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Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments.
PLoS One. 2013; 8(3):e59889.Plos

Abstract

Highly pathogenic avian influenza H5N1 viruses have devastated the poultry industry in many countries of the eastern hemisphere. Occasionally H5N1 viruses cross the species barrier and infect humans, sometimes with a severe clinical outcome. When this happens, there is a chance of reassortment between H5N1 and human influenza viruses. To assess the potential of H5N1 viruses to reassort with contemporary human influenza viruses (H1N1, H3N2 and pandemic H1N1), we used an in vitro selection method to generate reassortant viruses, that contained the H5 hemagglutinin gene, and that have a replication advantage in vitro. We found that the neuraminidase and matrix gene segments of human influenza viruses were preferentially selected by H5 viruses. However, these H5 reassortant viruses did not show a marked increase in replication in MDCK cells and human bronchial epithelial cells. In ferrets, inoculation with a mixture of H5N1-pandemic H1N1 reassortant viruses resulted in outgrowth of reassortant H5 viruses that had incorporated the neuraminidase and matrix gene segment of pandemic 2009 H1N1. This virus was not transmitted via aerosols or respiratory droplets to naïve recipient ferrets. Altogether, these data emphasize the potential of avian H5N1 viruses to reassort with contemporary human influenza viruses. The neuraminidase and matrix gene segments of human influenza viruses showed the highest genetic compatibility with HPAI H5N1 virus.

Authors+Show Affiliations

Viroscience Lab, Postgraduate School Molecular Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23527283

Citation

Schrauwen, Eefje J A., et al. "Reassortment Between Avian H5N1 and Human Influenza Viruses Is Mainly Restricted to the Matrix and Neuraminidase Gene Segments." PloS One, vol. 8, no. 3, 2013, pp. e59889.
Schrauwen EJ, Bestebroer TM, Rimmelzwaan GF, et al. Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments. PLoS ONE. 2013;8(3):e59889.
Schrauwen, E. J., Bestebroer, T. M., Rimmelzwaan, G. F., Osterhaus, A. D., Fouchier, R. A., & Herfst, S. (2013). Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments. PloS One, 8(3), e59889. https://doi.org/10.1371/journal.pone.0059889
Schrauwen EJ, et al. Reassortment Between Avian H5N1 and Human Influenza Viruses Is Mainly Restricted to the Matrix and Neuraminidase Gene Segments. PLoS ONE. 2013;8(3):e59889. PubMed PMID: 23527283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments. AU - Schrauwen,Eefje J A, AU - Bestebroer,Theo M, AU - Rimmelzwaan,Guus F, AU - Osterhaus,Albert D M E, AU - Fouchier,Ron A M, AU - Herfst,Sander, Y1 - 2013/03/20/ PY - 2012/11/09/received PY - 2013/02/19/accepted PY - 2013/3/26/entrez PY - 2013/3/26/pubmed PY - 2013/9/18/medline SP - e59889 EP - e59889 JF - PloS one JO - PLoS ONE VL - 8 IS - 3 N2 - Highly pathogenic avian influenza H5N1 viruses have devastated the poultry industry in many countries of the eastern hemisphere. Occasionally H5N1 viruses cross the species barrier and infect humans, sometimes with a severe clinical outcome. When this happens, there is a chance of reassortment between H5N1 and human influenza viruses. To assess the potential of H5N1 viruses to reassort with contemporary human influenza viruses (H1N1, H3N2 and pandemic H1N1), we used an in vitro selection method to generate reassortant viruses, that contained the H5 hemagglutinin gene, and that have a replication advantage in vitro. We found that the neuraminidase and matrix gene segments of human influenza viruses were preferentially selected by H5 viruses. However, these H5 reassortant viruses did not show a marked increase in replication in MDCK cells and human bronchial epithelial cells. In ferrets, inoculation with a mixture of H5N1-pandemic H1N1 reassortant viruses resulted in outgrowth of reassortant H5 viruses that had incorporated the neuraminidase and matrix gene segment of pandemic 2009 H1N1. This virus was not transmitted via aerosols or respiratory droplets to naïve recipient ferrets. Altogether, these data emphasize the potential of avian H5N1 viruses to reassort with contemporary human influenza viruses. The neuraminidase and matrix gene segments of human influenza viruses showed the highest genetic compatibility with HPAI H5N1 virus. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23527283/Reassortment_between_Avian_H5N1_and_human_influenza_viruses_is_mainly_restricted_to_the_matrix_and_neuraminidase_gene_segments_ L2 - http://dx.plos.org/10.1371/journal.pone.0059889 DB - PRIME DP - Unbound Medicine ER -