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Spatial heterogeneity of cyanobacterial communities and genetic variation of microcystis populations within large, shallow eutrophic lakes (Lake Taihu and Lake Chaohu, China).
J Environ Sci (China). 2012; 24(10):1832-42.JE

Abstract

Cyanobacteria, specifically Microcystis, usually form massive blooms in eutrophic freshwater lakes. Cyanobacterial samples were collected from eight sites of both Lake Taihu and Lake Chaohu in late summer to determine the diversity and distribution pattern of cyanobacteria and Microcystis in large, shallow, entropic lakes with significant spatial heterogeneity and long-term Microcystis bloom. Molecular methods based on denaturing gradient gel electrophoresis and clone library analysis were used. A similar heterogeneous distribution pattern of cyanobacteria in both lakes was observed. Most parts of these two lakes with high trophic level were dominated by Microcystis. However, in the regions with low trophic levels as well as low concentrations of chlorophyll a, Synechococcus occupied a considerable percentage. Different morphospecies and genotypes dominated the bloom-forming Microcystis populations in these two lakes. Microcystis viridis and Microcystis novacekii were dominant in Lake Chaohu, whereas Microcystis flos-aquae was dominant in Lake Taihu. Only 2 of thel3 Microcystis operational taxonomic units were shared between these two lakes. Analysis of molecular variance based on 16S to 23S internal transcribed spacer sequences indicated the significAnt genetic differentiation of Microcystis between these two lakes (F(ST) = 0.19, p < 0.001). However, only 19.46% of the genetic variability was explained by the population variation between lakes, whereas most (80.54%) of the genetic variability occurred within the lakes. Phylogenetic analysis revealed no phylogeographic structure of Microcystis population in these two lakes, as illustrated by their cosmopolitan nature. Our results revealed that spatial heterogeneity within lakes has more impact on the cyanobacterial diversity than geographical isolation in a local scale.

Authors+Show Affiliations

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China. wcyfhgl@126.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23520854

Citation

Cai, Yuanfeng, et al. "Spatial Heterogeneity of Cyanobacterial Communities and Genetic Variation of Microcystis Populations Within Large, Shallow Eutrophic Lakes (Lake Taihu and Lake Chaohu, China)." Journal of Environmental Sciences (China), vol. 24, no. 10, 2012, pp. 1832-42.
Cai Y, Kong F, Shi L, et al. Spatial heterogeneity of cyanobacterial communities and genetic variation of microcystis populations within large, shallow eutrophic lakes (Lake Taihu and Lake Chaohu, China). J Environ Sci (China). 2012;24(10):1832-42.
Cai, Y., Kong, F., Shi, L., & Yu, Y. (2012). Spatial heterogeneity of cyanobacterial communities and genetic variation of microcystis populations within large, shallow eutrophic lakes (Lake Taihu and Lake Chaohu, China). Journal of Environmental Sciences (China), 24(10), 1832-42.
Cai Y, et al. Spatial Heterogeneity of Cyanobacterial Communities and Genetic Variation of Microcystis Populations Within Large, Shallow Eutrophic Lakes (Lake Taihu and Lake Chaohu, China). J Environ Sci (China). 2012;24(10):1832-42. PubMed PMID: 23520854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatial heterogeneity of cyanobacterial communities and genetic variation of microcystis populations within large, shallow eutrophic lakes (Lake Taihu and Lake Chaohu, China). AU - Cai,Yuanfeng, AU - Kong,Fanxiang, AU - Shi,Limei, AU - Yu,Yang, PY - 2013/3/26/entrez PY - 2012/1/1/pubmed PY - 2013/5/3/medline SP - 1832 EP - 42 JF - Journal of environmental sciences (China) JO - J Environ Sci (China) VL - 24 IS - 10 N2 - Cyanobacteria, specifically Microcystis, usually form massive blooms in eutrophic freshwater lakes. Cyanobacterial samples were collected from eight sites of both Lake Taihu and Lake Chaohu in late summer to determine the diversity and distribution pattern of cyanobacteria and Microcystis in large, shallow, entropic lakes with significant spatial heterogeneity and long-term Microcystis bloom. Molecular methods based on denaturing gradient gel electrophoresis and clone library analysis were used. A similar heterogeneous distribution pattern of cyanobacteria in both lakes was observed. Most parts of these two lakes with high trophic level were dominated by Microcystis. However, in the regions with low trophic levels as well as low concentrations of chlorophyll a, Synechococcus occupied a considerable percentage. Different morphospecies and genotypes dominated the bloom-forming Microcystis populations in these two lakes. Microcystis viridis and Microcystis novacekii were dominant in Lake Chaohu, whereas Microcystis flos-aquae was dominant in Lake Taihu. Only 2 of thel3 Microcystis operational taxonomic units were shared between these two lakes. Analysis of molecular variance based on 16S to 23S internal transcribed spacer sequences indicated the significAnt genetic differentiation of Microcystis between these two lakes (F(ST) = 0.19, p < 0.001). However, only 19.46% of the genetic variability was explained by the population variation between lakes, whereas most (80.54%) of the genetic variability occurred within the lakes. Phylogenetic analysis revealed no phylogeographic structure of Microcystis population in these two lakes, as illustrated by their cosmopolitan nature. Our results revealed that spatial heterogeneity within lakes has more impact on the cyanobacterial diversity than geographical isolation in a local scale. SN - 1001-0742 UR - https://www.unboundmedicine.com/medline/citation/23520854/Spatial_heterogeneity_of_cyanobacterial_communities_and_genetic_variation_of_microcystis_populations_within_large_shallow_eutrophic_lakes__Lake_Taihu_and_Lake_Chaohu_China__ DB - PRIME DP - Unbound Medicine ER -