPhaseolusSaccharomycetalesSorghumSpectroscopy, Fourier Transform InfraredToxicity TestsWaste Disposal, Fluid

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23513451

Citation

Waghmode, Tatoba R., et al. "Differential Fate of Metabolism of a Disperse Dye By Microorganisms Galactomyces Geotrichum and Brevibacillus Laterosporus and Their Consortium GG-BL." Journal of Environmental Sciences (China), vol. 24, no. 7, 2012, pp. 1295-304.
Waghmode TR, Kurade MB, Kagalkar AN, et al. Differential fate of metabolism of a disperse dye by microorganisms Galactomyces geotrichum and Brevibacillus laterosporus and their consortium GG-BL. J Environ Sci (China). 2012;24(7):1295-304.
Waghmode, T. R., Kurade, M. B., Kagalkar, A. N., & Govindwar, S. P. (2012). Differential fate of metabolism of a disperse dye by microorganisms Galactomyces geotrichum and Brevibacillus laterosporus and their consortium GG-BL. Journal of Environmental Sciences (China), 24(7), 1295-304.
Waghmode TR, et al. Differential Fate of Metabolism of a Disperse Dye By Microorganisms Galactomyces Geotrichum and Brevibacillus Laterosporus and Their Consortium GG-BL. J Environ Sci (China). 2012;24(7):1295-304. PubMed PMID: 23513451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential fate of metabolism of a disperse dye by microorganisms Galactomyces geotrichum and Brevibacillus laterosporus and their consortium GG-BL. AU - Waghmode,Tatoba R, AU - Kurade,Mayur B, AU - Kagalkar,Anuradha N, AU - Govindwar,Sanjay P, PY - 2013/3/22/entrez PY - 2012/1/1/pubmed PY - 2013/6/14/medline SP - 1295 EP - 304 JF - Journal of environmental sciences (China) JO - J Environ Sci (China) VL - 24 IS - 7 N2 - The present work aims to evaluate Brown 3 REL degrading potential of developed microbial consortium GG-BL using two microbial cultures, Galactomyces geotrichum MTCC 1360 (GG) and Brevibacillus laterosporus MTCC 2298 (BL). Microbial consortium GG-BL showed 100% decolorization of a dye Brown 3 REL, while individually G. geotrichum MTCC 1360 and B. laterosporus MTCC 2298 showed 26% and 86% decolorization under aerobic condition (shaking) respectively. Measurements of biochemical oxygen demand (BOD) (76%) and chemical oxygen demand (COD) (68%) were done after decolorization by consortium GG-BL. No induction in activities of oxidoreductive enzymes found in G. geotrichum while B. laterosporus showed induction of veratryl alcohol oxidase, Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase and riboflavin reductase indicating their role in dye metabolism. Consortium GG-BL showed induction in the activities of laccase, veratryl alcohol oxidase, tyrosinase, NADH-DCIP reductase and riboflavin reductase. Two different sets of induced enzymes from G. geotrichum and B. laterosporus work together in consortium GG-BL resulting in faster degradation of dye. The degradation of Brown 3 REL was analyzed using high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FT-IR) and gas chromatography mass spectroscopy (GC-MS). Phytotoxicity study revealed that metabolites formed after degradation was significantly less toxic in nature. SN - 1001-0742 UR - https://www.unboundmedicine.com/medline/citation/23513451/Differential_fate_of_metabolism_of_a_disperse_dye_by_microorganisms_Galactomyces_geotrichum_and_Brevibacillus_laterosporus_and_their_consortium_GG_BL_ DB - PRIME DP - Unbound Medicine ER -