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In vitro stability of free and glucuronidated cannabinoids in blood and plasma following controlled smoked cannabis.
Clin Chem. 2013 Jul; 59(7):1108-17.CC

Abstract

BACKGROUND

Blood and plasma cannabinoid stability is important for test interpretation and is best studied in authentic rather than fortified samples.

METHODS

Low and high blood and plasma pools were created for each of 10 participants after they smoked a cannabis cigarette. The stabilities of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), cannabinol (CBN), THC-glucuronide, and THCCOOH-glucuronide were determined after 1 week at room temperature; 1, 2, 4, 12, and 26 (±2) weeks at 4 °C; and 1, 2, 4, 12, 26 (±2), and 52 (±4) weeks at -20 °C. Stability was assessed by Friedman test.

RESULTS

Numbers of THC-glucuronide and CBD-positive blood samples were insufficient to assess stability. In blood, 11-OH-THC and CBN were stable for 1 week at room temperature, whereas THC and THCCOOH-glucuronide decreased and THCCOOH increased. In blood, THC, THCCOOH-glucuronide, THCCOOH, 11-OH-THC, and CBN were stable for 12, 4, 4, 12, and 26 weeks, respectively, at 4 °C and 12, 12, 26, 26, and 52 weeks at -20 °C. In plasma, THC-glucuronide, THC, CBN, and CBD were stable for 1 week at room temperature, whereas THCCOOH-glucuronide and 11-OH-THC decreased and THCCOOH increased. In plasma, THC-glucuronide, THC, THCCOOH-glucuronide, THCCOOH, 11-OH-THC, CBN, and CBD were stable for 26, 26, 2, 2, 26, 12, and 26 weeks, respectively, at 4 °C and 52, 52, 26, 26, 52, 52, and 52 weeks, respectively, at -20 °C.

CONCLUSIONS

Blood and plasma samples should be stored at -20 °C for no more than 3 and 6 months, respectively, to assure accurate cannabinoid quantitative results.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD. Aegis Sciences Corp., Nashville, TN.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD. Aegis Sciences Corp., Nashville, TN.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD.

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

23519966

Citation

Scheidweiler, Karl B., et al. "In Vitro Stability of Free and Glucuronidated Cannabinoids in Blood and Plasma Following Controlled Smoked Cannabis." Clinical Chemistry, vol. 59, no. 7, 2013, pp. 1108-17.
Scheidweiler KB, Schwope DM, Karschner EL, et al. In vitro stability of free and glucuronidated cannabinoids in blood and plasma following controlled smoked cannabis. Clin Chem. 2013;59(7):1108-17.
Scheidweiler, K. B., Schwope, D. M., Karschner, E. L., Desrosiers, N. A., Gorelick, D. A., & Huestis, M. A. (2013). In vitro stability of free and glucuronidated cannabinoids in blood and plasma following controlled smoked cannabis. Clinical Chemistry, 59(7), 1108-17. https://doi.org/10.1373/clinchem.2012.201467
Scheidweiler KB, et al. In Vitro Stability of Free and Glucuronidated Cannabinoids in Blood and Plasma Following Controlled Smoked Cannabis. Clin Chem. 2013;59(7):1108-17. PubMed PMID: 23519966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro stability of free and glucuronidated cannabinoids in blood and plasma following controlled smoked cannabis. AU - Scheidweiler,Karl B, AU - Schwope,David M, AU - Karschner,Erin L, AU - Desrosiers,Nathalie A, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2013/03/21/ PY - 2013/3/23/entrez PY - 2013/3/23/pubmed PY - 2013/8/28/medline SP - 1108 EP - 17 JF - Clinical chemistry JO - Clin Chem VL - 59 IS - 7 N2 - BACKGROUND: Blood and plasma cannabinoid stability is important for test interpretation and is best studied in authentic rather than fortified samples. METHODS: Low and high blood and plasma pools were created for each of 10 participants after they smoked a cannabis cigarette. The stabilities of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), cannabinol (CBN), THC-glucuronide, and THCCOOH-glucuronide were determined after 1 week at room temperature; 1, 2, 4, 12, and 26 (±2) weeks at 4 °C; and 1, 2, 4, 12, 26 (±2), and 52 (±4) weeks at -20 °C. Stability was assessed by Friedman test. RESULTS: Numbers of THC-glucuronide and CBD-positive blood samples were insufficient to assess stability. In blood, 11-OH-THC and CBN were stable for 1 week at room temperature, whereas THC and THCCOOH-glucuronide decreased and THCCOOH increased. In blood, THC, THCCOOH-glucuronide, THCCOOH, 11-OH-THC, and CBN were stable for 12, 4, 4, 12, and 26 weeks, respectively, at 4 °C and 12, 12, 26, 26, and 52 weeks at -20 °C. In plasma, THC-glucuronide, THC, CBN, and CBD were stable for 1 week at room temperature, whereas THCCOOH-glucuronide and 11-OH-THC decreased and THCCOOH increased. In plasma, THC-glucuronide, THC, THCCOOH-glucuronide, THCCOOH, 11-OH-THC, CBN, and CBD were stable for 26, 26, 2, 2, 26, 12, and 26 weeks, respectively, at 4 °C and 52, 52, 26, 26, 52, 52, and 52 weeks, respectively, at -20 °C. CONCLUSIONS: Blood and plasma samples should be stored at -20 °C for no more than 3 and 6 months, respectively, to assure accurate cannabinoid quantitative results. SN - 1530-8561 UR - https://www.unboundmedicine.com/medline/citation/23519966/In_vitro_stability_of_free_and_glucuronidated_cannabinoids_in_blood_and_plasma_following_controlled_smoked_cannabis_ L2 - https://academic.oup.com/clinchem/article-lookup/doi/10.1373/clinchem.2012.201467 DB - PRIME DP - Unbound Medicine ER -