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Effects of neuregulin-1β on growth-associated protein 43 expression in dorsal root ganglion neurons with excitotoxicity induced by glutamate in vitro.
Neurosci Res. 2013 May-Jun; 76(1-2):22-30.NR

Abstract

Neuregulin-1β (NRG-1β) is a growth factor with potent neuroprotective capacity. Growth-associated protein 43 (GAP-43) is expressed in dorsal root ganglion (DRG) neurons and an indicator of neuronal survival in vitro. The purpose of present study is to evaluate the effects of NRG-1β on GAP-43 expression in DRG neurons with excitotoxicity induced by glutamate (Glu) in vitro. The phosphatidylinositol 3-kinase (PI3K)/Akt and extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathways involved in these effects were also determined. Embryonic rat DRG neurons were treated with Glu in the absence or presence of NRG-1β and PI3K inhibitor LY294002 and/or ERK1/2 inhibitor PD98059. After that, GAP-43 mRNA and GAP-43 protein levels were analyzed by real time-PCR and western blot assay, respectively. GAP-43 expression in situ was determined by immunofluorescent labeling. The results showed that the decreased GAP-43 levels induced by Glu could be partially reversed by the presence of NRG-1β. Inhibitors (LY294002, PD98059) either alone or in combination blocked the effects of NRG-1β. These data provide new insights of the actions of NRG-1β in sensory neurons.

Authors+Show Affiliations

Faculty of Clinical Medicine, Shandong University School of Medicine, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23524246

Citation

Li, Yunfeng, et al. "Effects of Neuregulin-1β On Growth-associated Protein 43 Expression in Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate in Vitro." Neuroscience Research, vol. 76, no. 1-2, 2013, pp. 22-30.
Li Y, Li H, Liu G, et al. Effects of neuregulin-1β on growth-associated protein 43 expression in dorsal root ganglion neurons with excitotoxicity induced by glutamate in vitro. Neurosci Res. 2013;76(1-2):22-30.
Li, Y., Li, H., Liu, G., & Liu, Z. (2013). Effects of neuregulin-1β on growth-associated protein 43 expression in dorsal root ganglion neurons with excitotoxicity induced by glutamate in vitro. Neuroscience Research, 76(1-2), 22-30. https://doi.org/10.1016/j.neures.2013.02.012
Li Y, et al. Effects of Neuregulin-1β On Growth-associated Protein 43 Expression in Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate in Vitro. Neurosci Res. 2013 May-Jun;76(1-2):22-30. PubMed PMID: 23524246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of neuregulin-1β on growth-associated protein 43 expression in dorsal root ganglion neurons with excitotoxicity induced by glutamate in vitro. AU - Li,Yunfeng, AU - Li,Hao, AU - Liu,Guixiang, AU - Liu,Zhen, Y1 - 2013/03/22/ PY - 2012/10/07/received PY - 2013/02/18/revised PY - 2013/02/19/accepted PY - 2013/3/26/entrez PY - 2013/3/26/pubmed PY - 2014/1/18/medline SP - 22 EP - 30 JF - Neuroscience research JO - Neurosci Res VL - 76 IS - 1-2 N2 - Neuregulin-1β (NRG-1β) is a growth factor with potent neuroprotective capacity. Growth-associated protein 43 (GAP-43) is expressed in dorsal root ganglion (DRG) neurons and an indicator of neuronal survival in vitro. The purpose of present study is to evaluate the effects of NRG-1β on GAP-43 expression in DRG neurons with excitotoxicity induced by glutamate (Glu) in vitro. The phosphatidylinositol 3-kinase (PI3K)/Akt and extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathways involved in these effects were also determined. Embryonic rat DRG neurons were treated with Glu in the absence or presence of NRG-1β and PI3K inhibitor LY294002 and/or ERK1/2 inhibitor PD98059. After that, GAP-43 mRNA and GAP-43 protein levels were analyzed by real time-PCR and western blot assay, respectively. GAP-43 expression in situ was determined by immunofluorescent labeling. The results showed that the decreased GAP-43 levels induced by Glu could be partially reversed by the presence of NRG-1β. Inhibitors (LY294002, PD98059) either alone or in combination blocked the effects of NRG-1β. These data provide new insights of the actions of NRG-1β in sensory neurons. SN - 1872-8111 UR - https://www.unboundmedicine.com/medline/citation/23524246/Effects_of_neuregulin_1β_on_growth_associated_protein_43_expression_in_dorsal_root_ganglion_neurons_with_excitotoxicity_induced_by_glutamate_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(13)00081-3 DB - PRIME DP - Unbound Medicine ER -