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Contraindication of ART following a sperm FISH analysis, even though only 12% of the spermatozoa had enlarged heads.
Syst Biol Reprod Med 2013; 59(4):214-7SB

Abstract

We report on a couple with a five-year history of idiopathic primary infertility. Two early miscarriages had followed intrauterine insemination (IUI). The man's fertility was then re-evaluated, in order to establish whether or not IUI was the best treatment option. Although the semen parameters were normal (sperm concentration: 89 million/ml; progressive motility: 40%; percentage of typical forms: 20%), a computer-assisted sperm morphology analysis with strict criteria found that 12% of the spermatozoa had enlarged heads. All of the latter had a normal form and none had multiple flagella. Using fluorescence in situ hybridization (FISH) analysis, we found that the proportion of aneuploid and diploid spermatozoa was 78% for the sample as a whole and 68% for normally-shaped spermatozoa with a normal-sized head. Although treatment options are well documented for men with macrocephalic sperm head syndrom, there is no consensus on individuals with a low but non-negligible proportion of spermatozoa with enlarged heads. Here, our FISH results contraindicated the use of assisted reproductive technology with the man's sperm. The couple decided to resort to donor sperm.

Authors+Show Affiliations

Department of Reproductive Biology, Dreux Hospital, Dreux, France. brunoguthauser@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

23517468

Citation

Guthauser, Bruno, et al. "Contraindication of ART Following a Sperm FISH Analysis, Even Though Only 12% of the Spermatozoa Had Enlarged Heads." Systems Biology in Reproductive Medicine, vol. 59, no. 4, 2013, pp. 214-7.
Guthauser B, Boitrelle F, Albert M, et al. Contraindication of ART following a sperm FISH analysis, even though only 12% of the spermatozoa had enlarged heads. Syst Biol Reprod Med. 2013;59(4):214-7.
Guthauser, B., Boitrelle, F., Albert, M., Ketata, F., Meynant, C., Ferfouri, F., ... Vialard, F. (2013). Contraindication of ART following a sperm FISH analysis, even though only 12% of the spermatozoa had enlarged heads. Systems Biology in Reproductive Medicine, 59(4), pp. 214-7. doi:10.3109/19396368.2013.783646.
Guthauser B, et al. Contraindication of ART Following a Sperm FISH Analysis, Even Though Only 12% of the Spermatozoa Had Enlarged Heads. Syst Biol Reprod Med. 2013;59(4):214-7. PubMed PMID: 23517468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contraindication of ART following a sperm FISH analysis, even though only 12% of the spermatozoa had enlarged heads. AU - Guthauser,Bruno, AU - Boitrelle,Florence, AU - Albert,Martine, AU - Ketata,Fadoua, AU - Meynant,Celine, AU - Ferfouri,Fatma, AU - Selva,Jacqueline, AU - Vialard,Francois, Y1 - 2013/03/21/ PY - 2013/3/23/entrez PY - 2013/3/23/pubmed PY - 2014/2/19/medline SP - 214 EP - 7 JF - Systems biology in reproductive medicine JO - Syst Biol Reprod Med VL - 59 IS - 4 N2 - We report on a couple with a five-year history of idiopathic primary infertility. Two early miscarriages had followed intrauterine insemination (IUI). The man's fertility was then re-evaluated, in order to establish whether or not IUI was the best treatment option. Although the semen parameters were normal (sperm concentration: 89 million/ml; progressive motility: 40%; percentage of typical forms: 20%), a computer-assisted sperm morphology analysis with strict criteria found that 12% of the spermatozoa had enlarged heads. All of the latter had a normal form and none had multiple flagella. Using fluorescence in situ hybridization (FISH) analysis, we found that the proportion of aneuploid and diploid spermatozoa was 78% for the sample as a whole and 68% for normally-shaped spermatozoa with a normal-sized head. Although treatment options are well documented for men with macrocephalic sperm head syndrom, there is no consensus on individuals with a low but non-negligible proportion of spermatozoa with enlarged heads. Here, our FISH results contraindicated the use of assisted reproductive technology with the man's sperm. The couple decided to resort to donor sperm. SN - 1939-6376 UR - https://www.unboundmedicine.com/medline/citation/23517468/Contraindication_of_ART_following_a_sperm_FISH_analysis_even_though_only_12_of_the_spermatozoa_had_enlarged_heads_ L2 - http://www.tandfonline.com/doi/full/10.3109/19396368.2013.783646 DB - PRIME DP - Unbound Medicine ER -