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Distribution of several activating and inhibitory receptors on CD3(-)CD56(+) NK cells in regional lymph nodes of melanoma patients.

Abstract

BACKGROUND

Natural killer (NK) cells, as the main effector subpopulation of the innate immune system, play an important role in the control of the rise and spread of malignant tumors. Regional lymph nodes (LN) represent the first immunologic barrier to tumor metastasis. Since there are scarce data on NK cells from regional LN of cancer patients, the aim of this study was to investigate the expression of several activating and inhibitory receptors on the entire NK cell population as well as their CD3(-)CD56(dim) and CD3(-)CD56(bright) functional NK subsets from regional LN of melanoma patients.

MATERIALS AND METHODS

Mononuclear cells were isolated from 50 regional LN of melanoma patients. The expression of several receptors on NK cells and their functional subsets was analyzed by flow cytometry.

RESULTS

We show increased percentages of CD3(-)CD56(+) NK cells in involved LN compared with uninvolved LN, mostly in favor of the CD56(dim) NK cell subset. NK cells in involved LN express similar levels of activating receptor NKG2D, while the level of another activating receptor, CD16, is increased compared with uninvolved LN. Regarding the expression of inhibitory NK cell receptors, we show increased CD158b, but similar low CD158a, inhibitory killer Ig-like cell receptor expression in involved LN compared with uninvolved LN. Furthermore, NK cells in involved compared with uninvolved LN displayed increased CD69 early activation antigen expression.

CONCLUSIONS

Our results indicate that with tumor infiltration into regional LN of melanoma patients, NK cells, mostly of the CD56(dim) subset, are recruited into draining LN. The invading NK cells show counterbalance of the increased expression of CD16 activating receptor and increased CD158b inhibitory killer Ig-like cell receptor.

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  • Authors+Show Affiliations

    ,

    Institute of Oncology and Radiology of Serbia, Belgrade, Serbia.

    , , , ,

    Source

    The Journal of surgical research 183:2 2013 Aug pg 860-8

    MeSH

    Adult
    Aged
    Aged, 80 and over
    CD3 Complex
    CD56 Antigen
    Female
    Humans
    Killer Cells, Natural
    Lymph Nodes
    Lymphatic Metastasis
    Male
    Melanoma
    Middle Aged
    NK Cell Lectin-Like Receptor Subfamily K
    Receptors, IgG
    Receptors, KIR2DL1
    Receptors, KIR2DL3
    Receptors, Natural Killer Cell
    Skin Neoplasms

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23522458

    Citation

    Vuletić, Ana, et al. "Distribution of Several Activating and Inhibitory Receptors on CD3(-)CD56(+) NK Cells in Regional Lymph Nodes of Melanoma Patients." The Journal of Surgical Research, vol. 183, no. 2, 2013, pp. 860-8.
    Vuletić A, Jurišić V, Jovanić I, et al. Distribution of several activating and inhibitory receptors on CD3(-)CD56(+) NK cells in regional lymph nodes of melanoma patients. J Surg Res. 2013;183(2):860-8.
    Vuletić, A., Jurišić, V., Jovanić, I., Milovanović, Z., Nikolić, S., & Konjević, G. (2013). Distribution of several activating and inhibitory receptors on CD3(-)CD56(+) NK cells in regional lymph nodes of melanoma patients. The Journal of Surgical Research, 183(2), pp. 860-8. doi:10.1016/j.jss.2013.02.025.
    Vuletić A, et al. Distribution of Several Activating and Inhibitory Receptors on CD3(-)CD56(+) NK Cells in Regional Lymph Nodes of Melanoma Patients. J Surg Res. 2013;183(2):860-8. PubMed PMID: 23522458.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Distribution of several activating and inhibitory receptors on CD3(-)CD56(+) NK cells in regional lymph nodes of melanoma patients. AU - Vuletić,Ana, AU - Jurišić,Vladimir, AU - Jovanić,Irena, AU - Milovanović,Zorka, AU - Nikolić,Srđan, AU - Konjević,Gordana, Y1 - 2013/03/13/ PY - 2012/09/30/received PY - 2013/01/19/revised PY - 2013/02/15/accepted PY - 2013/3/26/entrez PY - 2013/3/26/pubmed PY - 2013/10/18/medline KW - CD16 KW - KIR KW - Lymph nodes KW - Melanoma KW - NK cell receptors KW - NK cells KW - NKG2D SP - 860 EP - 8 JF - The Journal of surgical research JO - J. Surg. Res. VL - 183 IS - 2 N2 - BACKGROUND: Natural killer (NK) cells, as the main effector subpopulation of the innate immune system, play an important role in the control of the rise and spread of malignant tumors. Regional lymph nodes (LN) represent the first immunologic barrier to tumor metastasis. Since there are scarce data on NK cells from regional LN of cancer patients, the aim of this study was to investigate the expression of several activating and inhibitory receptors on the entire NK cell population as well as their CD3(-)CD56(dim) and CD3(-)CD56(bright) functional NK subsets from regional LN of melanoma patients. MATERIALS AND METHODS: Mononuclear cells were isolated from 50 regional LN of melanoma patients. The expression of several receptors on NK cells and their functional subsets was analyzed by flow cytometry. RESULTS: We show increased percentages of CD3(-)CD56(+) NK cells in involved LN compared with uninvolved LN, mostly in favor of the CD56(dim) NK cell subset. NK cells in involved LN express similar levels of activating receptor NKG2D, while the level of another activating receptor, CD16, is increased compared with uninvolved LN. Regarding the expression of inhibitory NK cell receptors, we show increased CD158b, but similar low CD158a, inhibitory killer Ig-like cell receptor expression in involved LN compared with uninvolved LN. Furthermore, NK cells in involved compared with uninvolved LN displayed increased CD69 early activation antigen expression. CONCLUSIONS: Our results indicate that with tumor infiltration into regional LN of melanoma patients, NK cells, mostly of the CD56(dim) subset, are recruited into draining LN. The invading NK cells show counterbalance of the increased expression of CD16 activating receptor and increased CD158b inhibitory killer Ig-like cell receptor. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/23522458/Distribution_of_several_activating_and_inhibitory_receptors_on CD3___CD56_+__NK_cells_in_regional_lymph_nodes_of_melanoma_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(13)00105-4 DB - PRIME DP - Unbound Medicine ER -