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Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans.
J Ethnopharmacol 2013; 147(2):366-72JE

Abstract

AIM OF THE STUDY

As a traditional Chinese medicine, seed of Platycladus orientalis(Linnaeus) Franco has been extensively used as a tonic and sedative remedy. The present study was conducted to investigate whether lifespan was extended and the mechanisms of n-butanol extract from seed of Platycladus orientalis (BSPO) in Caenorhabditis elegans. The findings could provide the pharmacological basis for a treatment in traditional medicine.

MATERIALS AND METHODS

Lifespan extension by BSPO was evaluated under normal culture conditions and in a stress test. A possible mechanism of the anti-aging effect of BSPO, a change in the stress-resistance of related proteins, was also investigated in C. elegans.

RESULTS

It has been shown that BSPO could significantly extend lifespan of C. elegans in a concentration dependent manner under normal culture conditions and stress. Further studies demonstrated that BSPO treatment significantly decreased reactive oxygen species (ROS) accumulation, up-regulated resistance to stress of related proteins, including glutathione S-transferase-4 (GST-4) and heat shock protein-16.2 (HSP-16.2), and reduced the amount of lipofuscin in transgenic C. elegans.

CONCLUSION

These results indicated that BSPO extended the lifespan, which could be attributed to its direct ROS scavenging activity, reducing the amount of lipofuscin and increasing the expression of gens associated with resistance to stress. These obtained data provided valuable support for traditional clinical practice to extend lifespan and to provide tonic remedy.

Authors+Show Affiliations

Guangdong Key Laboratory of Plant Resources, Guangzhou Quality R&D Center of Traditional Chinese Medicine, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23523941

Citation

Liu, Haibin, et al. "Lifespan Extension By N-butanol Extract From Seed of Platycladus Orientalis in Caenorhabditis Elegans." Journal of Ethnopharmacology, vol. 147, no. 2, 2013, pp. 366-72.
Liu H, Liang F, Su W, et al. Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. J Ethnopharmacol. 2013;147(2):366-72.
Liu, H., Liang, F., Su, W., Wang, N., Lv, M., Li, P., ... Wang, Y. (2013). Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. Journal of Ethnopharmacology, 147(2), pp. 366-72. doi:10.1016/j.jep.2013.03.019.
Liu H, et al. Lifespan Extension By N-butanol Extract From Seed of Platycladus Orientalis in Caenorhabditis Elegans. J Ethnopharmacol. 2013 May 20;147(2):366-72. PubMed PMID: 23523941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans. AU - Liu,Haibin, AU - Liang,Fengyin, AU - Su,Weiwei, AU - Wang,Ning, AU - Lv,Mingliang, AU - Li,Peibo, AU - Pei,Zhong, AU - Zhang,Yan, AU - Xie,Xiang-Qun, AU - Wang,Lirong, AU - Wang,Yonggang, Y1 - 2013/03/21/ PY - 2012/11/20/received PY - 2013/01/29/revised PY - 2013/03/07/accepted PY - 2013/3/26/entrez PY - 2013/3/26/pubmed PY - 2013/10/24/medline SP - 366 EP - 72 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 147 IS - 2 N2 - AIM OF THE STUDY: As a traditional Chinese medicine, seed of Platycladus orientalis(Linnaeus) Franco has been extensively used as a tonic and sedative remedy. The present study was conducted to investigate whether lifespan was extended and the mechanisms of n-butanol extract from seed of Platycladus orientalis (BSPO) in Caenorhabditis elegans. The findings could provide the pharmacological basis for a treatment in traditional medicine. MATERIALS AND METHODS: Lifespan extension by BSPO was evaluated under normal culture conditions and in a stress test. A possible mechanism of the anti-aging effect of BSPO, a change in the stress-resistance of related proteins, was also investigated in C. elegans. RESULTS: It has been shown that BSPO could significantly extend lifespan of C. elegans in a concentration dependent manner under normal culture conditions and stress. Further studies demonstrated that BSPO treatment significantly decreased reactive oxygen species (ROS) accumulation, up-regulated resistance to stress of related proteins, including glutathione S-transferase-4 (GST-4) and heat shock protein-16.2 (HSP-16.2), and reduced the amount of lipofuscin in transgenic C. elegans. CONCLUSION: These results indicated that BSPO extended the lifespan, which could be attributed to its direct ROS scavenging activity, reducing the amount of lipofuscin and increasing the expression of gens associated with resistance to stress. These obtained data provided valuable support for traditional clinical practice to extend lifespan and to provide tonic remedy. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23523941/Lifespan_extension_by_n_butanol_extract_from_seed_of_Platycladus_orientalis_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00162-1 DB - PRIME DP - Unbound Medicine ER -