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Mast cell expression of the serotonin1A receptor in guinea pig and human intestine.
Am J Physiol Gastrointest Liver Physiol. 2013 May 15; 304(10):G855-63.AJ

Abstract

Serotonin [5-hydroxytryptamine (5-HT)] is released from enterochromaffin cells in the mucosa of the small intestine. We tested a hypothesis that elevation of 5-HT in the environment of enteric mast cells might degranulate the mast cells and release mediators that become paracrine signals to the enteric nervous system, spinal afferents, and secretory glands. Western blotting, immunofluorescence, ELISA, and pharmacological analysis were used to study expression of 5-HT receptors by mast cells in the small intestine and action of 5-HT to degranulate the mast cells and release histamine in guinea pig small intestine and segments of human jejunum discarded during Roux-en-Y gastric bypass surgeries. Mast cells in human and guinea pig preparations expressed the 5-HT1A receptor. ELISA detected spontaneous release of histamine in guinea pig and human preparations. The selective 5-HT1A receptor agonist 8-hydroxy-PIPAT evoked release of histamine. A selective 5-HT1A receptor antagonist, WAY-100135, suppressed stimulation of histamine release by 5-HT or 8-hydroxy-PIPAT. Mast cell-stabilizing drugs, doxantrazole and cromolyn sodium, suppressed the release of histamine evoked by 5-HT or 8-hydroxy-PIPAT in guinea pig and human preparations. Our results support the hypothesis that serotonergic degranulation of enteric mast cells and release of preformed mediators, including histamine, are mediated by the 5-HT1A serotonergic receptor. Association of 5-HT with the pathophysiology of functional gastrointestinal disorders (e.g., irritable bowel syndrome) underlies a question of whether selective 5-HT1A receptor antagonists might have therapeutic application in disorders of this nature.

Authors+Show Affiliations

Dept. of Physiology and Cell Biology, College of Medicine and Public Health, The Ohio State Univ., 304 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23518679

Citation

Wang, Guo-Du, et al. "Mast Cell Expression of the serotonin1A Receptor in Guinea Pig and Human Intestine." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 304, no. 10, 2013, pp. G855-63.
Wang GD, Wang XY, Zou F, et al. Mast cell expression of the serotonin1A receptor in guinea pig and human intestine. Am J Physiol Gastrointest Liver Physiol. 2013;304(10):G855-63.
Wang, G. D., Wang, X. Y., Zou, F., Qu, M., Liu, S., Fei, G., Xia, Y., Needleman, B. J., Mikami, D. J., & Wood, J. D. (2013). Mast cell expression of the serotonin1A receptor in guinea pig and human intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology, 304(10), G855-63. https://doi.org/10.1152/ajpgi.00421.2012
Wang GD, et al. Mast Cell Expression of the serotonin1A Receptor in Guinea Pig and Human Intestine. Am J Physiol Gastrointest Liver Physiol. 2013 May 15;304(10):G855-63. PubMed PMID: 23518679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mast cell expression of the serotonin1A receptor in guinea pig and human intestine. AU - Wang,Guo-Du, AU - Wang,Xi-Yu, AU - Zou,Fei, AU - Qu,Meihua, AU - Liu,Sumei, AU - Fei,Guijun, AU - Xia,Yun, AU - Needleman,Bradley J, AU - Mikami,Dean J, AU - Wood,Jackie D, Y1 - 2013/03/21/ PY - 2013/3/23/entrez PY - 2013/3/23/pubmed PY - 2013/7/19/medline KW - enteric mast cells KW - enteric nervous system KW - functional gastrointestinal disorders KW - histamine KW - irritable bowel syndrome SP - G855 EP - 63 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 304 IS - 10 N2 - Serotonin [5-hydroxytryptamine (5-HT)] is released from enterochromaffin cells in the mucosa of the small intestine. We tested a hypothesis that elevation of 5-HT in the environment of enteric mast cells might degranulate the mast cells and release mediators that become paracrine signals to the enteric nervous system, spinal afferents, and secretory glands. Western blotting, immunofluorescence, ELISA, and pharmacological analysis were used to study expression of 5-HT receptors by mast cells in the small intestine and action of 5-HT to degranulate the mast cells and release histamine in guinea pig small intestine and segments of human jejunum discarded during Roux-en-Y gastric bypass surgeries. Mast cells in human and guinea pig preparations expressed the 5-HT1A receptor. ELISA detected spontaneous release of histamine in guinea pig and human preparations. The selective 5-HT1A receptor agonist 8-hydroxy-PIPAT evoked release of histamine. A selective 5-HT1A receptor antagonist, WAY-100135, suppressed stimulation of histamine release by 5-HT or 8-hydroxy-PIPAT. Mast cell-stabilizing drugs, doxantrazole and cromolyn sodium, suppressed the release of histamine evoked by 5-HT or 8-hydroxy-PIPAT in guinea pig and human preparations. Our results support the hypothesis that serotonergic degranulation of enteric mast cells and release of preformed mediators, including histamine, are mediated by the 5-HT1A serotonergic receptor. Association of 5-HT with the pathophysiology of functional gastrointestinal disorders (e.g., irritable bowel syndrome) underlies a question of whether selective 5-HT1A receptor antagonists might have therapeutic application in disorders of this nature. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/23518679/Mast_cell_expression_of_the_serotonin1A_receptor_in_guinea_pig_and_human_intestine_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00421.2012?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -