Tags

Type your tag names separated by a space and hit enter

Analysis of ciliary assembly and function in planaria.
Methods Enzymol. 2013; 525:245-64.ME

Abstract

Planarians are free-living invertebrates that employ motile cilia for locomotion. Specifically, cilia that populate the ventral epithelium of the planarian body are highly conserved, with a 9+2 axoneme and a full complement of inner and outer arm dynein motors. The abundance of cilia on the planarian body, their unique accessibility, and high degree of conservation make this organism an attractive experimental model system for cilia biology. Moreover, planarians are genetically amenable and defects that compromise the function and structure of the cilia are not detrimental for their overall health, making them an ideal system for cilia gene loss-of-function studies. In this chapter, we provide information for introducing and maintaining planarians for experimental purposes in the laboratory and describe protocols for RNAi-induced gene knockdown studies. Furthermore, we elaborate on different imaging techniques used to analyze cilia physiology and structure, including live video microscopy, immunofluorescence analysis, and electron microscopy. Last, we provide assays for evaluating physical parameters of ciliary motility, including quantification of planarian gliding locomotion and measurement of ciliary beat frequency.

Authors+Show Affiliations

Department of Genetics, Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, USA. panteleimon.rompolas@yale.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23522473

Citation

Rompolas, Panteleimon, et al. "Analysis of Ciliary Assembly and Function in Planaria." Methods in Enzymology, vol. 525, 2013, pp. 245-64.
Rompolas P, Azimzadeh J, Marshall WF, et al. Analysis of ciliary assembly and function in planaria. Meth Enzymol. 2013;525:245-64.
Rompolas, P., Azimzadeh, J., Marshall, W. F., & King, S. M. (2013). Analysis of ciliary assembly and function in planaria. Methods in Enzymology, 525, 245-64. https://doi.org/10.1016/B978-0-12-397944-5.00012-2
Rompolas P, et al. Analysis of Ciliary Assembly and Function in Planaria. Meth Enzymol. 2013;525:245-64. PubMed PMID: 23522473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of ciliary assembly and function in planaria. AU - Rompolas,Panteleimon, AU - Azimzadeh,Juliette, AU - Marshall,Wallace F, AU - King,Stephen M, PY - 2013/3/26/entrez PY - 2013/3/26/pubmed PY - 2013/9/12/medline SP - 245 EP - 64 JF - Methods in enzymology JO - Meth. Enzymol. VL - 525 N2 - Planarians are free-living invertebrates that employ motile cilia for locomotion. Specifically, cilia that populate the ventral epithelium of the planarian body are highly conserved, with a 9+2 axoneme and a full complement of inner and outer arm dynein motors. The abundance of cilia on the planarian body, their unique accessibility, and high degree of conservation make this organism an attractive experimental model system for cilia biology. Moreover, planarians are genetically amenable and defects that compromise the function and structure of the cilia are not detrimental for their overall health, making them an ideal system for cilia gene loss-of-function studies. In this chapter, we provide information for introducing and maintaining planarians for experimental purposes in the laboratory and describe protocols for RNAi-induced gene knockdown studies. Furthermore, we elaborate on different imaging techniques used to analyze cilia physiology and structure, including live video microscopy, immunofluorescence analysis, and electron microscopy. Last, we provide assays for evaluating physical parameters of ciliary motility, including quantification of planarian gliding locomotion and measurement of ciliary beat frequency. SN - 1557-7988 UR - https://www.unboundmedicine.com/medline/citation/23522473/Analysis_of_ciliary_assembly_and_function_in_planaria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/B978-0-12-397944-5.00012-2 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.