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Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis.
Reprod Biol Endocrinol. 2013 Mar 21; 11:23.RB

Abstract

BACKGROUND

Whole body heat stress had detrimental effect on male reproductive function. It's known that the nuclear factor erythroid 2-related factor 2 (Nrf2) activates expression of cytoprotective genes to enable cell adaptation to protect against oxidative stress. However, it's still unclear about the exactly effects of Nrf2 on the testis. Here, we investigate the protective effect of Nrf2 on whole body heat stress-induced oxidative damage in mouse testis.

METHODS

Male mice were exposed to the elevated ambient temperature (42°C) daily for 2 h. During the period of twelve consecutive days, mice were sacrificed on days 1, 2, 4, 8 and 12 immediately following heat exposure. Testes weight, enzymatic antioxidant activities and concentrations of malondialdehyde (MDA) and glutathione (GSH) in the testes were determined and immunohistochemical detection of Nrf2 protein and mRNA expression of Nrf2-regulated genes were analyzed to assess the status of Nrf2-antioxidant system.

RESULTS

Heat-exposed mice presented significant increases in rectal, scrotal surface and body surface temperature. The concentrations of cortisol and testosterone in serum fluctuated with the number of exposed days. There were significant decrease in testes weight and relative testes weight on day 12 compared with those on other days, but significant increases in catalase (CAT) activity on day 1 and GSH level on day 4 compared with control group. The activities of total superoxide dismutase (T-SOD) and copper-zinc SOD (CuZn-SOD) increased significantly on days 8 and 12. Moreover, prominent nuclear accumulation of Nrf2 protein was observed in Leydig cells on day 2, accompanying with up-regulated mRNA levels of Nrf2-regulated genes such as Nrf2, heme oxygenase 1 (HO-1), γ-Glutamylcysteine synthetase (GCLC) and NAD (P) H: quinone oxidoreductase 1 (NQO1)) in heat-treated groups.

CONCLUSIONS

These results suggest that Nrf2 displayed nuclear accumulation and protective activity in the process of heat treated-induced oxidative stress in mouse testes, indicating that Nrf2 might be a potential target for new drugs designed to protect germ cell and Leydig cell from oxidative stress.

Authors+Show Affiliations

College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang Road, Nanjing 210095, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23514035

Citation

Li, Yansen, et al. "Protective Effects of Nuclear Factor Erythroid 2-related Factor 2 On Whole Body Heat Stress-induced Oxidative Damage in the Mouse Testis." Reproductive Biology and Endocrinology : RB&E, vol. 11, 2013, p. 23.
Li Y, Huang Y, Piao Y, et al. Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis. Reprod Biol Endocrinol. 2013;11:23.
Li, Y., Huang, Y., Piao, Y., Nagaoka, K., Watanabe, G., Taya, K., & Li, C. (2013). Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis. Reproductive Biology and Endocrinology : RB&E, 11, 23. https://doi.org/10.1186/1477-7827-11-23
Li Y, et al. Protective Effects of Nuclear Factor Erythroid 2-related Factor 2 On Whole Body Heat Stress-induced Oxidative Damage in the Mouse Testis. Reprod Biol Endocrinol. 2013 Mar 21;11:23. PubMed PMID: 23514035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis. AU - Li,Yansen, AU - Huang,Yi, AU - Piao,Yuanguo, AU - Nagaoka,Kentaro, AU - Watanabe,Gen, AU - Taya,Kazuyoshi, AU - Li,ChunMei, Y1 - 2013/03/21/ PY - 2013/02/03/received PY - 2013/03/17/accepted PY - 2013/3/22/entrez PY - 2013/3/22/pubmed PY - 2013/9/27/medline SP - 23 EP - 23 JF - Reproductive biology and endocrinology : RB&E JO - Reprod Biol Endocrinol VL - 11 N2 - BACKGROUND: Whole body heat stress had detrimental effect on male reproductive function. It's known that the nuclear factor erythroid 2-related factor 2 (Nrf2) activates expression of cytoprotective genes to enable cell adaptation to protect against oxidative stress. However, it's still unclear about the exactly effects of Nrf2 on the testis. Here, we investigate the protective effect of Nrf2 on whole body heat stress-induced oxidative damage in mouse testis. METHODS: Male mice were exposed to the elevated ambient temperature (42°C) daily for 2 h. During the period of twelve consecutive days, mice were sacrificed on days 1, 2, 4, 8 and 12 immediately following heat exposure. Testes weight, enzymatic antioxidant activities and concentrations of malondialdehyde (MDA) and glutathione (GSH) in the testes were determined and immunohistochemical detection of Nrf2 protein and mRNA expression of Nrf2-regulated genes were analyzed to assess the status of Nrf2-antioxidant system. RESULTS: Heat-exposed mice presented significant increases in rectal, scrotal surface and body surface temperature. The concentrations of cortisol and testosterone in serum fluctuated with the number of exposed days. There were significant decrease in testes weight and relative testes weight on day 12 compared with those on other days, but significant increases in catalase (CAT) activity on day 1 and GSH level on day 4 compared with control group. The activities of total superoxide dismutase (T-SOD) and copper-zinc SOD (CuZn-SOD) increased significantly on days 8 and 12. Moreover, prominent nuclear accumulation of Nrf2 protein was observed in Leydig cells on day 2, accompanying with up-regulated mRNA levels of Nrf2-regulated genes such as Nrf2, heme oxygenase 1 (HO-1), γ-Glutamylcysteine synthetase (GCLC) and NAD (P) H: quinone oxidoreductase 1 (NQO1)) in heat-treated groups. CONCLUSIONS: These results suggest that Nrf2 displayed nuclear accumulation and protective activity in the process of heat treated-induced oxidative stress in mouse testes, indicating that Nrf2 might be a potential target for new drugs designed to protect germ cell and Leydig cell from oxidative stress. SN - 1477-7827 UR - https://www.unboundmedicine.com/medline/citation/23514035/Protective_effects_of_nuclear_factor_erythroid_2_related_factor_2_on_whole_body_heat_stress_induced_oxidative_damage_in_the_mouse_testis_ L2 - https://rbej.biomedcentral.com/articles/10.1186/1477-7827-11-23 DB - PRIME DP - Unbound Medicine ER -